Micro-screening apparatus, process, and products

Patent metadata

Field	Value
Publication number	US-10526600-B2
Application number	US-201615050130-A
Country	US
Kind code	B2
Filing date	Feb 22, 2016
Priority date	Feb 22, 2015
Publication date	Jan 7, 2020
Grant date	Jan 7, 2020

Abstract

Microcavity arrays and methods for quantitative biochemical and biophysical analysis of populations of biological variants. Examples include high-throughput analysis of cells and protein products use a range of fluorescent assays, including binding-affinity measurement and time-resolved enzyme assays. Laser-based extraction of microcavity contents.

First claim

What is claimed is:

1. A method for extracting the contents of a cavity of a microcavity array, the method comprising:
focusing electromagnetic radiation from a pulsed diode laser at an electromagnetic radiation absorbing material associated with the cavity, wherein the pulsed diode laser delivers electromagnetic radiation to the material in 2-20 pulses having pulse length of 1-10 milliseconds with a pulse separation of 10 to 100 millisecond;
extracting the contents of a cavity comprising cells from at least one cavity of the array onto a capture surface; and
contacting the capture surface with a culture matrix comprising media that sustains the viability of the cells.

2. The method of claim 1 , where the culture matrix comprises agarose.

3. The method of claim 1 , wherein the electromagnetic radiation absorbing material comprises microparticles in the cavity.

4. The method of claim 1 , wherein the array is constructed of an opaque material comprising a lead glass that has been reduced in a hydrogen atmosphere.

5. The method of claim 1 , wherein the laser is focused at a surface of the material in contact with a liquid in the cavity.

6. The method of claim 1 , wherein the laser directs the focus of the electromagnetic radiation at the material located at a meniscus of the liquid.

7. The method of claim 1 , wherein the focusing of the electromagnetic radiation at the material comprises applying the electromagnetic radiation to the radiation absorbing material to avoid heating a liquid that is not in contact with the material.

Assignees

• Univ Leland Stanford Junior

Inventors

• Baer Thomas M
• Cochran Jennifer R
• Chen Bob
• Alford Spencer Caleb
• Dimov Ivan

Classifications

• B01J2219/00317

  Microwell devices, i.e. having large numbers of wells · CPC title

• C40B30/08

  by measuring catalytic activity · CPC title

• G01N33/5304

  Reaction vessels, e.g. agglutination plates (for solid-phase systems G01N33/543) · CPC title

• G01N33/54366

  Apparatus specially adapted for solid-phase testing · CPC title

• G01N33/502

  for testing non-proliferative effects · CPC title