Polymerase Variants and Uses Thereof
US-2016333327-A1 · Nov 17, 2016 · US
Polymerase variants and uses thereof
US10526588B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10526588-B2 |
| Application number | US-201615151364-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 10, 2016 |
| Priority date | May 14, 2015 |
| Publication date | Jan 7, 2020 |
| Grant date | Jan 7, 2020 |
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- Title
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- Abstract
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Abstract
Official abstract text for this publication.
Described herein is a variant pol6 polymerase having at least one mutation selected from H223, N224, Y225, H227, 1295, Y342, T343, I357, S360, L361, I363, S365, S366, Y367, P368, D417, E475, Y476, F478, K518, H527, T529, M531, N535, G539, P542, N545, Q546, A547, L549, I550, N552, G553, F558, A596, G603, A610, V615, Y622, C623, D624, I628, Y629, R632, N635, M641, A643, I644, T647, I648, T651, I652, K655, W656, D657, V658, H660, F662, L690 and combinations thereof.
First claim
Opening claim text (preview).
What is claimed is: 1. A modified DNA polymerase having a DNA polymerase activity comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 2, which amino acid sequence includes an amino acid substitution corresponding to H527Y/T/M, wherein said modified DNA polymerase has an altered characteristic compared to a parental polymerase. 2. The modified DNA polymerase of claim 1 , wherein the altered characteristic is selected from fidelity, processivity, elongation rate, stability, solubility and the ability to bind and/or incorporate polyphosphate nucleotides selected from a tetraphosphate, pentaphosphate, hexaphosphate, heptaphosphate or octophosphate nucleotide. 3. The modified DNA polymerase of claim 1 , wherein the altered characteristic is the ability to incorporate a nucleotide polyphosphate having 4, 5, 6, 7 or 8 phosphates into a growing DNA strand. 4. The modified DNA polymerase of claim 3 , wherein the nucleotide polyphosphate is tagged. 5. The modified DNA polymerase of claim 1 , wherein said polymerase comprises less than 10 mutations. 6. The modified DNA polymerase of claim 1 , wherein the parental polymerase has an amino acid sequence consisting of SEQ ID NO: 1. 7. The modified DNA polymerase of claim 1 , wherein the parental polymerase has an amino acid sequence consisting of SEQ ID NO: 2.
Assignees
Inventors
Classifications
DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase · CPC title
- C12N9/1252Primary
DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase · CPC title
Nucleotidyltransferases (2.7.7) · CPC title
- C12N9/1241Primary
Nucleotidyltransferases (2.7.7) · CPC title
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- What does patent US10526588B2 cover?
- Described herein is a variant pol6 polymerase having at least one mutation selected from H223, N224, Y225, H227, 1295, Y342, T343, I357, S360, L361, I363, S365, S366, Y367, P368, D417, E475, Y476, F478, K518, H527, T529, M531, N535, G539, P542, N545, Q546, A547, L549, I550, N552, G553, F558, A596, G603, A610, V615, Y622, C623, D624, I628, Y629, R632, N635, M641, A643, I644, T647, I648, T651, I6…
- Who is the assignee on this patent?
- Roche Sequencing Solutions Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N9/1252. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jan 07 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 5 related publications on this page (citations in our corpus or others sharing the same primary CPC).