Ligand functionalized polymers

Patent metadata

Field	Value
Publication number	US-10526366-B2
Application number	US-201815987441-A
Country	US
Kind code	B2
Filing date	May 23, 2018
Priority date	Mar 3, 2010
Publication date	Jan 7, 2020
Grant date	Jan 7, 2020

Abstract

Ligand functionalized substrates, methods of making ligand functionalized substrates, and methods of using functionalized substrates are disclosed.

First claim

The invention claimed is:

1. A complex comprising:
a) a water-soluble or water-dispersible aminopolymer functionalized with pendent or catenary guanidinyl groups; and
b) a neutral or negatively charged target biological species in a biological fluid,
wherein 2 to 10 mole percent of the available amino groups of the aminopolymer functionalized with guanidinyl groups are alkylated and/or acylated.

2. The complex of claim 1 wherein the complex is a precipitated solid.

3. The complex of claim 1 wherein the aminopolymer selected from the group consisting of polyethylenimine, polylysine, polyaminoamides, polyallylamine, polyvinylamine, polydimethylamine-epichlorohydrin-ethylenediamine, polyaminosiloxanes and dendrimers formed form polyamidoamine (PAMAM) and polypropylenimine.

4. The complex of claim 1 wherein the aminopolymer is polyethylenimine or polyallylamine.

5. The complex of claim 1 wherein the target biological species is cells or cell debris.

6. The complex of claim 1 wherein the target biological species protein or enzyme.

7. The complex of claim 1 wherein the target biological species comprises antibodies.

8. The complex of claim 2 wherein the salt concentration of the biological fluid is between 0 and 300 millimolar.

9. The complex of claim 2 wherein the salt concentration of fluid is between 150 to 300 millimolar.

10. The complex of claim 2 wherein the salt concentration of the fluid is at least 150 mM.

11. The complex of claim 1 wherein the amount of aminopolymer functionalized with guanidinyl groups relative to the amount of target biological species is 0.01% to 100% by weight.

12. The complex of claim 11 wherein the amount of aminopolymer functionalized with guanidinyl groups relative to the amount of target biological species is 0.1%-10% by weight.

13. The complex of claim 1 wherein the aminopolymer functionalized with guanidinyl groups is present in amounts of 0.01 to 5000 micrograms/mL of fluid.

14. The complex according to claim 1 , wherein the complex precipitates from the fluid.

15. The complex of claim 1 wherein at least some of the aminopolymer functionalized with guanidinyl groups is of the formula:

wherein
R 2 is a H, C 1 -C 12 alkyl, C 5 -C 12 (hetero)aryl, or a residue of the polymer chain;
each R 3 is independently H, C 1 -C 12 alkyl, or C 5 -C 12 (hetero)aryl, each R 4 is H, C 1 -C 12 alkyl or alkylene, C 5 -C 12 (hetero)aryl or (hetero)arylene, cyano, or —C(═NH)—N(R 2 )-Polymer, and
n is 1 or 2.

Assignees

• 3M Innovative Properties Co

Inventors

• Rasmussen Jerald K
• Seshadri Kannan
• Fitzsimons Jr Robert T
• Hembre James I
• Bothof Catherine A
• Satterwhite Erin A
• Griesgraber George W
• He Yi
• Haddad Louis C

Classifications

• G01N33/545

  Synthetic resin · CPC title

• G01N33/54393

  Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding · CPC title

• C08G73/022

  from polyamines and epihalohydrins · CPC title

• C08G73/0206

  Polyalkylene(poly)amines · CPC title

• C08G73/028

  Polyamidoamines · CPC title