Methods and apparatus for processing fluids

Patent metadata

Field	Value
Publication number	US-10525425-B2
Application number	US-201514878299-A
Country	US
Kind code	B2
Filing date	Oct 8, 2015
Priority date	Jan 7, 2011
Publication date	Jan 7, 2020
Grant date	Jan 7, 2020

Abstract

A method for processing a fluid includes removably securing a retention member to a vessel that bounds a chamber; inserting a collapsible bag within the chamber of the vessel; securing the bag to the retention member so that the bag is supported within the chamber of the vessel; and dispensing a fluid into a compartment of the collapsible bag supported within the chamber of the vessel. The fluid can be mixed within bag while the bag is disposed within the vessel.

First claim

What is claimed is:

1. A method for processing a fluid, the method comprising:
removably securing a retention member to a vessel that bounds a chamber so that the retention member is supported by the vessel, the retention member comprising a plurality of catches;
inserting a collapsible bag within the chamber of the vessel, the collapsible bag being comprised of one or more sheets of water impermeable polymeric material so that the collapsible bag is water impermeable, the bag bounding a compartment that is sealed closed from the outside environment, the collapsible bag having a plurality of alignment tabs;
securing the collapsible bag to the retention member by securing the alignment tabs to the catches so that the collapsible bag is attached to the retention member at a plurality of spaced apart locations and supported within the chamber of the vessel, the collapsible bag having the compartment that is sealed closed when the collapsible bag is secured to the retention member; and
dispensing a fluid into the compartment of the collapsible bag supported within the chamber of the vessel.

2. The method as recited in claim 1 , wherein the retention member comprises a ring body that at least partially encircles the vessel, the plurality of catches projecting from the ring body.

3. The method as recited in claim 1 , further comprising removing the retention member from the vessel and the bag while the bag remains within the chamber of the vessel.

4. The method as recited in claim 1 , further comprising mixing the fluid within the compartment of the collapsible bag.

5. The method as recited in claim 1 , wherein the vessel is removably positioned on a movable cart.

6. The method as recited in claim 5 , further comprising:
coupling the cart directly to a docking station, the docking station comprising a stand and a drive motor assembly mounted thereon; and
extending a drive shaft from the drive motor assembly to the bag.

7. A fluid processing system comprising:
a vessel that bounds a chamber;
a retention member removably secured to the vessel so that the retention member is supported by the vessel, the retention member comprising a plurality of catches; and
a collapsible bag comprising a plurality of alignment tabs and bounding a compartment that is sealed closed to the outside environment, the collapsible bag being positioned within the chamber of the vessel and the plurality of alignment tabs being removably secured to the plurality of catches so that the bag having the compartment that is sealed closed is attached to the retention member at a plurality of spaced apart locations and is supported within the chamber of the vessel, the compartment that is sealed closed being independent of the vessel, the bag being comprised of one or more sheets of water impermeable polymeric materials so that the bag is water impermeable.

8. The fluid processing system as recited in claim 7 , wherein the retention member comprises a ring body that at least partially encircles the vessel, the plurality of catches projecting from the ring body.

9. The fluid processing system as recited in claim 7 , further comprising an impeller disposed within the bag.

10. The fluid processing system recited in claim 7 , the retention member further comprising a notch recessed on a bottom edge of the retention member so that when the retention member is removably secured to the vessel, the notch exposes the vessel.

11. The method as recited in claim 1 , wherein dispensing a fluid into the compartment of the collapsible bag comprises dispensing a biological solution into the compartment of the collapsible bag.

12. The method as recited in claim 11 , wherein the biological solution is a cell culture.

13. The method as recited in claim 1 , wherein the collapsible bag includes a port in communication with the compartment and the fluid dispensed into the compartment of the collapsible bag is dispensed through the port.

14. The fluid processing system recited in claim 7 , wherein the collapsible bag includes a port in communication with the compartment that is sealed closed.

15. The fluid processing system recited in claim 7 , wherein the compartment that is sealed closed is sterile.

16. The method as recited in claim 1 , wherein the plurality of spaced apart locations are disposed around a perimeter of the vessel.

17. The method as recited in claim 16 , wherein each of the plurality of spaced apart locations is disposed at or near an upper edge of the vessel.

18. The method as recited in claim 1 , wherein:
the retention member further comprises a c-shaped ring body having two opposing ends; and
the step of removably securing the retention member to the vessel includes drawing the two opposing ends toward each other so that the c-shaped ring body is secured around a circumference of the vessel.

19. The fluid processing system of claim 7 , wherein the collapsible bag further comprises:
an upper end;
a lower end;
a side extending between the upper and lower ends; and
an upper perimeter edge formed between the upper end and the side.

20. The fluid processing system of claim 19 , wherein the upper perimeter edge of the collapsible bag is secured to the retention member.

21. The fluid processing system of claim 20 , wherein the retention member secures the upper perimeter edge of the collapsible bag at least partially around a circumference of the vessel.

22. A method for processing a fluid, the method comprising:
removably securing a retention member to a vessel that bounds a chamber so that the retention member is supported by the vessel, the retention member comprising a c-shaped ring body having two opposing ends, wherein removably securing the retention member to the vessel includes drawing the two opposing ends toward each other so that the retention member is secured around a circumference of the vessel;
inserting a collapsible bag within the chamber of the vessel, the collapsible bag being comprised of one or more sheets of water impermeable polymeric material so that the collapsible bag is water impermeable, the bag bounding a compartment that is sealed closed from the outside environment;
securing the collapsible bag to the retention member so that the collapsible bag is attached to the retention member at a plurality of spaced apart locations and is supported within the chamber of the vessel, the collapsible bag having the compartment that is sealed closed when the collapsible bag is secured to the retention member; and
dispensing a fluid into the compartment of the collapsible bag supported within the chamber of the vessel.

Assignees

• Life Technologies Corp

Inventors

• Goodwin Michael E
• Lee Jacob D
• Woods Whitt F
• Draper Patrick L

Classifications

• C12M23/26 Primary

  flexible (flexible containers for laboratory use B01L3/505) · CPC title

• C12M27/02

  Stirrer or mobile mixing elements · CPC title

• B65B3/045

  for filling flexible containers having a filling and dispensing spout, e.g. containers of the "bag-in-box"-type · CPC title

• C12M29/06

  Nozzles; Sprayers; Spargers; Diffusers (per se B01F23/231, B01J19/26) · CPC title

• B01F2215/0431

  Numerical size values, e.g. diameter of a hole or conduit, area, volume, length, width, or ratios thereof · CPC title