System, method and computer-accessible medium for multi-plane imaging of neural circuits

Patent metadata

Field	Value
Publication number	US-10520712-B2
Application number	US-201615741435-A
Country	US
Kind code	B2
Filing date	Jul 1, 2016
Priority date	Jul 1, 2015
Publication date	Dec 31, 2019
Grant date	Dec 31, 2019

Abstract

An exemplary device can be provided which can include, for example, a radiation source(s) configured to generate a first radiation(s), a spatial light modulator (SLM) arrangement(s) configured to receive the first radiation(s) and generate a second radiation(s) based on the first radiation(s), and a galvanometer(s) configured to receive the second radiation(s), generate a third radiation(s) based on the second radiation(s), and provide the third radiation(s) to a sample(s).

First claim

What is claimed is:

1. A device, comprising:
at least one radiation source configured to generate at least one first radiation;
at least one spatial light modulator (SLM) arrangement configured to receive the at least one first radiation and generate at least one second radiation based on the at least one first radiation, wherein the at least one SLM arrangement includes:
a SLM configured to generate the at least one second radiation;
a pre-SLM afocal telescope configured to resize the at least one first radiation to match an area of the SLM;
a plurality of folding mirrors configured to redirect the at least one first radiation to the pre-SLM afocal telescope; and
a post SLM afocal telescope configured to resize the at least one second radiation to match a size of an acceptance aperture of the at least one galvanometer; and

at least one galvanometer configured to receive the at least one second radiation, generate at least one third radiation based on the at least one second radiation, and provide the at least one third radiation to at least one sample.

2. The device of claim 1 , wherein the at least one galvanometer includes at least one resonant galvanometer.

3. The device of claim 1 , wherein the at least one SLM arrangement further includes at least one broadband waveplate located between the pre-SLM afocal telescope and the SLM.

4. The device of claim 3 , wherein the at least one broadband waveplate is configured to rotate a polarization of the at least one first radiation to cause the at least one first radiation to be parallel with an active axis of the SLM.

5. The device of claim 1 , wherein the SLM is configured to split the at least one first radiation into the radiation beamlets which are the at least one second radiation.

6. The device of claim 5 , wherein the at least one SLM arrangement is configured to independently dynamically control each of the radiation beamlets.

7. The device of claim 5 , wherein the SLM splits the at least one first radiation into the radiation beamlets by imprinting a phase profile across the at least one first radiation.

8. The device of claim 5 , wherein the at least one galvanometer is further configured to direct each of the radiation beamlets to at least one of (i) a different area or (ii) a different plane of the at least one sample.

9. The device of claim 1 , wherein the at least one radiation source is at least one laser source.

10. The device of claim 1 , further comprising at least one pocket cell located between the at least one radiation source and the at least one SLM arrangement.

11. The device of claim 10 , wherein the at least one pocket cell is configured to modulate an intensity of the at least one first radiation.

12. The device of claim 1 , further comprising a computer processing arrangement configured to generate at least one image of the at least one sample based on at least one fourth radiation received from the at least one sample that is based on the at least one third radiation.

13. The device of claim 1 , further comprising a computer processing arrangement configured to generate at least one image of the at least one sample based on a plurality of resultant radiations received from the at least one sample that are based on the radiation beamlets.

14. The device of claim 13 , wherein a first number of the resultant radiations is based on a second number of the radiation beamlets.

15. The device of claim 14 , wherein the second number of the radiation beamlets is based on a third number of the planes of the at least one sample.

16. The device of claim 15 , wherein the computer processing arrangement is further configured to generate a third number of images of the at least one sample based on the resultant radiations.

17. The device of claim 13 , wherein the at least one image includes a plurality of images.

18. The device of claim 17 , wherein the computer processing arrangement is further configured to generate at least one multiplane image based on the images.

19. The device of claim 18 , wherein the at least one multiplane image is generated by interleaving the images into the at least one multiplane image.

20. The device of claim 18 , wherein the computer processing arrangement is further configured to correct brain motion artifacts in the images based on a pyramid procedure.

21. A method, comprising:
generating at least one radiation;
directing the at least one radiation to a pre-spatial light modulator (SLM) afocal telescope using a plurality of folding mirrors;
resizing the at least one redirected radiation using the pre-SLM afocal telescope to match an area of at least one (SLM);
splitting the at least one resized radiation into a plurality of radiation beamlets using the at least one SLM;
resizing the radiation beamlets to match a size of an acceptance aperture of at least one galvanometer; and
directing the radiation beamlets to at least one sample using the at least one galvanometer.

Assignees

• Univ Columbia

Inventors

• Yuste Rafael
• Peterka Darcy S
• Yang Weijian

Classifications

• G02B21/367

  providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison · CPC title

• G02F1/03

  based on ceramics or electro-optical crystals, e.g. exhibiting Pockels effect or Kerr effect (G02F1/061 takes precedence) · CPC title

• G02B2207/114

  Two photon or multiphoton effect · CPC title

• G02B21/06

  Means for illuminating specimens · CPC title

• G06T2207/30016

  Brain · CPC title