Method for selectively producing ginsenoside Rd from saponins of ginseng through enzymatic method

Patent metadata

Field	Value
Publication number	US-10519480-B2
Application number	US-201615769995-A
Country	US
Kind code	B2
Filing date	Oct 21, 2016
Priority date	Oct 22, 2015
Publication date	Dec 31, 2019
Grant date	Dec 31, 2019

Abstract

The present invention relates to a method for selectively producing ginsenoside Rd, which is originally present in ginseng in a trace amount, from panaxadiol-type saponins of ginseng, and more specifically to a method capable of obtaining a desired target compound, that is, ginsenoside Rd, in high yields, by treating a panaxadiol-type saponin obtained from ginseng, with particular enzymes to structurally convert the saponins.

First claim

The invention claimed is:

1. A method for producing ginsenoside Rd of the following chemical formula 1 from ginsenoside Rb1,
said method comprising contacting a substrate comprising ginsenoside Rb1 with pectinase obtained from Trichoderma reesei thereby converting the ginsenoside Rb1 to ginsenoside Rd:

2. The method according to claim 1 , wherein the substrate comprising ginsenoside Rb1 is a ginseng saponin.

3. The method according to claim 2 , wherein the method comprises the steps of:
1) contacting the ginseng saponin in an aqueous solvent or a mixed solution of an aqueous solvent and an organic solvent with the pectinase, and allowing the converting undergoes thereby producing a reaction mixture comprising ginsenoside Rd as a conversion product;
2) inactivating the pectinase in the reaction mixture of 1) thereby completing the conversion reaction; and
3) adding ethyl acetate or ethanol to the reaction mixture obtained in 2), followed by extraction and concentration, thereby isolating ginsenoside Rd.

4. The method according to claim 1 , wherein the pectinase is added in an amount of 10 to 400% by weight based on the amount of the substrate.

5. The method according to claim 1 , wherein the converting of 1) is carried out at a temperature of 35 to 60° C.

6. The method according to claim 3 , wherein the converting of 1) is carried out for 1 to 48 hours.

7. The method according to claim 3 , wherein the aqueous solvent or the mixed solution of an aqueous solvent and an organic solvent has a pH in the range of 3.5 to 5.5.

Assignees

• Amorepacific Corp

Inventors

• Nam Gi Baeg
• Kim Dong Hyun
• Hong Yong Deog
• Park Jun Seong

Classifications

• C07H17/00

  Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals · CPC title

• C12P19/44

  Preparation of O-glycosides, e.g. glucosides {(polysaccharides and not substituted disaccharides C12P19/04, C12P19/12)} · CPC title

• C07J17/00

  Normal steroids containing carbon, hydrogen, halogen or oxygen, having an oxygen-containing hetero ring not condensed with the cyclopenta(a)hydrophenanthrene skeleton (cardanolide, bufanolide C07J19/00) · CPC title

• C12P33/16 Primary

  Acting at 17 position · CPC title

• C12Y302/01004

  Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase · CPC title