Shaft-mounted fluid transfer assembly for a disposable bioreactor

Patent metadata

Field	Value
Publication number	US-10519409-B2
Application number	US-201515534633-A
Country	US
Kind code	B2
Filing date	Dec 22, 2015
Priority date	Dec 31, 2014
Publication date	Dec 31, 2019
Grant date	Dec 31, 2019

Abstract

A fluid transfer assembly for single use bioreactors includes a fluid transfer housing that can be mounted to the impeller shaft using a bearing that places the fluid transfer assembly directly below the lowest impeller but allows the impeller shaft to spin inside independently of the fluid transfer assembly. A fluid conduit connects the fluid transfer housing to a port in the single use bag wall which allows fluids to be introduced into the sparger and which also helps prevent the fluid transfer assembly from rotating with the impeller shaft.

First claim

What is claimed is:

1. A fluid transfer assembly for a disposable bioreactor comprising:
a fluid transfer housing having a housing body defining an input port, at least one outlet port and an elongate housing passageway extending in fluid communication therebetween;
said housing body further defining an open shaft passageway extending therethrough; and
a rolling element bearing affixed within said shaft passageway, said rolling element bearing comprising concentric inner and outer bearing races and a plurality of rolling elements positioned between the inner and outer bearing races, the outer bearing race being affixed to the housing body and the inner bearing race including an inner surface defining a shaft aperture such that the inner race is affixable to an impeller shaft so that the shaft may rotate independently of said housing body.

2. The fluid transfer assembly of claim 1 , further comprising a plurality of outlet ports in fluid communication with said housing passageway.

3. The fluid transfer assembly of claim 1 , wherein said housing body includes opposed first and second planar major surfaces and a perimetrical side wall extending between said first and second major surfaces.

4. The fluid transfer assembly of claim 3 , wherein said input port is defined by said perimetrical side wall of said housing.

5. The fluid transfer assembly of claim 3 , wherein said first major surface of said housing body defines said at least one outlet port.

6. The fluid transfer assembly of claim 5 , wherein said first major surface defines a plurality of outlet ports in fluid communication with said housing passageway.

7. The fluid transfer assembly of claim 3 , wherein said housing passageway is defined to extend along the perimetrical side wall about said shaft aperture.

8. The fluid transfer assembly of claim 7 , wherein said first major surface of said housing body defines a plurality of outlet ports in fluid communication with said housing passageway, wherein said plurality of outlet ports are arrayed about said shaft aperture.

9. The fluid transfer assembly of claim 3 , further comprising a porous member spanning said outlet aperture.

10. The fluid transfer assembly of claim 9 , wherein the pore size of the porous member is from about 2 micrometers to about 10 millimeters.

11. The fluid transfer assembly of claim 9 , wherein the pore size of the porous member is from about 2 micrometers to about 3 millimeters.

12. The fluid transfer assembly of claim 9 , wherein the pore size of the porous member is from about 5 micrometers to about 3 millimeters.

13. The fluid transfer assembly of claim 9 , wherein the porous member associated with the outlet port is also adapted for use as a particulate separation device.

14. The fluid transfer assembly of claim 1 , wherein said inlet port is further defined by an elongate port stem projecting from said housing body, said port stem defining an open elongate port passageway in open fluid communication with said housing passageway.

15. The fluid transfer assembly of claim 1 , wherein said housing body further defines at least one open reactor passageway through said housing body, said reactor passageway positioned adjacent to said shaft passageway.

16. The fluid transfer assembly of claim 1 , wherein said housing body has a general shape selected from ring-shaped, square-shaped, and polygonal-shaped.

17. The fluid transfer assembly of claim 3 , wherein said perimetrical side wall has a shape selected from rounded cylindrical and polygonal cylindrical.

18. The fluid transfer assembly of claim 1 , further comprising an elongate fluid conduit extending from said input port of said fluid transfer housing, said fluid conduit having opposed first and second ends and an elongate tubular conduit body extending therebetween, said first end of said fluid conduit defining a first conduit aperture, said second end of said fluid conduit defining a second conduit aperture, and said conduit body defining an elongate conduit passageway extending in open fluid communication therebetween, said fluid conduit for placing said outlet port of said fluid transfer housing in open fluid communication with a fluid port of a container wall, such that fluid may be provided between the fluid port of the container wall and a bioreactor cavity defined by the container through said fluid transfer housing.

19. An impeller assembly for a disposable bioreactor, said impeller assembly comprising:
an elongate impeller shaft having opposed first and second ends and an elongate shaft body extending therebetween;
at least one impeller hub comprising a plurality of impeller blades, said hub mounted to said impeller shaft;
the fluid transfer assembly of claim 1 , the fluid transfer housing of said fluid transfer assembly being rotatably mounted to said impeller shaft, such that said impeller shaft extends through said shaft passageway of said housing body.

20. The impeller assembly of claim 19 , wherein the inner race of said bearing is affixed to said impeller shaft.

21. The impeller assembly of claim 19 , comprising a second fluid transfer assembly of any one of claims 1 - 18 , the fluid transfer housing of said second fluid transfer assembly being rotatably mounted to said impeller shaft, such that said impeller shaft extends through said shaft passageway of said housing body, said second fluid transfer assembly mounted opposite said at least one impeller hub from said first fluid transfer assembly.

22. The impeller assembly of claim 19 , further comprising a second impeller hub comprising a plurality of impeller blades, said second impeller hub mounted to said impeller shaft at a location opposite said second fluid transfer housing from said at least one impeller hub.

23. A disposable bioreactor comprising:
a flexible bioreactor container having a flexible container wall defining a bioreactor cavity;
the impeller assembly of claim 19 provided in said bioreactor cavity;
a fluid port mounted to said container wall, said fluid port defining an open aperture through said container wall;
an elongate fluid conduit extending from said fluid port of said bioreactor wall to said input port of said fluid transfer housing, said fluid conduit having opposed first and second ends and an elongate tubular conduit body extending therebetween, said first end of said fluid conduit defining a first conduit aperture, said second end of said fluid conduit defining a second conduit aperture, and said conduit body defining an elongate conduit passageway extending in open fluid communication therebetween, said fluid conduit placing said outlet port of said fluid transfer housing in open fluid communication with said fluid port of said container wall, such that fluid may be provided between said fluid port of said container wall and said bioreactor cavity through said fluid transfer housing.

24. The fluid transfer assembly of claim 1 , wherein the plurality of rolling elements are balls or rollers.

Assignees

• Ge Healthcare Bio Sciences Corp

Inventors

• Damren Richard Lee
• Tuohey Colin R

Classifications

• C12M23/28

  disposable or single use · CPC title

• C12M29/06

  Nozzles; Sprayers; Spargers; Diffusers (per se B01F23/231, B01J19/26) · CPC title

• C12M27/04

  with introduction of gas through the stirrer or mixing element · CPC title

• C12M23/14 Primary

  Bags · CPC title

• C12M1/04

  with gas introduction means · CPC title