Biosensor structures for improved point of care testing and methods of manufacture thereof

Patent metadata

Field	Value
Publication number	US-10514354-B2
Application number	US-201615285687-A
Country	US
Kind code	B2
Filing date	Oct 5, 2016
Priority date	Mar 15, 2013
Publication date	Dec 24, 2019
Grant date	Dec 24, 2019

Abstract

The present invention relates to analytical testing devices and methods for fabricating electrochemical creatinine biosensors, and in particular using point of care electrochemical biosensors for testing for creatinine in samples. For example, the present invention may be directed to a biosensor having an electrode, a first printed layer formed on the electrode and having a first matrix that includes creatinine amidohydrolase (CNH), creatine amidinohydrolase (CRH), and sarcosine oxidase (SOX), and second printed layer formed over the first printed layer and having a second matrix that includes CRH, SOX, and catalase.

First claim

We claim:

1. A method of manufacturing a biosensor comprising:
forming an electrode on a wafer;
microdispensing a first matrix that includes creatinine amidohydrolase (CNH), creatine amidinohydrolase (CRH), and sarcosine oxidase (SOX) to form a first layer on the electrode;
microdispensing a second matrix that includes CRH to form a second layer over the first layer; and
microdispensing a third matrix that includes CRH, SOX, and catalase to form a third layer over the second layer such that the second layer is disposed between the first layer and the third layer,
wherein the second layer completely covers the first layer,
wherein the third layer completely covers the second layer, and
wherein the second matrix and the third matrix are different.

2. The method of claim 1 , wherein the first layer and the third layer are at least one of microdispensed and dried at a controlled humidity in a range of about 40-98% relative humidity.

3. The method of claim 1 , further comprising, prior to forming the first layer, the second layer, and the third layer, forming a silane layer on the electrode such that the silane layer is disposed between the electrode and the first player.

4. The method of claim 1 , further comprising, prior to forming the first layer, the second layer, and the third layer, forming a gamma amino silane layer on the electrode such that the gamma amino silane layer is disposed between the electrode and the first layer.

5. The method of claim 1 , wherein a diameter of the electrode is less than a diameter of the first layer and a diameter of the second layer.

6. The method of claim 1 , wherein a diameter of the first layer is less than or equal to a diameter of the second layer.

7. The method of claim 1 , wherein the first matrix and the second third matrix are polymer matrixes.

8. The method of claim 1 , wherein the first matrix and the third matrix are selected from the group consisting of: polyvinyl alcohol, gelatin, acrylamide, polyethyleneglycol diacrylate, or combinations thereof.

9. The method of claim 1 , wherein the first matrix and the third matrix are photoformable.

10. The method of claim 1 , wherein the first matrix and the third matrix comprise a photoinitiator.

11. The method of claim 10 , wherein the photoinitiator is stilbazonium or dichromate.

12. The method of claim 1 , wherein the first layer, the second layer, and the third layer comprise a substantially concave shape.

13. The method of claim 1 , wherein the first layer, the second layer, and the third layer comprise a substantially convex shape.

14. A method of manufacturing a biosensor comprising:
forming an electrode on a wafer;
printing a first layer on a top surface the electrode, the first layer comprising creatinine amidohydrolase (CNH), creatine amidinohydrolase (CRH), and sarcosine oxidase (SOX);
printing a second layer on a top surface of the first layer, the second layer comprising CRH and having a composition different from that of the first layer; and
printing a third layer on a top surface of the second layer such that the second layer is disposed between the first layer and the third layer, the third layer comprising CRH, SOX, and catalase and having a composition different from the second layer.

Assignees

• Abbott Point Of Care Inc

Inventors

• Zhao Tian-Xian
• Martin Glenn
• Hardage Kenneth
• Breeze Steven R
• Snyder Stephen

Classifications

• C25D5/48

  After-treatment of electroplated surfaces · CPC title

• G01N27/3271 Primary

  Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood (amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001) · CPC title

• C12Q1/005

  involving specific analytes or enzymes (including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence) · CPC title

• C23C14/58

  After-treatment · CPC title

• C23C14/34

  Sputtering · CPC title