Peptide-based nanofibrillar materials

Patent metadata

Field	Value
Publication number	US-10513802-B2
Application number	US-201415034983-A
Country	US
Kind code	B2
Filing date	Nov 7, 2014
Priority date	Nov 8, 2013
Publication date	Dec 24, 2019
Grant date	Dec 24, 2019

Abstract

Disclosed herein are nanofibrillar materials and aerogel-like materials comprised of nanofibrils, and methods for making such materials.

First claim

What is claimed is:

1. A nanofibrillar material, comprising:
nanofibrils formed from an amphiphilic polypeptide, wherein the nanofibrils have an average diameter between about 2 nm and about 40 nm and form a network of interconnected pores,
wherein a majority of the interconnected pores are nano-sized pores that range in diameter between about 2 nm and about 100 nm, and wherein at least 50% of the nano-sized pores are in the mesoporous range in diameter between 2 nm and 50 nm; and
wherein the nanofibrillar material is characterized by a porosity of at least about 80%, a bulk density that is less than about 75 kg/m 3 , and
a compressive modulus between that is between about 50 kPa and about 300 kPa.

2. The nanofibrillar material of claim 1 , wherein the amphiphilic polypeptide comprises a beta-sheet secondary structure.

3. The nanofibrillar material of claim 1 , wherein the amphiphilic polypeptide comprises an amino acid sequence of polypeptides selected from the group consisting of:
actins, catenins, claudins, coilins, collagens, elastins, elaunins, extensins, fibrillins, fibroins, keratins, laminins, lamins, tublins, viral structural proteins, zein proteins (seed storage protein) or combination thereof.

4. The nanofibrillar material of claim 1 , wherein the amphiphilic polypeptide comprises a silk fibroin polypeptide.

5. The nanofibrillar material of claim 1 , wherein the amphiphilic polypeptide has an average molecular weight between 30 kDa and about 400 kDa.

6. The nanofibrillar material of claim 4 , wherein the silk fibroin polypeptide comprises a wild type amino acid sequence of Bombyx mori fibroin.

7. The nanofibrillar material of claim 4 , wherein the silk fibroin polypeptide comprises a variant of Bombyx mori fibroin.

8. The nanofibrillar material of claim 4 , wherein the silk fibroin polypeptide is a low molecular weight silk fibroin polypeptide having a molecular weight between 30 kDa and about 125 kDa.

9. The nanofibrillar material of claim 1 , wherein the nanofibrillar material is characterized by a porosity of at least about 90%.

10. The nanofibrillar material of claim 1 , further comprising platinum(II) meso-tetrakis(pentafluorophenyl)porphyrin (PtTFPP) for oxygen sensing.

11. The nanofibrillar material of claim 1 , further comprising an additive.

12. The nanofibrillar material of claim 11 , wherein the additive is or comprises one or more a therapeutic agent, a preventative agent, and a diagnostic agent.

Assignees

• Univ Tufts

Inventors

• Omenetto Fiorenzo G
• Kaplan David L
• Marelli Benedetto
• Mitropoulos Alexander Nicholas

Classifications

• C07K14/78

  Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG] · CPC title

• C08J9/28

  by elimination of a liquid phase from a macromolecular composition or article, e.g. drying of coagulum · CPC title

• A61L27/3604

  characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel · CPC title

• A61L27/227

  Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24 · CPC title

• C12Q1/26

  involving oxidoreductase · CPC title