Marker associated with resistance to smut in plant belonging to genus Saccharum, and use thereof

Patent metadata

Field	Value
Publication number	US-10513744-B2
Application number	US-201715664093-A
Country	US
Kind code	B2
Filing date	Jul 31, 2017
Priority date	Apr 28, 2011
Publication date	Dec 24, 2019
Grant date	Dec 24, 2019

Abstract

The present invention relates to a marker associated with resistance to smut which is a quantitative trait of sugarcane. Specifically, a marker associated with resistance to sugarcane smut, which consists of a continuous nucleic acid region existing in a region sandwiched between the nucleotide sequence shown in SEQ ID NO: 1 and the nucleotide sequence shown in SEQ ID NO: 14 or a different similar region, is provided.

First claim

The invention claimed is:

1. A method for producing a sugarcane line having improved smut resistance, said method comprising:
extracting genomic DNA from a progeny plant obtained from parent plants, at least one of which is a sugarcane plant, and/or a genomic DNA of a parent sugarcane plant;
determining by nucleic acid assay the presence of a marker associated with resistance to sugarcane smut in the obtained genomic DNA, and selecting the progeny plant as a plant having improved smut resistance based on the presence of said marker; and
using the selected plant as a parent plant for crossing, to thereby produce progeny plant(s),
wherein said marker consists of a continuous nucleic acid region in the obtained genomic DNA, and wherein said marker comprises at least 20 continuous nucleotides of a nucleotide sequence selected from the group consisting of SEQ ID NOs: 19 to 22.

2. The method for producing a sugarcane line according to claim 1 , wherein a DNA chip that comprises a probe corresponding to the marker associated with resistance to sugarcane smut is used in the determination step.

3. The method for producing a sugarcane line according to claim 1 , wherein the progeny plant used in said genomic DNA extracting step is in the form of seeds or a young seedling, and the genomic DNA is extracted from the seeds or the young seedling.

4. The method for producing a sugarcane line according to claim 1 , wherein the progeny plant(s) produced using the selected plant as a parent plant for crossing is in the form of seeds or a young seedling.

Assignees

• Toyota Motor Co Ltd

Inventors

• Enoki Hiroyuki
• Kimura Tatsuro
• Nishimura Satoru
• Murakami Aya
• Terauchi Takayoshi
• Sakaigaichi Takeo
• Hattori Taiichiro
• Ishikawa Shoko
• Terajima Yoshifumi

Classifications

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12Q1/6895 Primary

  for plants, fungi or algae · CPC title

• C12Q2600/13

  Plant traits · CPC title

• A01H5/04

  Stems · CPC title

• A01H1/04

  Processes of selection {involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection} · CPC title