Glucosyltransferase enzymes for production of glucan polymers
US-9296996-B2 · Mar 29, 2016 · US
Modified glucosyltransferases for producing branched alpha-glucan polymers
US10508268B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10508268-B2 |
| Application number | US-201815955923-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 18, 2018 |
| Priority date | Jun 17, 2015 |
| Publication date | Dec 17, 2019 |
| Grant date | Dec 17, 2019 |
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- Title
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Abstract
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Glucosyltransferase enzymes are disclosed herein that produce branched alpha-glucan polymer. Also disclosed, for example, are polynucleotides encoding these enzymes, as well as methods of producing branched alpha-glucan polymer.
First claim
Opening claim text (preview).
What is claimed is: 1. A glucosyltransferase enzyme comprising an amino acid sequence that is at least 97% identical to: SEQ ID NO:85, SEQ ID NO:89, or SEQ ID NO:93, wherein said glucosyltransferase enzyme lacks the amino acid sequence of SEQ ID NO:78 or SEQ ID NO:80, and wherein said glucosyltransferase enzyme produces a branched alpha-glucan polymer. 2. The glucosyltransferase enzyme of claim 1 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 98% identical to SEQ ID NO:85, SEQ ID NO:89, or SEQ ID NO:93. 3. A reaction solution comprising water, sucrose, and a glucosyltransferase enzyme according to claim 1 . 4. A method for producing a branched alpha-glucan polymer comprising: (a) contacting at least water, sucrose, and a glucosyltransferase enzyme according to claim 1 , whereby branched alpha-glucan polymer is produced, and (b) optionally, isolating the branched alpha-glucan polymer produced in step (a). 5. The glucosyltransferase enzyme of claim 1 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 97% identical to SEQ ID NO:85. 6. The glucosyltransferase enzyme of claim 1 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 98% identical to SEQ ID NO:85. 7. The glucosyltransferase enzyme of claim 1 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 97% identical to SEQ ID NO:89. 8. The glucosyltransferase enzyme of claim 1 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 98% identical to SEQ ID NO:89. 9. The glucosyltransferase enzyme of claim 1 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 97% identical to SEQ ID NO:93. 10. The glucosyltransferase enzyme of claim 1 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 98% identical to SEQ ID NO:93.
Assignees
Inventors
Classifications
Mutagenizing nucleic acids · CPC title
DNA beta-glucosyltransferase (2.4.1.27) · CPC title
- C12N9/1051Primary
Hexosyltransferases (2.4.1) · CPC title
alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof · CPC title
Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds · CPC title
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Frequently asked questions
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- What does patent US10508268B2 cover?
- Glucosyltransferase enzymes are disclosed herein that produce branched alpha-glucan polymer. Also disclosed, for example, are polynucleotides encoding these enzymes, as well as methods of producing branched alpha-glucan polymer.
- Who is the assignee on this patent?
- Dupont Ind Biosciences Usa Llc
- What technology area does this patent fall under?
- Primary CPC classification C12N9/1051. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Dec 17 2019 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 9 related publications on this page (citations in our corpus or others sharing the same primary CPC).