Oral care products and methods comprising AFPs

Patent metadata

Field	Value
Publication number	US-10507340-B2
Application number	US-201615770978-A
Country	US
Kind code	B2
Filing date	Oct 25, 2016
Priority date	Oct 26, 2015
Publication date	Dec 17, 2019
Grant date	Dec 17, 2019

Abstract

Provided herein are oral care compositions comprising antifreeze proteins (AFPs) useful in methods of repairing or inhibiting dental erosion, promoting dental remineralization, and/or enhancing the anti-cavity effects of fluoride.

First claim

The invention claimed is:

1. A method of repairing or inhibiting or protection against or prevention of dental erosion, promoting dental remineralization, and/or enhancing the anti-cavity effects of fluoride comprising applying to the teeth an oral care composition comprising:
a) an antifreeze protein (AFP); and
b) an orally acceptable carrier,
wherein AFP has the polypeptide sequence as set forth in SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3, or a polypeptide sequence which arise from substitution, insertion or deletion of up to 5% of all amino acids, starting from the polypeptide sequence as set forth in SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3, and which still has at least 50% of the biological property of the starting protein, and wherein AFP is present in the composition in an amount of from 0.01 weight % to 3 weight % by total weight of the composition.

2. The method of claim 1 wherein the AFP is coupled to a hydroxyapatite binding peptide which is selected from the group formed from SEQ ID NO:4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, and 19.

3. The method of claim 1 wherein the AFP is a homomer or heteromer of AFPs and coupled to a hydroxyapatite binding peptide which is selected from the group formed from SEQ ID NO:4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, and 19.

4. The method described in claim 1 wherein the oral care composition further comprises an effective amount of fluoride.

5. The method of claim 1 wherein the composition is a dentifrice.

6. The method of claim 1 , wherein AFP has the polypeptide sequence as set forth in SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3, or a polypeptide sequence which arise from substitution, insertion or deletion of up to 3% of all amino acids, starting from the polypeptide sequence as set forth in SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3 and which still has at least 80% of the biological property of the starting protein, and wherein AFP is present in the composition in an amount of from 0.01 weight % to 3 weight % by total weight of the composition.

7. The method of claim 1 , wherein AFP has the polypeptide sequence as set forth in SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3, or a polypeptide sequence which arise from substitution, insertion or deletion of up to 1% of all amino acids, starting from the polypeptide sequence as set forth in SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3 and which still has at least 90% of the biological property of the starting protein, and wherein AFP is present in the composition in an amount of from 0.01 weight % to 3 weight % by total weight of the composition.

8. The method of claim 1 , wherein the AFP has the polypeptide sequence of SEQ ID NO: 1.

9. The method of claim 1 , wherein the AFP has the polypeptide sequence of SEQ ID NO: 2.

10. The method of claim 1 , wherein the AFP has the polypeptide sequence of SEQ ID NO: 3.

Assignees

• Basf Se

Inventors

• Schneider Nina
• Subkowski Thomas
• Jenewein Stefan
• Karos Marvin
• Bollschweiler Claus
• Wendel Volker
• Qiu Jianhong
• Kilpatrick-Liverman Latonya
• Zaidel Lynette

Classifications

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• A61P1/02

  Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis · CPC title

• A61K8/21

  Fluorides; Derivatives thereof · CPC title

• A61Q11/00 Primary

  Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses · CPC title

• A61K9/0058

  Chewing gums (non-medicinal aspects, preparing chewing gum A23G4/00; chewing gum for care of the teeth or oral cavity, e.g. with breath freshener A61Q11/00) · CPC title