Epitope of hepatitis B virus surface antigen and binding molecule specifically binding to same for neutralizing hepatitis B virus

Patent metadata

Field	Value
Publication number	US-10507240-B2
Application number	US-201515529828-A
Country	US
Kind code	B2
Filing date	Nov 27, 2015
Priority date	Nov 28, 2014
Publication date	Dec 17, 2019
Grant date	Dec 17, 2019

Abstract

The present invention relates to an epitope specific to hepatitis B virus surface antigen and a binding molecule binding to the same for neutralizing hepatitis B virus. Since the epitope provided by the present invention is produced by forming a three-dimensional structure and does not comprise a determinant, by which escape mutation is induced against an administration of existing vaccines or HBIg, a composition comprising an antibody biding to the epitope or a vaccine composition comprising the epitope has a very low possibility of causing a decrease in efficacy due to escape mutation. Therefore, such an antibody or vaccine composition can be very effectively used in prevention and/or treatment of HBV.

First claim

The invention claimed is:

1. A binding molecule for neutralizing hepatitis B virus (HBV), which specifically binds to an epitope that includes the amino acids at positions 110, 118 and 120 of a hepatitis B virus surface antigen (HBsAg), the amino acid sequence of which antigen is set forth in SEQ ID NO:1, wherein the binding molecule is a human monoclonal antibody or a fragment of such an antibody which comprises a polypeptide sequence comprising the CDR regions of SEQ ID NOS: 5 to 10 or the CDR regions of SEQ ID NOS: 11 to 16.

2. The binding molecule of claim 1 , wherein the epitope further includes an amino acid at position 147.

3. The binding molecule of claim 1 , wherein the binding molecule has a binding affinity of less than 1×10 −9 M.

Assignees

• Celltrion Inc

Inventors

• Yoon Joon Sun
• Lee Hwa Jin
• Yi Kye Sook
• Kim Cheol Min
• Lim Byung Pil
• Chang Shin Jae
• Hong Seung Suh

Classifications

• A61P37/04

  Immunostimulants · CPC title

• A61P31/20

  for DNA viruses · CPC title

• C07K16/082 Primary

  Hepadnaviridae (F), e.g. hepatitis B virus · CPC title

• C07K16/08

  against material from viruses · CPC title

• C07K14/02

  Hepadnaviridae, e.g. hepatitis B virus · CPC title