Methods for production of oxygenated terpenes

Patent metadata

Field	Value
Publication number	US-10501760-B2
Application number	US-201515505022-A
Country	US
Kind code	B2
Filing date	Aug 21, 2015
Priority date	Aug 21, 2014
Publication date	Dec 10, 2019
Grant date	Dec 10, 2019

Abstract

The present disclosure relates to methods for producing oxygenated terpenoids, and preparation of compositions and formulations thereof. Polynucleotides, derivative enzymes, and host cells for use in such methods are also provided.

First claim

The invention claimed is:

1. A formulation, comprising:
nootkatone, wherein the nootkatone is present in the formulation in an amount ranging from about 55% to about 60% (w/w);
α-nootkatol, wherein the α-nootkatol is present in the formulation in an amount ranging from about 20% to about 25% (w/w); and
β-nootkatol, wherein the β-nootkatol is present in the formulation in an amount ranging from about 5% to about 10% (w/w).

2. The formulation of claim 1 , wherein at least one of the nootkatone, the α-nootkatol, and the β-nootkatol, is produced using a biosynthetic process.

3. The formulation of claim 1 , wherein the nootkatone, the α-nootkatol, and the β-nootkatol, are produced using at least one biosynthetic process.

4. The formulation of claim 1 , wherein the formulation is a natural flavor formulation.

5. The formulation of claim 1 , further comprising one or more non-sesquiterpene components or sesquiterpene components.

6. The formulation of claim 5 , wherein the one or more non-sesquiterpene components or sesquiterpene components is selected from Table 8A, products obtained from oxidation of valencene by the cytochrome P450 enzyme Stevia rebaudiana Kaurene Oxidase (SrKO).

7. The formulation of claim 1 , further comprising valencene.

8. The formulation of claim 7 , wherein the valencene is present in the formulation in an amount ranging from about 5% to about 10% (w/w).

9. The formulation of claim 7 , wherein at least one of the nootkatone, the α-nootkatol, the β-nootkatol, and the valencene is produced using a biosynthetic process.

10. The formulation of claim 7 , wherein the nootkatone, the α-nootkatol, the β-nootkatol, and the valencene are produced using at least one biosynthetic process.

11. The formulation of claim 8 , wherein the formulation is a natural flavor formulation.

12. The formulation of claim 8 , further comprising one or more non-sesquiterpene components or sesquiterpene components.

13. The formulation of claim 12 , wherein the one or more non-sesquiterpene components or sesquiterpene components is selected from products obtained from oxidation of valencene by the cytochrome P450 enzyme SrKO.

Assignees

• Givaudan Sa
• Manus Biosynthesis Inc

Inventors

• Kumaran Ajikumar Parayil
• Lim Chin Giaw
• Li Liwei
• Ghosh Souvik
• Pirie Christopher
• Qualley Anthony
• Marshall-Hill Geoff
• Preininger Martin

Classifications

• C12P7/26 Primary

  Ketones · CPC title

• C12N9/0073

  with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13 · CPC title

• C12P5/002

  cyclic (compounds containing at least three condensed carbocyclic rings C12P15/00) · CPC title

• A23L27/13

  from citrus fruits · CPC title

• C07K2319/03

  containing a transmembrane segment · CPC title