Herbicide tolerant cotton event pDAB4468.19.10.3

Patent metadata

Field	Value
Publication number	US-10501752-B2
Application number	US-201715452422-A
Country	US
Kind code	B2
Filing date	Mar 7, 2017
Priority date	Jan 23, 2012
Publication date	Dec 10, 2019
Grant date	Dec 10, 2019

Abstract

Cotton event pDAB4468.19.10.3 comprises genes encoding AAD-12 and PAT, affording herbicide tolerance to cotton crops containing the event, and enabling methods for crop protection.

First claim

What is claimed is:

1. A transgenic cotton seed comprising in its genome a pat/aad-12 transgenic event comprising a polynucleotide that is at least 99% identical to SEQ ID NO:21.

2. The transgenic cotton seed of claim 1 , wherein the polynucleotide comprises a first nucleotide sequence consisting of nucleotides 1329-1380 of SEQ ID NO:1, and a second nucleotide sequence consisting of nucleotides 143-194 of SEQ ID NO:2.

3. The transgenic cotton seed of claim 2 , wherein the polynucleotide comprises a first nucleotide sequence consisting of nucleotides 1254-1455 of SEQ ID NO:1, and a second nucleotide sequence consisting of nucleotides 118-219 of SEQ ID NO:2.

4. The transgenic cotton seed of claim 3 , wherein the polynucleotide comprises a first nucleotide sequence consisting of nucleotides 1304-1405 of SEQ ID NO:1, and a second nucleotide sequence consisting of nucleotides 68-269 of SEQ ID NO:2.

5. The transgenic cotton seed of claim 4 , wherein the polynucleotide comprises a first nucleotide sequence consisting of nucleotides 1054-1655 of SEQ ID NO:1, and a second nucleotide sequence consisting of nucleotides 1-369 of SEQ ID NO:2.

6. The transgenic cotton seed of claim 1 , wherein the polynucleotide comprises SEQ ID NO:21.

7. A transgenic cotton plant comprising in its genome a pat/aad-12 transgenic event comprising a polynucleotide that is at least 99% identical to SEQ ID NO:21.

8. A part of the transgenic cotton plant of claim 7 , wherein the part is selected from the group consisting of pollen, ovule, flowers, bolls, shoots, roots, and leaves.

9. A method for breeding a cotton plant, the method comprising:
crossing the transgenic cotton plant of claim 7 with a second cotton plant to produce a progeny cotton plant; and
assaying the progeny cotton plant for the presence of the pat/aad-12 transgenic event.

10. The method according to claim 9 , wherein assaying the progeny cotton plant for the presence of the pat/aad-12 transgenic event comprises amplifying genomic DNA from the progeny plant in a polymerase chain reaction or applying an herbicide to the progeny cotton plant, wherein the herbicide is a phenoxyacetic acid herbicide, a pyridoxyacetic acid herbicide, or glufosinate.

11. A cotton meal, cotton fiber, or cotton oil from a genetically-modified plant, the meal, fiber, or oil comprising a polynucleotide that is at least 99% identical to SEQ ID NO:21.

12. A method for controlling weeds in a cotton crop comprising the transgenic cotton plant of claim 7 , the method comprising:
applying an herbicide to the cotton crop, wherein the herbicide is a phenoxyacetic acid herbicide, a pyridoxyacetic acid herbicide, or glufosinate.

13. The method according to claim 12 , wherein the herbicide is a phenoxyacetic acid herbicide.

14. The method according to claim 13 , wherein the phenoxyacetic acid herbicide is 2,4-D or MCPA.

15. The method according to claim 12 , wherein the herbicide is a pyridoxyacetic acid herbicide.

16. The method according to claim 15 , wherein the pyridoxyacetic acid herbicide is triclopyr or fluroxypyr.

17. The method according to claim 12 , wherein the herbicide is glufosinate.

Assignees

• Dow Agrosciences Llc

Inventors

• Cui Yunxing C
• King Raina
• Kaiser Tina M
• Robinson Andrew E
• Pareddy Dayakar
• Toledo Sandra G
• Braxton Leon B
• Anderson David M
• Wright Terry R

Classifications

• C12N15/8277

  Phosphinotricin · CPC title

• C12N15/8274 Primary

  for herbicide resistance · CPC title

• C12Q2600/16

  Primer sets for multiplex assays · CPC title

• C12Q2600/13

  Plant traits · CPC title

• C12N9/0071

  acting on paired donors with incorporation of molecular oxygen (1.14) · CPC title