Plant regulatory elements derived from medicago truncatula 3′UTR sequences, and uses thereof

Patent metadata

Field	Value
Publication number	US-10501749-B2
Application number	US-201815882964-A
Country	US
Kind code	B2
Filing date	Jan 29, 2018
Priority date	Mar 14, 2013
Publication date	Dec 10, 2019
Grant date	Dec 10, 2019

Abstract

Recombinant DNA molecules and constructs useful for modulating gene expression in plants, including molecules derived from Medicago truncatula sequences, are provided. plants, plant cells, plant parts, and seeds comprising recombinant DNA molecules operably linked to heterologous transcribable DNA molecules are further provided, as are methods of their use.

First claim

What is claimed is:

1. A recombinant DNA molecule comprising a DNA sequence selected from the group consisting of:
a) a DNA sequence comprising SEQ ID NO:3; and
b) a fragment comprising at least 200 contiguous nucleotides of SEQ ID NO:3, wherein the fragment has gene-regulatory activity;
wherein said DNA sequence is operably linked to a heterologous transcribable DNA molecule.

2. The recombinant DNA molecule of claim 1 , wherein said DNA sequence comprises SEQ ID NO:3.

3. The recombinant DNA molecule of claim 1 , wherein the heterologous transcribable polynucleotide molecule comprises a gene of agronomic interest.

4. The recombinant DNA molecule of claim 3 , wherein the gene of agronomic interest confers herbicide tolerance in plants.

5. The recombinant DNA molecule of claim 3 , wherein the gene of agronomic interest confers pest resistance in plants.

6. A transgenic plant cell comprising a recombinant DNA molecule comprising a DNA sequence selected from the group consisting of:
a) a DNA sequence comprising SEQ ID NO:3; and
b) a fragment comprising at least 200 contiguous nucleotides of SEQ ID NO:3, wherein the fragment has gene-regulatory activity;
wherein said DNA sequence is operably linked to a heterologous transcribable DNA molecule.

7. The transgenic plant cell of claim 6 , wherein said transgenic plant cell is a monocotyledonous plant cell.

8. The transgenic plant cell of claim 6 , wherein said transgenic plant cell is a dicotyledonous plant cell.

9. A transgenic plant, or part thereof, comprising a recombinant DNA molecule comprising a DNA sequence selected from the group consisting of:
a) a DNA sequence comprising SEQ ID NO:3; and
b) a fragment comprising at least 200 contiguous nucleotides of SEQ ID NO:3, wherein the fragment has gene-regulatory activity;
wherein said DNA sequence is operably linked to a heterologous transcribable DNA molecule.

10. A progeny plant of the transgenic plant of claim 9 , or a part thereof, wherein the progeny plant or part thereof comprises said recombinant DNA molecule.

11. A transgenic seed of the transgenic plant of claim 9 wherein the transgenic seed comprises the recombinant DNA molecule.

12. A method of producing a commodity product comprising obtaining a transgenic plant or part thereof according to claim 9 and producing the commodity product therefrom.

13. The method of claim 12 , wherein the commodity product is protein concentrate, protein isolate, grain, starch, seeds, meal, flour, biomass, or seed oil.

14. A method of producing a transgenic plant comprising:
a) transforming a plant cell with the recombinant DNA molecule of claim 1 to produce a transformed plant cell; and
b) regenerating a transgenic plant from the transformed plant cell.

Assignees

• Monsanto Technology Llc

Inventors

• Chittoor Jaishree M
• Miyamoto Amy J
• Nichols Amy M
• Oufattole Mohammed
• Petersen Michael W

Classifications

• C12N15/8241

  Phenotypically and genetically modified plants via recombinant DNA technology · CPC title

• C12N15/8279 Primary

  for biotic stress resistance, pathogen resistance, disease resistance · CPC title

• C12N15/8216 Primary

  Methods for controlling, regulating or enhancing expression of transgenes in plant cells · CPC title

• C12N15/8274

  for herbicide resistance · CPC title

• C12N15/8261

  with agronomic (input) traits, e.g. crop yield · CPC title