Promoter and use thereof

Patent metadata

Field	Value
Publication number	US-10501745-B2
Application number	US-201615563750-A
Country	US
Kind code	B2
Filing date	Mar 11, 2016
Priority date	Apr 2, 2015
Publication date	Dec 10, 2019
Grant date	Dec 10, 2019

Abstract

The present disclosure relates to a novel promoter, a vector comprising the promoter, a microorganism comprising the vector, and a method for producing a target protein using the promoter.

First claim

The invention claimed is:

1. An isolated nucleic acid molecule having a promoter activity, wherein the isolated nucleic acid molecule comprises the nucleotide sequence of SEQ ID NO: 1.

2. A target protein expression cassette comprising the isolated nucleic acid molecule having a promoter activity of claim 1 and a gene encoding a target protein.

3. A recombinant vector comprising the isolated nucleic acid molecule of claim 1 or the target protein expression cassette of claim 2 .

4. The recombinant vector of claim 3 , wherein the target protein is a psicose-3-epimeras.

5. A method for producing psicose, comprising:
(a) culturing the microorganism introduced with the vector of claim 4 ; and
(b) reacting the cultured microorganism with fructose to produce psicose.

6. A recombinant microorganism introduced with the vector of claim 4 .

7. The recombinant microorganism of claim 6 , wherein the microorganism is a microorganism of the genus Corynebacterium.

8. The recombinant microorganism of claim 7 , wherein the microorganism of the genus Corynebacterium is Corynebacterium glutamicum or Corynebacterium ammoniagenes.

9. The recombinant microorganism of claim 6 , wherein the microorganism is a microorganism of the genus Escherichia.

10. The recombinant microorganism of claim 9 , wherein the microorganism of the genus Escherichia is Escherichia coli.

11. A method for producing a target protein, comprising:
(a) culturing the recombinant microorganism of claim 6 in a medium to produce a target protein; and
(b) recovering the produced target protein from the cultured microorganism or the medium.

12. A recombinant microorganism introduced with the vector of claim 3 .

13. The recombinant microorganism of claim 12 , wherein the microorganism is a microorganism of the genus Corynebacterium.

14. The recombinant microorganism of claim 13 , wherein the microorganism of the genus Corynebacterium is Corynebacterium glutamicum or Corynebacterium ammoniagenes.

15. The recombinant microorganism of claim 12 , wherein the microorganism is a microorganism of the genus Escherichia.

16. The recombinant microorganism of claim 15 , wherein the microorganism of the genus Escherichia is Escherichia coli.

17. A method for producing a target protein, comprising:
(a) culturing the recombinant microorganism of claim 12 in a medium to produce the target protein; and
(b) recovering the produced target protein from the cultured microorganism or the medium.

Assignees

• Cj Cheiljedang Corp

Inventors

• Lee Young Mi
• Lee Seung Bin
• Kim Seong Bo
• Lee Ji Hyun
• Chang Jin Sook
• Cho Seung Hyun
• Park Seung Won

Classifications

• C12P21/02 Primary

  having a known sequence of two or more amino acids, e.g. glutathione · CPC title

• C12N15/70 Primary

  Vectors or expression systems specially adapted for E. coli · CPC title

• C12P21/00

  Preparation of peptides or proteins (single cell protein C12N1/00) · CPC title

• C12P19/02

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title

• C12N15/113 Primary

  Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)} · CPC title