Device with controlled fluid dynamics, for isolation of an analyte from a sample

Patent metadata

Field	Value
Publication number	US-10501735-B2
Application number	US-201313972788-A
Country	US
Kind code	B2
Filing date	Aug 21, 2013
Priority date	Aug 23, 2012
Publication date	Dec 10, 2019
Grant date	Dec 10, 2019

Abstract

Devices for use in extracting an analyte of interest from a sample are described. In one embodiment, a device is comprised of a first plurality of chambers, where one or more chambers in the plurality of chambers has a deep end and a shallow end with a depth d 1 . A channel disposed between at least two adjacent chambers in the plurality of chambers has a depth greater than d 1 . The dimensions of the chamber and channel provide control of fluid movement in the device, particularly when introducing fluid into the device for its use and during use of the device.

First claim

It is claimed:

1. A device, comprising
a first plurality of chambers disposed along a longitudinal axis of the device, one or more chambers in the first plurality of chambers having both a deep end and a shallow end arranged orthogonally to the longitudinal axis, the shallow end with a depth d 1 , wherein the deep end is adjacent to a side of the chambers; and
a channel disposed between and in fluid communication with at least two adjacent chambers in the first plurality of chambers, the channel having a depth greater than d 1 .

2. The device of claim 1 , wherein the chambers in the first plurality of chambers are serially positioned and separated by a channel.

3. The device of claim 2 , wherein the first plurality of chambers is comprised of at least three chambers, wherein two chambers in the first plurality have a deep end and a shallow end arranged orthogonally to the longitudinal axis, the shallow end with a depth d 1 , and wherein the channel is disposed between the two chambers having the shallow end with a depth d 1 .

4. The device of claim 2 , wherein the first plurality of chambers is comprised of at least five chambers, wherein three chambers in the first plurality have a deep end and a shallow end arranged orthogonally to the longitudinal axis, the shallow end with a depth d 1 , and wherein the channel is disposed between two chambers having the shallow end with a depth d 1 .

5. The device of claim 1 , wherein each chamber in the first plurality of chambers has a volume, and wherein the volume of one chamber in the plurality of chambers differs from the volume of another chamber in the first plurality.

6. The device of claim 1 , wherein the one or more chambers in the first plurality of chambers has a floor and a side wall that meet to define four corners, wherein at least the corners in the deep end of the chamber are rounded.

7. The device of claim 1 , wherein the one or more chambers in the first plurality of chambers has a floor and a side wall that meet to define four corners, wherein at least the corners in the shallow end of the chamber are angled.

8. The device of claim 1 , further comprising a cover, the cover having an access port in fluid communication with a chamber in the first plurality of chambers.

9. The device of claim 8 , wherein the access port is in fluid communication with the one or more chambers in the first plurality of chambers and wherein the access port is positioned for introduction of fluid directly into the deep end of the chamber.

10. The device of claim 1 , further comprising:
a second plurality of chambers, one or more chambers in the second plurality of chambers having a deep end and a shallow end arranged orthogonally to the longitudinal axis, the shallow end with a depth d 1 ; and
a channel disposed between at least two adjacent chambers in the second plurality of chambers, the channel having a depth greater than d 1 .

11. The device of claim 10 , wherein the first and second plurality of chambers are linearly parallel.

Assignees

• Univ Northwestern
• Quidel Corp

Inventors

• Kelso David M
• Sur Kunal
• Westberg Tom
• Parpia Zaheer
• Fisher Mark J

Classifications

• C12N15/1006 Primary

  by means of a solid support carrier, e.g. particles, polymers · CPC title

• B01L3/502761

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title

• Y10T436/25375

  Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] · CPC title

• B01L3/502753

  characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation · CPC title

• B01L2300/0851

  Bottom walls · CPC title