Low-viscosity starch hydrolysate with improved retrogradation behaviour

Patent metadata

Field	Value
Publication number	US-10501560-B2
Application number	US-201615781251-A
Country	US
Kind code	B2
Filing date	Dec 12, 2016
Priority date	Dec 10, 2015
Publication date	Dec 10, 2019
Grant date	Dec 10, 2019

Abstract

The invention relates to a starch hydrolysate having a Dextrose Equivalent DE of between 5 and 30 and a specific carbohydrate profile. In particular, the hydrolysate of the invention has improved retrogradation behaviour. The invention also relates to a method for the production of said starch hydrolysate.

First claim

The invention claimed is:

1. A starch hydrolysate having a Dextrose Equivalent DE ranging from 5 to 30 and in which the DE, the dry weight content of saccharides having a degree of polymerization ranging from 10 to 20 (DP 10-20) and the dry weight content of saccharides having a degree of polymerization of 50 or more (DP 50+) are such that they meet the following inequations:
−0.83×DE+25≤% DP50+≤−1.07×DE+40;

−0.83×DE+27.5≤% DP10-20≤−1.25×DE+55.

2. The starch hydrolysate as claimed in claim 1 , having:
a DE ranging from 24 to 30;
a dry weight content of DP 10-20 ranging from 10% to 18%;
a dry weight content of DP 50+ ranging from 3% to 13%.

3. The starch hydrolysate as claimed in claim 1 , having:
a DE ranging from 20 to 24;
a dry weight content of DP 10-20 ranging from 10% to 20%;
a dry weight content of DP 50+ranging from 6% to 18%.

4. The starch hydrolysate as claimed in claim 1 , having:
a DE ranging from 17 to 20;
a dry weight content of DP 10-20 ranging from 12% to 25%;
a dry weight content of DP 50+ ranging from 10% to 19%.

5. The starch hydrolysate as claimed in claim 1 , having:
a DE ranging from 15 to 17;
a dry weight content of DP 10-20 ranging from 16% to 27%;
a dry weight content of DP 50+ ranging from 13% to 22%.

6. The starch hydrolysate as claimed in claim 1 , having:
a DE ranging from 13 to 15;
a dry weight content of DP 10-20 ranging from 17% to 30%;
a dry weight content of DP 50+ ranging from 17% to 25%.

7. The starch hydrolysate as claimed in claim 1 , having:
a DE ranging from 10 to 13;
a dry weight content of DP 10-20 ranging from 20% to 32%;
a dry weight content of DP 50+ ranging from 18% to 28%.

8. The hydrolysate as claimed in claim 1 , characterized in that it is in the form of an aqueous solution.

9. The hydrolysate as claimed in claim 1 , characterized in that it is in the form of a powder.

10. A process for producing the starch hydrolysate according to claim 1 , comprising:
a) a step of bringing an aqueous solution of starch or of starchy material into contact with at least one alpha-amylase comprising an Enzyme V;
b) followed by a step of hydrolysis of said aqueous solution;
c) followed by a step of inhibition of the Enzyme V so as to form a solution of intermediate hydrolysate having a DE equal to DE c ;
d) followed by a step of bringing the solution of intermediate hydrolysate obtained in step c) into contact with at least one additional alpha-amylase, different than the Enzyme V;
e) followed by a second hydrolysis step so as to form a hydrolysate for a period of time sufficient for it to have a DE e ranging from 5 to 30;
f) followed by a step of recovering the hydrolysate formed in step e); characterized in that the Enzyme V is an enzyme chosen from:
polypeptides encoded by a nucleotide sequence as represented in SEQ ID NO1 or a nucleotide sequence having a percentage identity relative to the sequence SEQ ID NO1 at least equal to 80%, or
polypeptides comprising the enzymatically active fragment of the nucleotide sequence encoded by the sequence SEQ ID NO1.

11. The process as claimed in claim 10 , characterized in that the aqueous solution of starch formed in step a) has a pH ranging from 4.3 to 5.9.

12. The process as claimed in claim 10 , characterized in that the solution of hydrolysate formed in step d) has a pH ranging from 4.3 to 5.9.

13. The process as claimed in claim 10 , characterized in that the inhibition step is carried out by heating the solution of intermediate hydrolysate at a temperature greater than or equal to 150° C.

14. The process as claimed in claim 10 , characterized in that the DE e is greater than or equal to DE c +3.

15. The process as claimed in claim 10 , characterized in that it also comprises at least one step g) of converting the hydrolysate recovered in step f).

16. The process as claimed in claim 15 , characterized in that step g) is a step of forming the hydrolysate in powder form by means of a spray-drying step.

17. A food product, comprising the starch hydrolysate as claimed in claim 1 and at least one food ingredient.

18. A pharmaceutical product, comprising the starch hydrolysate as claimed in claim 1 and at least one pharmaceutical ingredient.

19. A mixture, comprising the starch hydrolysate as claimed in claim land a second hydrolysate.

20. The process according to claim 10 , wherein the percentage identity is at least 85%.

21. The process according to claim 10 , wherein the percentage identity is at least 90%.

22. The process according to claim 10 , wherein the percentage identity is at least 98%.

23. The process according to claim 10 , wherein the percentage identity is at least 99%.

Assignees

• Roquette Freres

Inventors

• Ibert Mathias
• Lecocq Aline
• Lanos Pierre

Classifications

• A23L29/35

  Degradation products of starch, e.g. hydrolysates, dextrins; Enzymatically modified starches · CPC title

• A61K47/26

  Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin · CPC title

• C08L3/02

  Starch; Degradation products thereof, e.g. dextrin · CPC title

• C12P19/14

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title

• A61K47/36

  Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin · CPC title