Apparatus and methods for optical coherence tomography and two-photon luminescence imaging

Patent metadata

Field	Value
Publication number	US-10495440-B2
Application number	US-201615264505-A
Country	US
Kind code	B2
Filing date	Sep 13, 2016
Priority date	Mar 14, 2013
Publication date	Dec 3, 2019
Grant date	Dec 3, 2019

Abstract

Exemplary embodiments of the present disclosure include a combined catheter-based optical coherence tomography-two-photon luminescence (OCT-TPL) imaging system. Exemplary embodiments further include methods to detect, and further characterize the distribution of cellular components (e.g., macrophage, collagen/elastin fiber, lipid droplet) in thin-cap fibroatheromas with high spatial resolution in vivo.

First claim

The invention claimed is:

1. A method of imaging a sample site, the method comprising:
emitting a first wavelength from an optical coherence tomography light source toward a sample site;
emitting a second wavelength from a short-pulsed light source toward the sample site, wherein the first wavelength from the optical coherence tomography light source and the second wavelength from the short-pulsed light source are simultaneously transmitted through a double-clad photonic crystal fiber, wherein:
the double-clad photonic crystal fiber comprises a single-mode core embedded in a multimode fiber structure;
the single-mode core has a lower numerical aperture (NA) than the multimode fiber structure;

detecting an optical coherence tomography signal from the sample site, wherein the optical coherence tomography signal is generated from the first wavelength; and
detecting a two-photon luminescence emission signal from the sample site, wherein:
the two-photon luminescence emission signal is induced by the second wavelength;
the two-photon luminescence emission signal is transmitted at the same time as the first wavelength and the second wavelength;
the two-photon luminescence emission signal is transmitted in an opposite direction from the first wavelength and the second wavelength;
the optical coherence tomography signal comprises radial and azimuthal dimensional data; and

the two-photon luminescence signal comprises an azimuthal signal; and

adding a radial dimension to the two-photon luminescence data, wherein:
adding the radial dimension to the two-photon luminescence data comprises using a radial probability distribution function that is normalized by the two-photon luminescence azimuthal signal; and
the radial probability distribution function is determined using:
optical properties of the imaging system;
the distance between the catheter-based imaging system and a lumenal wall into which the catheter-based imaging system is inserted; and
the optical properties of tissue of the lumenal wall; and

generating three-dimensional images based on data obtained from the catheter-based imaging system as the catheter-based imaging system is moved axially along a lumen.

2. The method of claim 1 wherein the optical coherence tomography signal and the two-photon luminescence signal are detected from a plurality sample sites.

3. The method of claim 1 wherein the sample comprises a tissue.

4. The method of claim 3 wherein the tissue is epithelial tissue.

5. The method of claim 3 where the tissue is arterial tissue.

6. The method of claim 5 where the arterial tissue is located in a coronary artery.

7. The method of claim 3 wherein the tissue is a vascular luminal surface.

8. The method of claim 3 wherein the tissue is oral mucosa.

9. The method of claim 1 wherein the optical coherence tomography signal is used to generate an optical coherence tomography tomogram.

10. The method of claim 1 wherein the two-photon luminescence signal is co-registered with an optical coherence tomography tomogram.

11. The method of claim 1 further comprising displaying two-dimensional two-photon luminescence data on a three-dimensional optical coherence tomography tomogram.

12. The method of claim 1 wherein a first processing element uses the optical coherence tomography signal and constructs an optical coherence tomography tomogram.

13. The method of claim 12 wherein the first processing element is a central processing unit or a graphics processing unit.

14. The method of claim 12 wherein a second processing element renders for viewing a co-registered two-photon luminescence image on an optical coherence tomography tomogram.

15. The method of claim 1 wherein the sample site comprises a nanoparticle.

16. The method of claim 15 wherein the two-photon luminescence signal is emitted from the nanoparticle.

17. The method of claim 1 wherein the two-photon luminescence emission signal is emitted from tissue of the sample site.

18. A method for displaying imaging data, the method comprising:
obtaining optical coherence tomography data with an imaging system;
obtaining two-photon luminescence data from a plurality of luminescing particles with the imaging system, wherein:
the imaging system is a catheter-based imaging system;
the optical coherence tomography data is generated from light emitted at a first wavelength from an optical coherence tomography light source;
the optical coherence tomography data comprises radial and azimuthal dimensional data;
the two-photon luminescence data comprises an azimuthal signal;
the two-photon luminescence data is generated from light emitted at a second wavelength from a short-pulsed light source; and
the first wavelength from the optical coherence tomography light source and the second wavelength from the short-pulsed light source are simultaneously transmitted through a double-clad photonic crystal fiber, wherein:
the double-clad photonic crystal fiber comprises a single-mode core embedded in a multimode fiber structure;
the single-mode core has a lower numerical aperture (NA) than the multimode fiber structure;
the two-photon luminescence emission data is transmitted at the same time as the first wavelength and the second wavelength; and
the two-photon luminescence emission data is transmitted in an opposite direction from the first wavelength and the second wavelength; and

adding a radial dimension to the two-photon luminescence data, wherein:
adding the radial dimension to the two-photon luminescence data comprises using a radial probability distribution function that is normalized by the two-photon luminescence azimuthal signal; and
the radial probability distribution function is determined using:
optical properties of the imaging system:
the distance between the catheter-based imaging system and a lumenal wall into which the catheter-based imaging system is inserted; and
the optical properties of tissue of the lumenal wall;

simultaneously displaying the optical coherence tomography data and the two-photon luminescence data in a combined image; and
generating three-dimensional images based on data obtained from the catheter-based imaging system as the catheter-based imaging system is moved axially along a lumen.

19. The method of claim 18 wherein the luminescing particle is a nanoparticle.

20. The method of claim 18 further comprising generating three-dimensional images based on data obtained from the catheter-based imaging system as the catheter-based imaging system is moved axially along a lumen.

21. The method of claim 18 , wherein the radial probability distribution function is determined using assuming a uniform distribution of nanoparticles.

Assignees

• Res Found Dev

Inventors

• Feldman Marc
• Milner Thomas
• Wang Tianyi
• Whedbee Jennifer

Classifications

• A61K49/0013

  Luminescence · CPC title

• G01B9/0203 Primary

  With imaging systems · CPC title

• G01B9/02007

  Two or more frequencies or sources used for interferometric measurement (using only beat G01B9/02003) · CPC title

• G02B23/2484

  Arrangements in relation to a camera or imaging device (processing or control of video signals generated by an endoscope H04N23/60, H04N23/70) · CPC title

• A61B5/0035

  adapted for acquisition of images from more than one imaging mode, e.g. combining MRI and optical tomography · CPC title