Hybrid polyketide synthases

Patent metadata

Field	Value
Publication number	US-10494625-B2
Application number	US-201615144727-A
Country	US
Kind code	B2
Filing date	May 2, 2016
Priority date	Oct 29, 2010
Publication date	Dec 3, 2019
Grant date	Dec 3, 2019

Abstract

The present invention provides for a polyketide synthase (PKS) capable of synthesizing an even-chain or odd-chain diacid or lactam or diamine. The present invention also provides for a host cell comprising the PKS and when cultured produces the even-chain diacid, odd-chain diacid, or KAPA. The present invention also provides for a host cell comprising the PKS capable of synthesizing a pimelic acid or KAPA, and when cultured produces biotin.

First claim

What is claimed is:

1. A non-naturally occurring polyketide synthase (PKS) that binds malonyl-CoA and releases malonic acid, wherein said non-naturally occurring PKS is a hybrid PKS comprising domains from two PKSs, and comprises a single, three-domain chimeric PKS module comprising a didomain containing a malonyl-specific acyltransferase (AT) domain linked to a cognate acyl carrier protein (ACP) domain, and a type I thioesterase (TE) domain linked to said didomain;
wherein said didomain is from a load module from a PKS selected from the group consisting of a Streptomyces bikiniensis chalcomucin PKS, a Streptomyces caelestis niddamycin PKS, a Streptomyces avermitilis oligomycin PKS, and a Sorangium cellulosum epothilone PKS; and
wherein said TE domain is from a Streptomyces venezuelae pikromycin PKS or a Saccharopolyspora erythraea erythromycin PKS.

Assignees

• Univ California

Inventors

• Fortman Jeffrey L
• Hagen Andrew
• Katz Leonard
• Keasling Jay D
• Poust Sean
• Zhang Jingwei
• Zotchev Sergey

Classifications

• C12P7/46

  Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid · CPC title

• C12P13/001

  Amines; Imines · CPC title

• C12P7/44

  Polycarboxylic acids · CPC title

• C12P13/04

  Alpha- or beta- amino acids {(other amino acids C12P13/005)} · CPC title

• C12P17/10

  Nitrogen as only ring hetero atom · CPC title