Bacteria and methods of use thereof

Patent metadata

Field	Value
Publication number	US-10494600-B2
Application number	US-201514874665-A
Country	US
Kind code	B2
Filing date	Oct 5, 2015
Priority date	Jul 28, 2010
Publication date	Dec 3, 2019
Grant date	Dec 3, 2019

Abstract

The invention provides a novel bacterium with improved properties, including improved ethanol production, ethanol productivity, CO uptake, specific growth rate, ethanol to acetate ratio, and alcohol tolerance. The bacterium may be derived from Clostridium autoethanogenum and/or may comprise at least one DNA or amino acid sequence selected from SEQ ID NOs: 1, 3, 6, 8, 10, 12, 14, and 16. In one embodiment, the bacterium is Clostridium autoethanogenum deposited under DSMZ accession number DSM23693 or a bacterium derived therefrom.

First claim

The invention claimed is:

1. A bacterium derived from Clostridium autoethanogenum wherein the bacterium comprises at least one DNA or amino acid sequence selected from SEQ ID NOs: 1, 3, 6, 8, 10, 12, 14, and 16.

2. The bacterium of claim 1 , wherein the bacterium is a bacterium deposited under DSMZ accession number DSM23693 or a bacterium derived therefrom.

3. The bacterium of claim 1 , wherein the bacterium is derived from Clostridium autoethanogenum deposited under DSMZ accession number DSM19630 or naturally occurring Clostridium autoethanogenum.

4. The bacterium of claim 1 , wherein the bacterium ferments a gaseous substrate to produce one or more products.

5. The bacterium of claim 4 , wherein the gaseous substrate comprises one or more of CO, CO 2 , and H 2 .

6. The bacterium of claim 4 , wherein the products comprise one or more of alcohol and acetate.

7. The bacterium of claim 6 , wherein the alcohol is ethanol.

8. A method of producing a product comprising culturing the bacterium of claim 1 in the presence of a gaseous substrate whereby the bacterium produces one or more products.

9. The method of claim 8 , wherein the bacterium is a bacterium deposited under DSMZ accession number DSM23693 or a bacterium derived therefrom.

10. The method of claim 8 , wherein the bacterium is derived from Clostridium autoethanogenum deposited under DSMZ accession number DSM19630 or naturally occurring Clostridium autoethanogenum.

11. The method of claim 8 , wherein the gaseous substrate comprises one or more of CO, CO 2 , and H 2 .

12. The method of claim 8 , wherein the products comprise one or more of alcohol and acetate.

13. The method of claim 12 , wherein the alcohol is ethanol.

Assignees

• Lanzatech New Zealand Ltd

Inventors

• Heijstra Bjorn Daniel
• Kern Evgenia
• Koepke Michael
• Segovia Simon
• Liew Fungmin

Classifications

• C12P7/065

  with microorganisms other than yeasts · CPC title

• C12N1/20 Primary

  Bacteria; Culture media therefor · CPC title

• C12P7/24

  containing a carbonyl group · CPC title

• C12P7/54

  Acetic acid (vinegar C12J) · CPC title

• C12R1/145

  Chemistry & Metallurgy · mapped topic