Cosmetic use of heparan sulphate

Patent metadata

Field	Value
Publication number	US-10493012-B2
Application number	US-201515527995-A
Country	US
Kind code	B2
Filing date	Nov 19, 2015
Priority date	Nov 19, 2014
Publication date	Dec 3, 2019
Grant date	Dec 3, 2019

Abstract

Affinity purification of fibroblast growth factor 2-binding heparan sulphate from porcine mucosa (HS8) is disclosed. Also disclosed is a process for the care of keratinous material(s), comprising the application or administration of HS8, or a composition comprising thereof, to keratinous material(s). The composition may be used to improve the condition of skin, such as smoothing the skin, restoring skin elasticity and firmness, or decreasing or preventing wrinkles, and may be formulated for topical or transdermal administration.

First claim

The invention claimed is:

1. A cosmetic process for caring for keratinous material(s), the process comprising application or administration of heparan sulphate HS8, or a composition comprising heparan sulphate HS8, to keratinous material(s), wherein the heparan sulphate HS8 is capable of binding a peptide or polypeptide having, or consisting of, the amino acid sequence YCKNGGF (SEQ ID NO:2) or GHFKDPKRLYCKNGGF (SEQ ID NO:1).

2. The process of claim 1 , wherein the keratinous material(s) is selected from one or more of human skin, hair or nails.

3. The process of claim 1 , wherein heparan sulphate HS8 is used or is present in the composition in a content ranging from 0.0001% to 5% by weight, relative to the total weight of the composition.

4. The process of claim 1 , wherein said process is intended to decrease the signs of ageing of the keratinous material(s).

5. The process of claim 1 , wherein said process is intended to maintain and/or stimulate moisturization and/or combat drying-out of the keratinous material(s).

6. The process of claim 2 , wherein said process is intended to:
(i) decrease characteristics of wrinkles, small wrinkles and/or fine lines of the skin;
(ii) improve and/or decrease the microrelief of the skin;
(iii) smooth the skin;
(iv) improve the density of the skin;
(v) maintain and /or restore skin elasticity;
(vi) maintain and/or restore skin firmness; or
(vii) maintain and/or restore the cohesion of skin compartments.

7. A cosmetic method of regulating a skin condition, comprising applying or administering to the skin of a human individual a safe and effective amount of heparan sulphate HS8, or a composition comprising heparan sulphate HS8, wherein the heparan sulphate HS8 is capable of binding a peptide or polypeptide having, or consisting of, the amino acid sequence YCKNGGF (SEQ ID NO:2) or GHFKDPKRLYCKNGGF (SEQ ID NO:1).

8. The cosmetic method of claim 7 wherein the method comprises improving skin appearance and/or feel.

9. The cosmetic method of claim 7 , wherein the method comprises regulating visible and/or tactile discontinuities in skin texture.

10. The process of claim 1 wherein the heparan sulphate HS8 is provided in isolated or substantially purified form.

11. The process of claim 1 wherein following digestion with heparin lyases I, II and Ill and then subjecting the resulting disaccharide fragments to capillary electrophoresis analysis the heparan sulphate HS8 has a disaccharide composition comprising:

Disaccharide
Normalised weight percentage

ΔUA,2S-GlcNS,6S
12.7 ± 3.0

ΔUA,2S-GlcNS
 7.2 ± 2.0

ΔUA-GlcNS,6S
15.5 ± 3.0

ΔUA,2S-GlcNAc,6S
 6.5 ± 2.0

ΔUA-GlcNS
15.7 ± 3.0

ΔUA,2S-GlcNAc
 1.0 ± 0.5

ΔUA-GlcNAc,6S
 8.9 ± 3.0

ΔUA-GlcNAc
32.5 ± 3.0.

Assignees

• Agency Science Tech & Res
• Oreal

Inventors

• Cool Simon
• Nurcombe Victor
• Bouez Charbel
• Bramono Diah Saraswati

Classifications

• A61K8/73 Primary

  Polysaccharides · CPC title

• A61Q19/08

  Anti-ageing preparations · CPC title

• A61K2800/10

  General cosmetic use · CPC title

• A61Q19/007

  Preparations for dry skin · CPC title

• C08B37/0075

  Heparin; Heparan sulfate; Derivatives thereof, e.g. heparosan; Purification or extraction methods thereof · CPC title