Method of pretreatment for enhanced enzymatic hydrolysis

Patent metadata

Field	Value
Publication number	US-10487347-B2
Application number	US-201615373788-A
Country	US
Kind code	B2
Filing date	Dec 9, 2016
Priority date	Dec 9, 2015
Publication date	Nov 26, 2019
Grant date	Nov 26, 2019

Abstract

The present invention discloses a method for producing readily available and hydrolysable polysaccharide-enriched biomass from lignocellulosic biomass while quantitatively retaining carbohydrate and producing fewer amounts of inhibitors. The method includes treating lignocellulosic biomass with an aqueous solution at elevated temperatures in a sequential step using hydrazine hydrate and sodium sulphite. In accordance with the present invention, the biomass may be further treated with a saccharification enzyme consortium to produce fermentable sugars.

First claim

The invention claimed is:

1. A method for producing hydrolyzable polysaccharides-enriched biomass comprising of: (a) treating biomass with aqueous mixture of hydrazine hydrate and sulphur compound sodium sulfite to obtain a hydrolyzate and end products; and
(b) removing hydrolyzate from end product as obtained in step (a) to obtain residues of hydrolyzable polysaccharides-enriched biomass, wherein the method further comprising of enzymatic saccharification of the hydrolyzable polysaccharides-enriched biomass in step (b) by contacting it with a saccharification enzyme consortium to produce fermentable sugars.

2. The method as claimed in claim 1 , further comprising of treating the residues of hydrolyzable polysaccharides-enriched biomass with water or other organic solvent to remove lignin.

3. The method as claimed in claim 1 , wherein the biomass is suspended in the aqueous mixture of hydrazine hydrate and sodium sulfite.

4. The method as claimed in claim 1 , wherein the biomass is treated with aqueous mixture of hydrazine hydrate and sodium sulfite at between 80-200° C. temperature.

5. The method as claimed in claim 4 wherein the treatment of biomass with aqueous mixture of hydrazine hydrate and sodium sulfite is done at temperature ranging between 80-200° C. for about 5 minutes to 4 hours.

6. The method as claimed in claim 3 , wherein in the aqueous mixture of hydrazine hydrate and sodium sulfite, the hydrazine hydrate is present in range of 1-99% (w/w) and the sodium sulfite is present in range of 0.1-10%) (w/w).

7. The method as claimed in claim 1 , wherein the biomass is a lignocellulosic biomass.

8. The method as claimed in claim 1 , wherein the biomass is shredded to reduce particle size in the range of 1 mm to 20 mm.

9. The method as claimed in claim 1 , wherein the biomass is selected from cotton stalk, mustard stalk, wheat straw and rice straw.

10. The method as claimed in claim 2 , wherein the biomass is suspended in the aqueous mixture of hydrazine hydrate and sodium sulfite.

11. The method as claimed in claim 10 , wherein in the aqueous mixture of hydrazine hydrate and sodium sulfite, the hydrazine hydrate is present in the range of 1-99% (w/w) and the sodium sulfite is present in the range of 0.1-10% (w/w).

12. The method as claimed in claim 4 , wherein in the aqueous mixture of hydrazine hydrate and sodium sulfite, the hydrazine hydrate is present in the range of 1-99% (w/w) and the sodium sulfite is present in the range of 0.1-10% (w/w).

13. The method as claimed in claim 2 , wherein the biomass is a liguocellulosic biomass.

Assignees

• Indian Oil Corp Ltd
• Dept Of Biotechnology India

Inventors

• Kumar Ravindra
• GAUR Ruchi
• Raj Tirath
• Kakkar Manali Kapoor
• Satlewal Alok
• Gupta Ravi Prakash
• Tuli Deepak Kumar

Classifications

• C12P19/02

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title

• C12P2201/00

  Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis · CPC title

• C12P19/14 Primary

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title

• Y02E50/16

  Cross-Sectional Technologies · mapped topic

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title