Method of producing fatty acids or lipids by using acyltransferase

Patent metadata

Field	Value
Publication number	US-10487344-B2
Application number	US-201615755247-A
Country	US
Kind code	B2
Filing date	Sep 1, 2016
Priority date	Sep 11, 2015
Publication date	Nov 26, 2019
Grant date	Nov 26, 2019

Abstract

A method of producing lipids, containing the steps of:
culturing a transformant in which the expression of a gene encoding the following protein (A) or (B) is enhanced, and producing fatty acids or lipids containing these fatty acids as components: (A) a protein consisting of the amino acid sequence set forth in SEQ ID NO: 1; and (B) a protein consisting of an amino acid sequence having 44% or more identity with the amino acid sequence of the protein (A), and having acyltransferase activity.

First claim

What is claimed is:

1. A method of producing fatty acids or lipids containing the fatty acids, comprising the steps of:
culturing a transformant under conditions suitable for production of fatty acids or lipids containing the fatty acids as components,
producing fatty acids or lipids containing the fatty acids as components as a result of the culturing; and
collecting the fatty acids or lipids containing the fatty acids,
wherein the transformant is a host organism that has been transformed with a gene encoding a protein selected from the following protein (A) or (B):
(A) a protein consisting of the amino acid sequence set forth in SEQ ID NO: 1; and
(B) a protein consisting of an amino acid sequence having 90% or more identity with the amino acid sequence of the protein (A), and having acyltransferase activity;
wherein the gene is expressed during the culturing;
and
wherein productivity of the fatty acids or lipids containing the fatty acids as components is increased in the transformant as a result of the culturing, as compared to productivity of the host organism that has not been transformed with the gene.

2. The method of claim 1 , wherein
productivity of medium-chain fatty acids or lipids containing the medium-chain fatty acids as components is increased in the transformant as a result of the culturing, as compared to productivity of the host organism that has not been transformed with the gene.

3. The method of claim 1 , wherein
the total amount of all fatty acids produced by the transformant is increased as a result of the culturing, as compared to total amount of all fatty acids produced by the host organism that has not been transformed with the gene.

4. The method according to claim 1 , wherein expression of a gene encoding an acyl-ACP thioesterase having substrate specificity to a medium-chain acyl-ACP is enhanced in the transformant, as compared to that of a transformant in which expression of the gene encoding the acyl-ACP thioesterase is not enhanced.

5. The method according to claim 1 , wherein the transformant is a microalga.

6. The method according to claim 5 , wherein the microalga is an alga belonging to the genus Nannochloropsis.

7. The method according to claim 1 , wherein the lipids contain a fatty acid having 6 or more and 14 or less carbon atoms or an ester compound thereof.

8. A transformant, wherein the transformant is a host organism transformed with a gene encoding the following protein (A) or (B),
wherein, as compared to the host organism that has not been transformed with the gene:
(a) expression of protein (A) or (B) is enhanced in the transformant;
and
(b) at least either (i) the productivity of medium-chain fatty acids or lipids containing the medium-chain fatty acids as components is increased, or (ii) the total amount of all fatty acids produced in a cell of the transformant is increased;
wherein protein (A) is:
(A) a protein consisting of the amino acid sequence set forth in SEQ ID NO: 1;
and
protein (B) is:
(B) a protein consisting of an amino acid sequence having 90% or more identity with the amino acid sequence of the protein (A), and having acyltransferase activity.

9. The transformant according to claim 8 , wherein expression of a gene encoding an acyl-ACP thioesterase having substrate specificity to a medium-chain acyl-ACP is enhanced, as compared to that of a transformant in which expression of the gene encoding the acyl-ACP thioesterase is not enhanced.

10. The transformant according to claim 8 , wherein the transformant is a microalga.

11. The transformant according to claim 10 , wherein the microalga is an alga belonging to the genus Nannochloropsis.

12. The method according to claim 2 , wherein expression of a gene encoding an acyl-ACP thioesterase having substrate specificity to a medium-chain acyl-ACP is enhanced in the transformant, as compared to that of a transformant in which expression of the gene encoding the acyl-ACP thioesterase is not enhanced.

13. The method according to claim 2 , wherein the transformant is a microalga.

14. The method according to claim 13 , wherein the microalga is an alga belonging to the genus Nannochloropsis.

15. The method according to claim 2 , wherein the lipids contain a fatty acid having 6 or more and 14 or less carbon atoms or an ester compound thereof.

16. The method according to claim 3 , wherein expression of a gene encoding an acyl-ACP thioesterase having substrate specificity to a medium-chain acyl-ACP is enhanced in the transformant, as compared to that of a transformant in which expression of the gene encoding the acyl-ACP thioesterase is not enhanced.

17. The method according to claim 3 , wherein the transformant is a microalga.

18. The method according to claim 17 , wherein the microalga is an alga belonging to the genus Nannochloropsis.

19. The method according to claim 3 , wherein the lipids contain a a fatty acid having 6 or more and 14 or less carbon atoms or an ester compound thereof.

20. The method according to claim 1 , wherein the gene encodes protein (A).

Assignees

• Kao Corp

Inventors

• Sugihara Shinji

Classifications

• C12N15/8247

  involving modified lipid metabolism, e.g. seed oil composition · CPC title

• C12Y301/02014

  Oleoyl-[acyl-carrier-protein] hydrolase (3.1.2.14), i.e. ACP-thioesterase · CPC title

• C12N9/1025

  Acyltransferases (2.3) · CPC title

• C07K14/405

  from algae · CPC title

• C12N9/16

  acting on ester bonds (3.1) · CPC title