Microorganisms and methods for the production of butadiene using acetyl-CoA

Patent metadata

Field	Value
Publication number	US-10487342-B2
Application number	US-201515325396-A
Country	US
Kind code	B2
Filing date	Jul 2, 2015
Priority date	Jul 11, 2014
Publication date	Nov 26, 2019
Grant date	Nov 26, 2019

Abstract

The invention provides non-naturally occurring microbial organisms containing butadiene or 2,4-pentadienoate pathways comprising at least one exogenous nucleic acid encoding a butadiene or 2,4-pentadienoate pathway enzyme expressed in a sufficient amount to produce butadiene or 2,4-pentadienoate. The organism can further contain a hydrogen synthesis pathway. The invention additionally provides methods of using such microbial organisms to produce butadiene or 2,4-pentadienoate by culturing a non-naturally occurring microbial organism containing butadiene or 2,4-pentadienoate pathways as described herein under conditions and for a sufficient period of time to produce butadiene or 2,4-pentadienoate. Hydrogen can be produced together with the production of butadiene or 2,4-pentadienoate.

First claim

What is claimed is:

1. A non-naturally occurring microbial organism, said microbial organism having a butadiene pathway and comprising at least four exogenous nucleic acids encoding butadiene pathway enzymes expressed in a sufficient amount to produce butadiene, wherein said butadiene pathway comprises a pathway selected from:
(1) 1A, 1B, 1C, 1G, 1I, 1L, 1M, and 1F;
(2) 1A, 1B, 1C, 1G, 1I, 1L, 1N, and 1F;
(3) 1A, 1B, 1C, 1H, 1I, 1L, 1M, and 1F;
(4) 1A, 1B, 1C, 1H, 1I, 1L, 1N, and 1F;
(5) 1A, 1B, 1C, 1D, 1J, 1L, 1M, and 1F;
(6) 1A, 1B, 1C, 1D, 1J, 1L, 1N, and 1F;
(7) 1A, 1B, 1C, 1D, 1K, 1L, 1M, and 1F; and
(8) 1A, 1B, 1C, 1D, 1K, 1L, 1N, and 1F,
wherein 1A is an acetaldehyde dehydrogenase, wherein 1B is a 4-hydroxy 2-oxovalerate aldolase, wherein 1C is a 4-hydroxy 2-oxovalerate dehydratase, wherein 1D is a 2-oxopentenoate reductase, wherein 1F is a 2,4-pentadienoate decarboxylase, wherein 1G is a 2-oxopentenoate ligase, wherein 1H is a 2-oxopentenoate: acetyl CoA transferase, wherein 1I is a 2-oxopentenoyl-CoA reductase, wherein 1J is a 2-hydroxypentenoate ligase, wherein 1K is a 2-hydroxypentenoate: acetyl-CoA CoA transferase, wherein 1L is a 2-hydroxypentenoyl-CoA dehydratase, wherein 1M is a 2,4-Pentadienoyl-CoA hydrolase, and wherein 1N is a 2,4-Pentadienoyl-CoA: acetyl CoA transferase.

2. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises four, five, six, seven, or eight, exogenous nucleic acids each encoding a butadiene pathway enzyme, or wherein said microbial organism comprises exogenous nucleic acids encoding each of the enzymes of at least one of the pathways selected from (1) to (8).

3. The non-naturally occurring microbial organism of claim 1 further comprising an acetyl-CoA pathway, wherein said acetyl-CoA pathway comprises a pathway selected from:
(1) 3T and 3V;
(2) 3T, 3W, and 3X;
(3) 3U and 3V; and
(4) 3U, 3W, and 3X,
wherein 3T is a fructose-6-phosphate phosphoketolase, wherein 3U is a xylulose-5-phosphate phosphoketolase, wherein 3V is a phosphotransacetylase, wherein 3W is an acetate kinase, wherein 3X is an acetyl-CoA transferase, an acetyl-CoA synthetase, or an acetyl-CoA ligase.

4. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism further comprises a formaldehyde fixation pathway, wherein said formaldehyde fixation pathway comprises:
(1) 3D and 3Z;
(2) 3D; or
(3) 3B and 3C,
wherein 3B is a 3-hexulose-6-phosphate synthase, wherein 3C is a 6-phospho-3-hexuloisomerase, wherein 3D is a dihydroxyacetone synthase, wherein 3Z is a fructose-6-phosphate aldolase.

5. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism further comprises a methanol metabolic pathway, wherein said methanol metabolic pathway comprises a pathway selected from:
(1) 4A and 4B;
(2) 4A, 4B and 4C;
(3) 4J;
(4) 4J, 4K and 4C;
(5) 4J, 4M, and 4N;
(6) 4J and 4L;
(7) 4J, 4L, and 4G;
(8) 4J, 4L, and 41;
(9) 4A, 4B, 4C, 4D, and 4E;
(10) 4A, 4B, 4C, 4D, and 4F;
(11) 4J, 4K, 4C, 4D, and 4E;
(12) 4J, 4K, 4C, 4D, and 4F;
(13) 4J, 4M, 4N, and 4O;
(14) 4A, 4B, 4C, 4D, 4E, and 4G;
(15) 4A, 4B, 4C, 4D, 4F, and 4G;
(16) 4J, 4K, 4C, 4D, 4E, and 4G;
(17) 4J, 4K, 4C, 4D, 4F, and 4G;
(18) 4J, 4M, 4N, 4O, and 4G;
(19) 4A, 4B, 4C, 4D, 4E, and 4I;
(20) 4A, 4B, 4C, 4D, 4F, and 4I;
(21) 4J, 4K, 4C, 4D, 4E, and 4I;
(22) 4J, 4K, 4C, 4D, 4F, and 4I; and
(23) 4J, 4M, 4N, 4O, and 41,
wherein 4A is a methanol methyltransferase, wherein 4B is a methylenetetrahydrofolate reductase, wherein 4C is a methylenetetrahydrofolate dehydrogenase, wherein 4D is a methenyltetrahydrofolate cyclohydrolase, wherein 4E is a formyltetrahydrofolate deformylase, wherein 4F is a formyltetrahydrofolate synthetase, wherein 4G is a formate hydrogen lyase, wherein 4I is a formate dehydrogenase, wherein 4J is a methanol dehydrogenase, wherein 4K is a formaldehyde activating enzyme or spontaneous, wherein 4L is a formaldehyde dehydrogenase, wherein 4M is a S-(hydroxymethyl)glutathione synthase or spontaneous, wherein 4N is a glutathione-dependent formaldehyde dehydrogenase, wherein 4O is a S-formylglutathione hydrolase.

6. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism further comprises a formate assimilation pathway, wherein said formate assimilation pathway comprises a pathway selected from:
(1) 3E;
(2) 3F, and 3G;
(3) 3H, 3I, 3J, and 3K;
(4) 3H, 3I, 3J, 3L, 3M, and 3N;
(5) 3E, 3H, 3I, 3J, 3L, 3M, and 3N;
(6) 3F, 3G, 3H, 3I, 3J, 3L, 3M, and 3N;
(7) 3K, 3H, 3I, 3J, 3L, 3M, and 3N; and
(8) 3H, 3I, 3J, 3O, and 3P,
wherein 3E is a formate reductase, 3F is a formate ligase, a formate transferase, or a formate synthetase, wherein 3G is a formyl-CoA reductase, wherein 3H is a formyltetrahydrofolate synthetase, wherein 31 is a methenyltetrahydrofolate cyclohydrolase, wherein 3J is a methylenetetrahydrofolate dehydrogenase, wherein 3K is a formaldehyde-forming enzyme or spontaneous, wherein 3L is a glycine cleavage system, wherein 3M is a serine hydroxymethyltransferase, wherein 3N is a serine deaminase, wherein 3O is a methylenetetrahydrofolate reductase, wherein 3P is an acetyl-CoA synthase.

7. The non-naturally occurring microbial organism of claim 6 , wherein said formate assimilation pathway further comprises:
(1) 3Q;
(2) 3R and 3S;
(3) 3Y and 3Q; or
(4) 3Y, 3R, and 3S,
wherein 3Q is a pyruvate formate lyase, wherein 3R is a pyruvate dehydrogenase, a pyruvate ferredoxin oxidoreductase, or a pyruvate:NADP+oxidoreductase, wherein 3S is a formate dehydrogenase, wherein 3Y is a glyceraldehyde-3-phosphate dehydrogenase or an enzyme of lower glycolysis.

8. The non-naturally occurring microbial organism of claim 1 , wherein said organism further comprises:
(a) a methanol oxidation pathway, wherein said methanol oxidation pathway comprises 3A, wherein 3A a methanol dehydrogenase;
(b) a carbon monoxide dehydrogenase;
(c) a hydrogenase;
(d) attenuation of one or more endogenous enzymes selected from DHA kinase, methanol oxidase, PQQ-dependent methanol dehydrogenase, DHA synthase or any combination thereof;
(e) attenuation of one or more endogenous enzymes of a competing formaldehyde assimilation or dissimilation pathway;
(f) a gene disruption of one or more endogenous nucleic acids encoding enzymes selected from DHA kinase, methanol oxidase, PQQ-dependent methanol dehydrogenase, DHA synthase or any combination thereof;
(g) a gene disruption of one or more endogenous nucleic acids encoding enzymes of a competing formaldehyde assimilation or dissimilation pathway; or
(h) a hydrogen synthesis pathway catalyzing the synthesis of hydrogen from a reducing equivalent, said hydrogen synthesis pathway comprising an enzyme selected from the group consisting of a hydrogenase, a formate-hydrogene lyase and ferredoxin: NADP+ oxidoreductase.

9. The non-naturally occurring microbial organism of claim 1 , wherein said at least one exogenous nucleic acid is a heterologous nucleic acid.

10. The non-naturally occurring microbial organism of claim 1 , wherein said non-naturally occurring microbial organism is in a substantially anaerobic culture medium.

11. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism is a species of bacteria, yeast, or fungus.

12. The non-naturally occurring microbial organism of claim 1 , wherein said butadiene pathway comprises 1A, 1B, 1C, 1G, 1I, 1L, 1M, and 1F.

13. The non-naturally occurring microbial organism of claim 1 , wherein said butadiene pathway comprises 1A, 1B, 1C, 1G, 1I, 1L, 1N, and 1F.

Assignees

• Genomatica Inc

Inventors

• Pharkya Priti
• Burgard Anthony P
• Burk Mark J

Classifications

• C12N9/12

  transferring phosphorus containing groups, e.g. kinases (2.7) · CPC title

• C12P7/40

  containing a carboxyl group {including Peroxycarboxylic acids} · CPC title

• C12N9/0006

  acting on CH-OH groups as donors (1.1) · CPC title

• C12Y101/03013

  Alcohol oxidase (1.1.3.13) · CPC title

• C12N9/10

  Transferases (2.) (ribonucleases C12N9/22) · CPC title