Antimicrobial peptide for nosocomial infections

Patent metadata

Field	Value
Publication number	US-10487117-B2
Application number	US-201615747934-A
Country	US
Kind code	B2
Filing date	Jul 28, 2016
Priority date	Jul 30, 2015
Publication date	Nov 26, 2019
Grant date	Nov 26, 2019

Abstract

A novel antimicrobial peptide is provided having an inhibitory effect on biofilms produced by biofilm-forming bacteria. The peptide is effective against wide range bacterial targets; generates no antimicrobial resistance; agglutinates bacteria cells enhancing the pathogen removal at the infectious focus; promotes biofilm eradication and prevents biofilm development. Compositions comprising the peptide are provided to treat and prevent infectious diseases, and for other uses such as the disinfection of medical and surgical materials.

First claim

The invention claimed is:

1. A peptide consisting of the amino acid sequence:

X 1 -Pro 2 -X 3 -X 4 -X 5 -Ala 6 -Gln 7 -Trp 8 -Phe 9 -Ala 10 -Ile 11 -Gln 12 -

His 13 -Ile 14 -X 15 -Pro 16 -X 17 -X 18 -X 19 -Ala 20 -Met 21 -X 22 -Ala 23 -

Ile 24 -X 25 -X 26 -Tyr 27 -Arg 28 -Trp 29 -Arg 30 -X 31 -NH2,

wherein:
X 1 , X 5 , X 17 , X 22 are amino acids selected from the group consisting of Arg and Lys;
X 3 is an amino acid selected from the group consisting of Phe, Leu, Ile and Val;
X 4 is an amino acid selected from the group consisting of Thr, Phe and Ile;
X 15 is any amino acid;
X 18 is an amino acid selected from the group consisting of Thr, Ser, Gln and Asn;
X 19 is an amino acid selected from the group consisting of Ile, Leu and Val;
X 25 is an amino acid selected from the group consisting of Asn, His, and Lys;
X 26 is an amino acid selected from the group consisting of Asn, Lys and Trp; and
X 31 is either absent or from one to nine amino acids selected from the group consisting of Asn, Gln, Ser and Thr.

2. The peptide according to claim 1 , wherein the peptide has antimicrobial activity and has an inhibitory effect on biofilm formation by a biofilm-producing microorganism.

3. A peptide consisting of an amino acid sequence having a 90% identity to amino acid sequence SEQ ID NO: 1, wherein the peptide has antimicrobial activity and has an inhibitory effect on biofilm formation by a biofilm-producing microorganism.

4. The peptide according to claim 3 , wherein the amino acid sequence is SEQ ID NO: 1.

5. An antimicrobial agent comprising the peptide according to claim 1 .

6. An antibiofilm agent comprising the peptide according to claim 1 .

7. A disinfectant for medical and surgical materials, the disinfectant comprising the peptide according to claim 1 .

8. A medicament comprising the peptide according to claim 1 .

9. A method for the treatment of an infectious disease in a subject in need thereof, the method comprising: administering an effective amount of the medicament according to claim 8 to the subject.

10. The method according to claim 9 , wherein the infectious disease is selected from the group consisting of a bacterial, a viral, a parasitic and a fungal disease.

11. The method according to claim 10 , wherein the infectious disease is a bacterial disease and is caused by a Gram negative bacterial pathogen.

12. The method according to claim 11 , wherein the Gram negative pathogen is selected from the group consisting of: Pseudomonas, Escherichia coli, Acinetobacter, Salmonella and Klebsiella.

13. A pharmaceutical composition comprising:
the peptide according to claim 1 ; and
a pharmaceutically acceptable carrier, diluent or excipient.

14. A composition comprising,
the peptide according to claim 1 ; and
a carrier or diluent.

15. An item impregnated with, coated with or covered by a composition comprising the peptide according to claim 1 , wherein the item is selected from the group consisting of a medical device, a medical instrument, a medical implement, a prosthetic, an implantable device or material, a wound dressing, and a biologically compatible material.

Assignees

• Univ Barcelona Autonoma
• Univ Pompeu Fabra

Inventors

• Boix Borrás Ester
• Torrent Burgas Marc
• Andreu David

Classifications

• C07K14/001 Primary

  by chemical synthesis · CPC title

• A61K38/00

  Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) · CPC title

• A61P31/04

  Antibacterial agents · CPC title

• Y02A50/473

  Cross-Sectional Technologies · mapped topic

• Y02A50/481

  Cross-Sectional Technologies · mapped topic