Diagnostic method and biomarker for lifestyle disease

Patent metadata

Field	Value
Publication number	US-10481169-B2
Application number	US-201815906032-A
Country	US
Kind code	B2
Filing date	Feb 27, 2018
Priority date	Feb 28, 2017
Publication date	Nov 19, 2019
Grant date	Nov 19, 2019

Abstract

Provided is a novel method and the like for diagnosing a lifestyle disease. The method for diagnosing a lifestyle disease provided by the present invention includes: collecting a biological sample from a subject; measuring the concentration of a fatty acid amide contained in the biological sample; determining whether the subject suffers from a lifestyle disease or a lifestyle disease has progressed when the measured value of the concentration of the fatty acid amide in the sample obtained from the subject is lower than a measurement result obtained from a healthy subject.

First claim

What is claimed is:

1. A method for diagnosing a lifestyle disease comprising:
preparing a measurement result of a concentration of a fatty acid amide obtained from a healthy subject;
collecting a biological sample from a subject;
measuring a concentration of the fatty acid amide contained in the biological sample; and
determining whether the subject suffers from a lifestyle disease or a lifestyle disease has progressed when a measured value of the concentration of the fatty acid amide obtained from the subject is lower than the measurement result obtained from the healthy subject.

2. The method for diagnosing a lifestyle disease according to claim 1 , wherein the biological sample is an organ or blood.

3. The method for diagnosing a lifestyle disease according to claim 1 , wherein the concentration of the fatty acid amide is measured by liquid chromatography-mass spectrometry.

4. The method for diagnosing a lifestyle disease according to claim 3 , wherein the liquid chromatography-mass spectrometry includes
isolating two or more components contained in the biological sample by liquid chromatography,
ionizing the isolated components and selecting a specific mass component with a quadrupole mass spectrometer, then dissociating the specific mass component, and
detecting the dissociated component with a mass spectrometer.

5. The method for diagnosing a lifestyle disease according to claim 1 , wherein the lifestyle disease is at least one selected from the group consisting of obesity, dyslipidemia, diabetes and impaired glucose tolerance.

6. The method for diagnosing a lifestyle disease according to claim 1 , wherein the fatty acid amide is oleamide.

Assignees

• Toyota Motor Co Ltd
• Univ Nagoya Nat Univ Corp

Inventors

• Uchida Koji
• Shibata Takahiro
• Ishikawa Kenji
• LI Oi Lun Helena
• Matsushita Tadashi
• Kikuchi Ryosuke
• Ukai Junzo

Classifications

• G01N2800/042

  Disorders of carbohydrate metabolism, e.g. diabetes, glucose metabolism · CPC title

• H01J49/0027

  Methods for using particle spectrometers · CPC title

• G01N2560/00

  Chemical aspects of mass spectrometric analysis of biological material · CPC title

• G01N2800/044

  Hyperlipemia or hypolipemia, e.g. dyslipidaemia, obesity · CPC title

• H01J49/165

  Electrospray ionisation · CPC title