Electrolyte-gated transistors for detection of molecules

Patent metadata

Field	Value
Publication number	US-10481126-B2
Application number	US-201515503277-A
Country	US
Kind code	B2
Filing date	Aug 13, 2015
Priority date	Aug 14, 2014
Publication date	Nov 19, 2019
Grant date	Nov 19, 2019

Abstract

The disclosure describes methods, devices, and system that measure chemisorption potentiometrically for detection of target molecules. In one example, a device includes a semiconductor, an ionic conducting electronic insulator coupled to the semiconductor, a floating gate electrode comprising a first portion and a second portion, the first portion being coupled to the semiconductor via the ionic conducting electronic insulator, an aqueous buffer, and a primary gate electrode coupled to the second portion of the floating gate electrode via the aqueous buffer. The second portion of the floating gate electrode may comprise a probe configured to react with a target chemical composition of a molecule to detect the presence of the molecule. Reaction with the target chemical composition may change an electrical property of the device and indicate the presence of the molecule in the aqueous buffer.

First claim

What is claimed is:

1. A device comprising:
a semiconductor;
an ionic conducting electronic insulator coupled to the semiconductor;
a floating gate electrode comprising a first portion and a second portion, the first portion being coupled to the semiconductor via the ionic conducting electronic insulator;
an aqueous buffer; and
a primary gate electrode coupled to the second portion of the floating gate electrode via the aqueous buffer.

2. The device of claim 1 , wherein the semiconductor comprises an organic semiconductor.

3. The device of claim 1 , wherein the ionic conducting electronic insulator comprises a plurality of electrolyte dielectrics.

4. The device of claim 3 , wherein the plurality of electrolyte dielectrics insulates one or more surfaces of the floating gate electrode.

5. The device of claim 1 , wherein the second portion of the floating gate electrode comprises a surface chemically bound to a plurality of probe molecules.

6. The device of claim 5 , wherein each probe molecule of the plurality of probe molecules comprises a first portion and a second portion, the first portion of each probe molecule being chemically bound to the surface of the floating gate electrode, and the second portion of each probe molecule comprising an affinity reagent configured to bind to a target chemical composition.

7. The device of claim 6 , wherein the affinity reagent comprises at least one of a nucleic acid, a nucleic acid analog, a polypeptide, a protein, and an antibody.

8. The device of claim 5 , wherein the surface of the second portion of the floating gate electrode comprises the plurality of probe molecules and a plurality of blocking molecules, the blocking molecules configured to prevent non-specific adsorption.

9. The device of claim 8 , wherein the surface is chemically bound to the plurality of blocking molecules, the plurality of blocking molecules having a greater chemical affinity for the surface of the second portion of the floating gate electrode than unreacted probe molecules and displacing the unreacted probe molecules from the surface of the second portion of the floating gate.

10. The device of claim 8 , wherein the blocking molecules comprise a chemical formula of HS(CH 2 ) 6 OH.

11. The device of claim 1 , wherein the second portion of the floating gate electrode comprises a surface functionalized with a self-assembled monolayer of molecules.

12. The device of claim 11 , wherein the self-assembled monolayer of molecules comprises molecules selected from the group of molecules comprising 6-mercapto-1-hexanol and 3,3,4,4,5,5,6,6,6-nonaflouro-1-hexanol.

13. The device of claim 1 , wherein the primary gate electrode is in direct contact with the aqueous buffer to capacitively couple the primary gate electrode to the second portion of the floating gate electrode via the aqueous buffer.

14. The device of claim 1 , wherein the device comprises a transistor, the transistor comprising the semiconductor, the ionic conducting electronic insulator, the floating gate electrode, the aqueous buffer; and the primary gate electrode.

15. A system for detecting a molecule having a target chemical composition, the system comprising:
a semiconductor;
an ionic conducting electronic insulator coupled to the semiconductor;
a floating gate electrode comprising a first portion and a second portion, the first portion being coupled to the semiconductor via the ionic conducting electronic insulator;
an aqueous buffer;
a primary gate electrode coupled to the second portion of the floating gate electrode via the aqueous buffer; and
one or more circuits configured to measure an electrical property based on a voltage change over the ionic conducting electronic insulator, and output an indication of the electrical property.

16. The system of claim 15 , wherein the semiconductor comprises an organic semiconductor.

17. The system of claim 15 , wherein the ionic conducting electronic insulator comprises a plurality of electrolyte dielectrics.

18. The system of claim 17 , wherein the plurality of electrolyte dielectrics insulates one or more surfaces of the floating gate electrode.

19. The system of claim 15 , wherein the second portion of the floating gate electrode comprises a surface chemically bound to a plurality of probe molecules.

20. The system of claim 19 , wherein each probe molecule of the plurality of probe molecules comprises a first portion and a second portion, the first portion of each probe molecule being chemically bound to the surface of the second portion of the floating gate electrode, and the second portion of each probe molecule comprising an affinity reagent configured to bind to the target chemical composition.

21. The system of claim 20 , wherein the affinity reagent comprises at least one of a nucleic acid, a nucleic acid analog, a polypeptide, a protein, and an antibody.

22. The system of claim 19 , wherein the surface of the second portion of the floating gate electrode comprises the plurality of probe molecules and a plurality of blocking molecules, the blocking molecules configured to prevent non-specific adsorption.

23. The system of claim 15 , wherein the primary gate electrode is in direct contact with the aqueous buffer to capacitively couple the primary gate electrode to the second portion of the floating gate electrode via the aqueous buffer.

24. A method for detecting a molecule comprising a target chemical composition, the method comprising:
applying a voltage to a primary gate electrode of a device, the device comprising:
a source electrode and a drain electrode, the source electrode and the drain electrode being coupled to a semiconductor;
an ionic conducting electronic insulator coupled to the semiconductor;
a floating gate electrode comprising a first portion and a second portion, the first portion being coupled to the semiconductor via the ionic conducting electronic insulator; and
an aqueous buffer, wherein the primary gate electrode is coupled to the second portion of the floating gate electrode via the aqueous buffer;

measuring an electrical property at the drain electrode;
determining, based on the measured electrical property, whether the target chemical composition of the molecule is present within the aqueous buffer; and
outputting an indication of the determination.

25. The method of claim 24 , wherein the ionic conducting electronic insulator comprises a plurality of electrolyte dielectrics.

26. The method of claim 25 , wherein the plurality of electrolyte dielectrics insulates one or more surfaces of the floating gate electrode.

27. The method of claim 24 , wherein the second portion of the floating gate electrode comprises a surface chemically bound to a plurality of probe molecules.

28. The method of claim 27 , wherein each probe molecule of the plurality of probe molecules comprises a first portion and a second portion, the first portion of each probe molecule being chemically bound to the surface of the second portion of the floating gate electrode, and the second portion of each probe molecule comprising an affinity reagent configured to bind to the target chemical composition.

29. The method of claim 28 , wherein the affinity reagent comprises at least one of a nucleic acid, a nucleic acid analog, a polypeptide, a protein, and an antibody.

30. The method of

Assignees

• Univ Minnesota

Inventors

• White Scott P
• Frisbie C Daniel
• Dorfman Kevin D

Classifications

• G01N27/4161

  measuring the voltage and using a constant current supply, e.g. chronopotentiometry · CPC title

• G01N27/4145 Primary

  specially adapted for biomolecules, e.g. gate electrode with immobilised receptors · CPC title

• C12Q1/6869

  Methods for sequencing · CPC title

• G01N33/48721

  Investigating individual macromolecules, e.g. by translocation through nanopores (Coulter counters in general G01N15/12; fabrication methods for nanoscale apertures B81B1/00; sequencing of nucleic acids C12Q1/68) · CPC title

• H01L51/052

  Electricity · mapped topic