Device and method for observing and filter for capturing a minute substance

Patent metadata

Field	Value
Publication number	US-10478784-B2
Application number	US-201715682715-A
Country	US
Kind code	B2
Filing date	Aug 22, 2017
Priority date	Feb 26, 2015
Publication date	Nov 19, 2019
Grant date	Nov 19, 2019

Abstract

There is provided a glass substrate for observing minute substance, made of porous glass and capable of separating and capturing a minute substance with a 10 to 500 nm average particle diameter contained in a solution or a suspension, comprising a porous glass substrate having a plurality of pores, wherein the plurality of pores has an average pore diameter ranging from 30 to 110% of the average particle diameter of the minute substance, each of the plurality of pores has a surface pore diameter on an uppermost surface of the glass substrate, a standard deviation of the surface pore diameter is 60% or less of the average particle diameter of the minute substance, and a pore with a pore diameter ranging from 60 to 140% of a pore diameter at peak top in a pore diameter distribution of the plurality of pores occupies 90% or more of total pore volume.

First claim

What is claimed is:

1. A filter for capturing a minute substance, comprising a porous glass substrate having a plurality of pores,
wherein
the porous glass substrate comprises phase-separated glass formed through a spinodal decomposition,
the plurality of pores has an average pore diameter ranging from 5 to 2500 nm and a pore diameter ranging from 60 to 140% of a pore diameter corresponding to a maximum value in a pore diameter distribution of the plurality of pores occupies 90% or more of total pore volume of the plurality of pores, and
the porous glass substrate satisfies the following formula (1):

S

D
·

log
10

⁢
d

≤

4.5
⁡

[

cm
2

/
mm

]

(
1
)

wherein S [cm 2 ] is an area of main surface of the glass substrate,
D [mm] is a plate thickness of the porous glass substrate, and
d [nm] is the average pore diameter.

2. The filter for capturing a minute substance according to claim 1 , wherein the porous glass substrate has a porosity ranging from 20 to 90%.

3. A filter for capturing a minute substance, comprising a porous glass substrate having a plurality of pores,
wherein
the plurality of pores has an average pore diameter ranging from 5 to 2500 nm and a pore diameter ranging from 60 to 140% of a pore diameter corresponding to a maximum value in a pore diameter distribution of the plurality of pores occupies 90% or more of total pore volume of the plurality of pores, and
the porous glass substrate has a covering layer on a surface, the covering layer comprising a protein anti-adhesion agent.

4. A minute substance observation device comprising:
a capturing unit including the filter according to claim 1 and being capable of separating and capturing a minute substance with an average particle diameter of 10 to 500 nm contained in a solution or a suspension; and
an observing unit for observing the minute substance captured on a surface of the glass substrate.

5. The minute substance observation device according to claim 4 , wherein the observing unit is a fluorescence microscope.

6. A minute substance capturing method comprising:
introducing a solution or a suspension comprising a minute substance with a 10 to 500 nm average particle diameter to a porous glass substrate having a plurality of pores; and
separating and capturing the minute substance by the glass substrate,
wherein
the porous glass substrate comprises phase-separated glass formed through a spinodal decomposition,
the plurality of pores has an average pore diameter ranging from 30 to 500% of the average particle diameter of the minute substance, and a pore diameter ranging from 60 to 140% of a pore diameter corresponding to a maximum value in a pore diameter distribution of the plurality of pores occupies 90% or more of total pore volume of the plurality of pores.

7. The minute substance capturing method according to claim 6 , wherein the porous glass substrate satisfies the following formula (1):

S

D
·

log
10

⁢
d

≤

4.5
⁡

[

cm
2

/
mm

]

,

(
1
)

wherein
S [cm 2 ] is an area of main surface of the glass substrate,
D [mm] is a plate thickness of the porous glass substrate, and
d [nm] is the average pore diameter.

8. A minute substance observation method comprising:
introducing a solution or a suspension comprising a minute substance with a 10 to 500 nm average particle diameter to a porous glass substrate having a plurality of pores;
separating and capturing the minute substance by the glass substrate; and
observing the minute substance captured by the glass substrate,
wherein
an average pore diameter ranging from 30 to 110% of the average particle diameter of the minute substance,
each of the plurality of pores has a surface pore diameter on a main surface of the porous glass substrate, and a standard deviation of the surface pore diameter on an uppermost surface of the glass substrate is 60% or less of the average particle diameter of the minute substance, and
a pore with a pore diameter ranging from 60 to 140% of a pore diameter corresponding to a maximum value in a pore diameter distribution of the plurality of pores occupies 90% or more of total pore volume of the plurality of pores.

9. The minute substance observation method according to claim 8 , wherein the average pore diameter is ranging from 30 to 100% of the average particle diameter of the minute substance.

10. A minute substance observation method comprising:
separating and capturing a minute substance with a 10 to 500 nm average particle diameter by the filter according to claim 1 , from a solution or a suspension comprising the minute substance; and
observing the minute substance captured on a surface of the glass substrate for observing minute substance.

11. The minute substance observation method according to claim 8 , wherein a fluorescence microscope is used for the observation.

Assignees

• Agc Inc

Inventors

• Yamazaki Shuji
• Kimura Shuichiro
• Kitaoka Kenji
• Hanawa Yu

Classifications

• G02B21/0076

  arrangements using fluorescence or luminescence · CPC title

• C03C11/005

  obtained by leaching after a phase separation step · CPC title

• C03C3/089

  containing boron · CPC title

• G01N2001/4088

  filtration · CPC title

• G01N1/4077

  by other techniques involving separation of suspended solids · CPC title