Skin substitutes and methods for hair follicle neogenesis

Patent metadata

Field	Value
Publication number	US-10478526-B2
Application number	US-201414888613-A
Country	US
Kind code	B2
Filing date	May 1, 2014
Priority date	May 3, 2013
Publication date	Nov 19, 2019
Grant date	Nov 19, 2019

Abstract

This invention provides compositions in the form of skin substitutes comprising epithelial cells and mesenchymal cells, wherein the mesenchymal cells are not isolated from the occipital or nape region of the scalp, as well as methods for using the same.

First claim

What is claimed is:

1. A skin substitute comprising, in a suspension,
(i) epithelial cells; and
(ii) isolated mesenchymal cells that are derived from the temporal region of a scalp, wherein the skin substitute induces hair follicle neogenesis.

2. The skin substitute of claim 1 , wherein the skin substitute is provided in a microsphere.

3. The skin substitute of claim 1 , wherein the suspension comprises a matrix.

4. The skin substitute of claim 3 , wherein the matrix is a collagen matrix.

5. The skin substitute of claim 1 , wherein the isolated mesenchymal cells are hair follicle dermal cells.

6. The skin substitute of claim 5 , wherein the hair follicle dermal cells are neural crest-derived cells, dermal papilla cells, or dermal sheath cells.

7. The skin substitute of claim 1 , wherein the epithelial cells are keratinocytes.

8. The skin substitute of claim 1 , wherein the epithelial cells are from a first or second passage and/or the isolated mesenchymal cells are from a first, second, third, or fourth passage.

9. The skin substitute of claim 1 , wherein the epithelial cells and the isolated mesenchymal cells are passaged in keratinocyte-conditioned medium.

10. The skin substitute of claim 1 , wherein the epithelial cells and the isolated mesenchymal cells are human.

11. The skin substitute of claim 1 , wherein the isolated mesenchymal cells are genetically unmodified mesenchymal cells.

12. A method for inducing hair follicle neogenesis, comprising delivering the skin substitute of claim 1 to a human subject, wherein the human subject has partial-thickness skin loss, full-thickness skin loss, a wound, a burn, a scar, or hair loss.

Assignees

• Henry M Jackson Found Advancement Military Medicine Inc

Inventors

• Thangapazham Rajesh
• Darling Thomas N
• Li Shaowei

Classifications

• A61P17/14

  for baldness or alopecia · CPC title

• A61P17/02

  for treating wounds, ulcers, burns, scars, keloids, or the like · CPC title

• A61P17/00

  Drugs for dermatological disorders · CPC title

• A61L27/60

  Materials for use in artificial skin · CPC title

• A61L27/3813

  Epithelial cells, e.g. keratinocytes, urothelial cells · CPC title