System for analysing a transparent sample with control of position, and associated method

Patent metadata

Field	Value
Publication number	US-10473576-B2
Application number	US-201515540068-A
Country	US
Kind code	B2
Filing date	Oct 23, 2015
Priority date	Dec 30, 2014
Publication date	Nov 12, 2019
Grant date	Nov 12, 2019

Abstract

A system for analyzing a transparent particle including: an analysis pathway, including a first light source emitting an analysis light beam, and a first optical system focusing the analysis light beam in a focusing plane; and a position control pathway including a second light source, an image sensor, and a second optical system at least partially merged with the first optical system. The image sensor is offset relative to the image of the focusing plane by the second optical system. The system makes it possible to control correct positioning of the particle, even though it is transparent, and without disturbing the analysis pathway.

First claim

The invention claimed is:

1. A system for analyzing a particle of interest of a biological sample, comprising:
an analysis pathway, comprising a first light source emitting an analysis light beam, and a first optical system configured to focus the analysis light beam in a plane orthogonal to the optical axis thereof, as a focusing plane;
a position control pathway, comprising a second light source, an image sensor, and a second optical system at least partially merged with the first optical system such that the first optical system and the second optical system comprise a shared optical system; and
computing means configured to compute, by numerical propagation, at least one image, reconstructed from an image provided by the image sensor;
wherein the image sensor is offset relative to an image of the focusing plane by the second optical systems;
wherein the computing means receives at an input thereto an image provided by the image sensor, and is configured to compute, based on the image provided by the image sensor and by numerical propagation, a series of reconstructed images in reconstruction planes, each of which is offset relative to the plane of the image sensor; and
wherein the computing means comprises means for determining, based on the series of reconstructed images, an offset relative to the plane of the image sensor, as an optimum offset, associated with a minimum offset between the reconstruction plane and a plane receiving an image of the particle of interest by the second optical system.

2. A system according to claim 1 , wherein the computing means receives at an input thereto an image provided by the image sensor, and is configured to compute, based on the image provided by the image sensor and by numerical propagation, a reconstructed image of the particle of interest in the image of the focusing plane.

3. A system according to claim 1 , wherein the computing means comprises means for comparing the optimum offset with the distance between the plane of the image sensor and the image of the focusing plane by the second optical system, a result of the comparison determining an axial displacement command, and further comprising displacement means configured to move a support for receiving the sample, in response to the axial displacement command.

4. A system according to claim 1 , further comprising partial and/or selective deflection means, to combine the position control pathway and the analysis pathway at an input to the shared optical system.

5. A system according to claim 4 , wherein the first light source and the second light source have different wavelengths, and the deflection means is wavelength-selective means.

6. A method for analyzing a particle of interest of a biological sample, implemented within a system according to claim 1 , comprising:
analyzing the particle of interest using the analysis pathway; and
controlling the position of the particle of interest using the position control pathway;
wherein implementation of the analyzing and controlling is not separated by an intermediary of moving the sample relative to the analysis and position control pathways;
wherein the controlling the position comprises:
acquiring an image of the particle of interest using the image sensor of the position control pathway;
using the image to construct, by numerical propagation, at least one reconstructed image, in a reconstruction plane that is offset relative to the plane of the image sensor;

the method further comprising a computing a series of reconstructed images in reconstruction planes, each of which is offset relative to the plane of the image sensor, and selecting the reconstructed image associated with the minimum offset between the corresponding reconstruction plane and a plane receiving the image of the particle by the second optical system.

7. A method according to claim 6 , wherein the analyzing and controlling are implemented simultaneously.

8. A method according to claim 6 , further comprising computing a reconstructed image of the particle of interest in the image of the focusing plane.

9. A system for analyzing a particle of interest of a biological sample, comprising:
an analysis pathway, comprising a first light source emitting an analysis light beam, and a first optical system configured to focus the analysis light beam in a plane orthogonal to the optical axis thereof, as a focusing plane;
a position control pathway, comprising a second light source, an image sensor, and a second optical system at least partially merged with the first optical system such that the first optical system and the second optical system comprise a shared optical system; and
computing means configured to compute, by numerical propagation, at least one image, reconstructed from an image provided by the image sensor;
wherein the image sensor is offset relative to an image of the focusing plane by the second optical system;
wherein the computing means comprises means for comparing a control image with a focused reconstructed image, the focused image being associated with a minimum offset between a position of a corresponding reconstruction plane, and a plane receiving an image of the particle of interest by the second optical system.

10. A system according to claim 9 , wherein a result of the comparison between the control image and the focused image determines a transverse displacement command, and further comprising displacement means configured to move a support for receiving the sample, in response to the transverse displacement command.

11. A system for analyzing a particle of interest of a biological sample, comprising:
an analysis pathway, comprising a first light source emitting an analysis light beam, and a first optical system configured to focus the analysis light beam in a plane orthogonal to the optical axis thereof, as a focusing plane;
a position control pathway, comprising a second light source, an image sensor, and a second optical system at least partially merged with the first optical system such that the first optical system and the second optical system comprise a shared optical system;
computing means configured to compute, by numerical propagation, at least one image, reconstructed from an image provided by the image sensor; and
means for thresholding a reconstructed image, the threshold being defined by i th =i av +3σ wherein i av is mean value of intensity of the pixels in the reconstructed image and σ is standard deviation of the intensity of the pixels in the reconstructed image;
wherein the image sensor is offset relative to an image of the focusing plane by the second optical system.

Assignees

• Commissariat Energie Atomique
• Biomerieux Sa

Inventors

• Perraut Francois
• Joly Pierre
• Josso Quentin
• Kloster-Landsberg Meike

Classifications

• G03H2001/0469

  Object light being reflected by the object · CPC title

• G03H2001/005

  in microscopy, e.g. digital holographic microscope [DHM] · CPC title

• G03H1/0866

  Digital holographic imaging, i.e. synthesizing holobjects from holograms · CPC title

• G03H2001/0883

  Reconstruction aspect, e.g. numerical focusing · CPC title

• G03H2001/0428

  Image holography, i.e. an image of the object or holobject is recorded (G03H1/0406 takes precedence; holographic microscope G03H2001/005) · CPC title