Methods and means for modulating flowering time in monocot plants

Patent metadata

Field	Value
Publication number	US-10472645-B2
Application number	US-201414902317-A
Country	US
Kind code	B2
Filing date	Jul 1, 2014
Priority date	Jul 1, 2013
Publication date	Nov 12, 2019
Grant date	Nov 12, 2019

Abstract

The present invention relates to the identification of monocot FLC sequences, such as wheat FLC sequences, as well as their uses in modulating flowering time, seed development and seed germination.

First claim

The invention claimed is:

1. A method for accelerating temperature dependent flowering in a monocot plant comprising introducing a nucleic acid molecule into said plant and decreasing the expression and/or activity of an Flowering Locus C (FLC) gene and/or FLC protein in said plant or plant part, plant organ or plant cell of said plant, wherein the coding sequence of said FLC gene comprises a nucleotide sequence having at least 90% sequence identity to any one of SEQ ID NOs: 1, 7 or 17, or wherein said FLC protein comprises an amino acid sequence having at least 90% sequence identity to any one of SEQ ID NOs: 2, 8 or 18, wherein accelerated flowering in said monocot plant is compared to flowering of a control plant wherein said expression and/or activity of said FLC gene or said FLC protein has not been decreased.

2. The method according to claim 1 , wherein said decreasing the expression of and/or activity of an FLC gene and/or protein comprises expressing in said plant, plant part, plant organ or plant cell a chimeric gene comprising the following operably linked elements:
i) a plant-expressible promoter,
ii) a nucleic acid which when transcribed results in a decreased expression and/or activity of an endogenous FLC gene and/or protein in said monocot plant, plant part, plant organ or plant cell, and
iii) optionally, a 3′ end region involved in transcription termination and polyadenylation functional in plants.

3. The method according to claim 1 , wherein said monocot plant is a cereal plant.

4. The method according to claim 3 , wherein said cereal plant is a temperate cereal plant.

5. The method according to claim 1 , wherein said monocot plant is corn, barley, wheat, spelt, rye, or oats.

6. The method according to claim 1 , wherein said monocot plant is wheat.

7. The method according to claim 1 , wherein said monocot plant is a winter variety of wheat or barley.

8. The method according to claim 1 , wherein said FLC gene comprises a nucleotide sequence having at least 95% sequence identity to any one of SEQ ID NOs: 1, 7 or 17, or wherein said FLC protein comprises an amino acid sequence having at least 95% sequence identity to any one of SEQ ID NOs: 2, 8 or 18.

9. The method according to claim 8 , wherein said monocot plant is selected from corn, barley, wheat, spelt, rye, or oats.

10. The method according to claim 8 , wherein said monocot plant is wheat.

11. The method according to claim 8 , wherein said monocot plant is a winter variety of wheat or barley.

12. The method according to claim 1 , wherein said FLC gene comprises a nucleotide sequence of any one of SEQ ID NOs: 1, 7 or 17, or wherein said FLC protein comprises an amino acid sequence of any one of SEQ ID NOs: 2, 8 or 18.

Assignees

• Bayer Cropscience Nv
• Bayer Cropscience Lp
• Basf Se

Inventors

• Geuten Koen
• Kaufmann Kerstin
• Ruelens Philip

Classifications

• C07K14/4703

  Inhibitors; Suppressors · CPC title

• C07K14/415

  from plants · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12N15/827 Primary

  Flower development or morphology, e.g. flowering promoting factor [FPF] · CPC title

• C12Q2600/158

  Expression markers · CPC title