Methods and materials for biosynthesis of mogroside compounds

Patent metadata

Field	Value
Publication number	US-10465222-B2
Application number	US-201815974429-A
Country	US
Kind code	B2
Filing date	May 8, 2018
Priority date	Dec 4, 2012
Publication date	Nov 5, 2019
Grant date	Nov 5, 2019

Abstract

The invention relates to methods for producing mogrosides with the aid of enzymes. In particular the invention proposes various biosynthetic pathways useful for mogroside production and enzymes useful for mogroside production are provided. Furthermore, the invention provides recombinant hosts useful in performing the methods of the invention.

First claim

The invention claimed is:

1. A method of producing a mogrol precursor, a mogroside precursor, and/or a mogroside compound in a recombinant host cell comprising:
(a) a gene encoding a polypeptide capable of catalyzing conversion of oxido-squalene to produce cucurbitadienol;
wherein the polypeptide comprises a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NOs:1 or 43;

(b) a gene encoding a polypeptide capable of catalyzing conversion of dioxido-squalene to produce 24,25 epoxy cucurbitadienol;
wherein the polypeptide comprises a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NOs:1 or 43;

(c) a gene encoding a polypeptide capable of catalyzing hydroxylation of cucurbitadienol to produce 11-hydroxy-cucurbitadienol;
wherein the polypeptide comprises a polypeptide having at least 90% sequence identity to the amino acid sequence encoded by a nucleotide sequence set forth in any one of SEQ ID NOs:3-20, or 41 or the amino acid sequence set forth in SEQ ID NO:44;

(d) a gene encoding a polypeptide capable of catalyzing conversion of 11-hydroxy-cucurbitadienol to produce mogrol;
wherein the polypeptide comprises a polypeptide having at least 90% sequence identity to the amino acid sequence encoded by a nucleotide sequence set forth in SEQ ID NOs:38 or 40;

(e) a gene encoding a polypeptide capable of catalyzing epoxidation of 11-hydroxy-cucurbitadienol to produce 11-hydroxy-24,25 epoxy cucurbitadienol;
wherein the polypeptide comprises a polypeptide having at least 90% sequence identity to the amino acid sequence encoded by a nucleotide sequence set forth in any one of SEQ ID NOs:3-20, or 41 or the amino acid sequence set forth in SEQ ID NO:44;

(f) a gene encoding a polypeptide capable of catalyzing conversion of 11-hydroxy-24,25 epoxy cucurbitadienol to produce mogrol;
wherein the polypeptide comprises a polypeptide having at least 90% sequence identity to the amino acid sequence encoded by a nucleotide sequence set forth in SEQ ID NOs:38 or 40;

(g) a gene encoding a polypeptide capable of catalyzing epoxidation of cucurbitadienol to produce 24,25 epoxy cucurbitadienol;
wherein the polypeptide comprises a polypeptide having at least 90% sequence identity to the amino acid sequence encoded by a nucleotide sequence set forth in any one of SEQ ID NOs:3-20, or 41 or the amino acid sequence set forth in SEQ ID NO:44;

(h) a gene encoding a polypeptide capable of catalyzing hydroxylation of 24,25 epoxy cucurbitadienol to produce 11-hydroxy-24,25 epoxy cucurbitadienol;
wherein the polypeptide comprises a polypeptide having at least 90% sequence identity to the amino acid sequence encoded by a nucleotide sequence set forth in any one of SEQ ID NOs:3-20, or 41 or the amino acid sequence set forth in SEQ ID NO:44; and/or

(i) a one or more genes encoding a one or more polypeptides capable of glycosylation at C3′-OH, C24′-OH, C3′-OH and C24′-OH of the mogrol, at C3′-OH or C24′-OH of the glycosylated mogroside compound, or both C3′-OH, C24′-OH, C3′-OH and C24′-OH of the mogrol and C3′-OH or C24′-OH of the glycosylated mogroside compound or beta-1,6-glycosylation of the C2′ position of the 24-O-glucose, and/or beta-1,2-glycosylation of the C6′ position of the 3-O-glucose and/or the 24-O-glucose of the glycosylated mogroside compound;
wherein the one or more polypeptides comprises a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in any one of SEQ ID NOs:21-25, 48, 50, or 53 or a polypeptide having at least 90% sequence identity to the amino acid sequence encoded by the nucleotide sequence set forth in any one of SEQ ID NOs:26-36;

wherein at least one of the genes is a recombinant gene;
comprising growing the recombinant host cell in a culture medium, under conditions in which the genes are expressed; and
thereby producing the mogrol precursor, the mogroside precursor, and/or the mogroside compound in the recombinant host cell.

2. The method of claim 1 , wherein the recombinant host cell comprises genes recited in items (a), (c), (d), and (i).

3. The method of claim 1 , wherein the recombinant host cell comprises genes recited in items (a), (c), (e), (f), and (i).

4. The method of claim 1 , wherein the recombinant host cell comprises genes recited in items (a), (g), (h), (f), and (i).

5. The method of claim 1 , wherein the recombinant host cell comprises genes recited in items (c), (d), and (i).

6. The method of claim 1 , wherein the recombinant host cell comprises genes recited in items (b), (f), (h), and (i).

7. The method of claim 1 , wherein the recombinant host cell comprises genes recited in items (f), (h), and (i).

8. The method of claim 1 , wherein the recombinant host cell comprises genes recited in item (i).

9. The method of claim 1 , further comprising isolating the produced mogrol precursor, the mogroside precursor, and/or the mogroside compound.

10. The method of claim 1 , wherein the mogrol precursor is squalene, oxidosqualene, dioxidosqualene, cucurbitadienol, 24,25 epoxy cucurbitadienol, 11-hydroxy-cucurbitadienol, 11-hydroxy-24,25 epoxy cucurbitadienol, or 11-oxo-mogrol.

11. The method of claim 1 , wherein the mogroside precursor is mogrol or a glycosylated, a di-glycosylated, or a tri-glycosylated mogrol.

12. The method of claim 1 , wherein the mogroside compound is a mogroside compound glycosylated at C3′-OH, a mogroside compound glycosylated at C24′-OH, a mogroside compound glycosylated at C3′-OH and C24′-OH, a mogroside compound di-glycosylated at C24′ position, a mogroside compound tri-glycosylated at C24′ position, a mogroside compound glycosylated at C3′-OH and tri-glycosylated at C24′ position, a mogroside compound di-glycosylated at C3′-OH and tri-glycosylated at C24′ position, a mogroside compound di-glycosylated at C3′-OH and tri-glycosylated at C24′ position and oxidized at C11-OH, a mogroside compound di-glycosylated at C3′-OH and di-glycosylated at C24′ position, or an isomer thereof.

13. The method of claim 12 , wherein:
(a) the mogroside compound glycosylated at C3′-OH is mogroside I E1;
(b) the mogroside compound glycosylated at C24′-OH is mogroside I A1;
(c) the mogroside compound glycosylated at C3′-OH and C24′-OH is mogroside 11E;
(d) the mogroside compound di-glycosylated at C24′ position is mogroside IIA;
(e) the mogroside compound tri-glycosylated at C24′ position is mogroside IIIA1;
(f) the mogroside compound glycosylated at C3′-OH and tri-glycosylated at C24′ position is siamenoside 1;
(g) the mogroside compound di-glycosylated at C3′-OH and tri-glycosylated at C24′ position is mogroside V;
(h) the mogroside compound di-glycosylated at C3′-OH and tri-glycosylated at C24′ position and oxidized at C11-OH is 11-oxo-mogroside V; and
(i) the mogroside compound di-glycosylated at C3′-OH and di-glycosylated at C24′ position is mogroside IV.

14. The method of claim 1 , wherein the recombinant host cell is a microorganism that is a plant cell, a mammalian cell, an insect cell, a fungal cell, an algal cell, or a bacterial cell.

15. An in vivo method for transferring a sugar moiety to a mogrol, a glycosylated mogroside compound, or both the mogrol and the glycosylated mogroside compound, comprising contacting the mogrol, the glycosylated mogroside compound, or both the mogrol and the glycosylated mogroside compound with one or more recombinant polypeptides capable of glycosylation at C3′-OH, C24′-OH, C3′-OH and C24′-OH of the mogrol, at C3′-OH or C24′-OH of the glycosylated mogroside compound, or both C3′-OH, C24′-OH, C3′-OH and C24′-OH o

Assignees

• Evolva Sa

Inventors

• Liu Yaoquan
• Hansen Jorgen
• Houghton-Larsen Jens
• Murali Muthuswamy Panchapagesa
• Kumar Sathish
• Rasmussen Nina Nicoline

Classifications

• C12N9/0071

  acting on paired donors with incorporation of molecular oxygen (1.14) · CPC title

• C12Y303/00

  Hydrolases acting on ether bonds (3.3) · CPC title

• C12P33/20 Primary

  containing heterocyclic rings · CPC title

• C12Y114/13132

  Squalene monooxygenase (1.14.13.132) · CPC title

• C12P33/00

  Preparation of steroids · CPC title