Processing biomass
US-2015353974-A1 · Dec 10, 2015 · US
Microorganism producing O-acetyl homoserine and the method of producing O-acetyl homoserine using the same
US10465217B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10465217-B2 |
| Application number | US-201514901532-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 22, 2015 |
| Priority date | Jun 23, 2014 |
| Publication date | Nov 5, 2019 |
| Grant date | Nov 5, 2019 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
Disclosed is a microorganism of Escherichia sp. producing O-acetyl homoserine, and a method of producing O-acetyl homoserine in high yield using the microorganism.
First claim
Opening claim text (preview).
The invention claimed is: 1. A microorganism of Escherichia sp. producing O-acetyl homoserine, wherein the endogenous activity of citrate synthase is attenuated or inactivated, and a homoserine O-acetyltransferase is further introduced or enhanced, or an endogenous homoserine O-succinyltransferase is further modified to a polypeptide having 95% identity or above with SEQ ID NO: 16 and further having mutations at the G111E position and L112T or L112H positions to have the activity of homoserine O-acetyltransferase, wherein the microorganism has an improved O-acetyl homoserine production capability compared to a corresponding unmodified Escherichia sp. microorganism. 2. The microorganism of Escherichia sp. according to claim 1 , wherein the microorganism with the attenuated endogenous activity of citrate synthase has an amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 2. 3. The microorganism of Escherichia sp. according to claim 1 , wherein the activity of cystathionine gamma synthase, homoserine kinase, or both is further attenuated or inactivated compared to their endogenous activities. 4. The microorganism of Escherichia sp. according to claim 1 , wherein the activity of at least one protein selected from the group consisting of phosphoenolpyruvate carboxylase, aspartate aminotransferase, and aspartate semialdehyde dehydrogenase is further introduced or enhanced. 5. The microorganism of Escherichia sp. according to claim 1 , wherein the microorganism is Escherichia coli. 6. A method for producing O-acetyl homoserine, comprising: (a) culturing the microorganism of any of claims 1 to 3 , 4 and 5 ; and (b) recovering O-acetyl homoserine produced during the cultivation of the microorganism.
Assignees
Inventors
Classifications
Genes encoding for enzymes or proenzymes · CPC title
- C12P13/06Primary
Alanine; Leucine; Isoleucine; Serine; Homoserine · CPC title
Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases · CPC title
Citrate (Si)-synthase (2.3.3.1) · CPC title
Lyases (4.) · CPC title
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US10465217B2 cover?
- Disclosed is a microorganism of Escherichia sp. producing O-acetyl homoserine, and a method of producing O-acetyl homoserine in high yield using the microorganism.
- Who is the assignee on this patent?
- Cj Cheiljedang Corp
- What technology area does this patent fall under?
- Primary CPC classification C12P13/06. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Nov 05 2019 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).