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Polypeptides having peroxygenase activity and polynucleotides encoding same
US10465173B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10465173-B2 |
| Application number | US-201615290136-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 11, 2016 |
| Priority date | Aug 10, 2011 |
| Publication date | Nov 5, 2019 |
| Grant date | Nov 5, 2019 |
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Abstract
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The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
First claim
Opening claim text (preview).
The invention claimed is: 1. A nucleic acid construct comprising a polynucleotide encoding a polypeptide having peroxygenase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct production of the polypeptide in an expression host, and wherein the polypeptide has at least 90% sequence identity to amino acids 1 to 246 of SEQ ID NO: 2. 2. The nucleic acid construct of claim 1 , wherein the polypeptide has at least 95% sequence identity to amino acids 1 to 246 of SEQ ID NO: 2. 3. The nucleic acid construct of claim 1 , wherein the polypeptide has at least 97% sequence identity to amino acids 1 to 246 of SEQ ID NO: 2. 4. The nucleic acid construct of claim 1 , wherein the polypeptide has at least 98% sequence identity to amino acids 1 to 246 of SEQ ID NO: 2. 5. The nucleic acid construct of claim 1 , wherein the polypeptide has at least 99% sequence identity to amino acids 1 to 246 of SEQ ID NO: 2. 6. The nucleic acid construct of claim 1 , wherein the polypeptide consists of amino acids 1 to 246 of SEQ ID NO: 2. 7. The nucleic acid construct of claim 1 , wherein the polypeptide comprises an amino acid sequence including the motif: E-H-D-[G,A]-S-[L,I]-S-R, or R-[G,A]-P—C-P-X-[M,L]-N-[S,T]-L. 8. The nucleic acid construct of claim 1 , wherein the polypeptide comprises amino acids 1 to 246 of SEQ ID NO: 2. 9. The nucleic acid construct of claim 1 , wherein the polypeptide is a variant comprising a substitution, deletion, and/or insertion at one or more positions of amino acids 1 to 246 of SEQ ID NO: 2. 10. A recombinant host cell comprising the nucleic acid construct of claim 1 . 11. The recombinant host cell of claim 10 , wherein the polypeptide has at least 95% sequence identity to amino acids 1 to 246 of SEQ ID NO: 2. 12. The recombinant host cell of claim 10 , wherein the polypeptide has at least 97% sequence identity to amino acids 1 to 246 of SEQ ID NO: 2. 13. The recombinant host cell of claim 10 , wherein the polypeptide has at least 99% sequence identity to amino acids 1 to 246 of SEQ ID NO: 2. 14. The recombinant host cell of claim 10 , wherein the polypeptide consists of amino acids 1 to 246 of SEQ ID NO: 2. 15. The recombinant host cell of claim 10 , wherein the polypeptide comprises amino acids 1 to 246 of SEQ ID NO: 2. 16. A recombinant host cell transformed with a nucleic acid construct or expression vector comprising a polynucleotide encoding a polypeptide having peroxygenase activity, wherein the polypeptide having peroxygenase activity comprises an amino acid sequence having at least 90% sequence identity to amino acids 1 to 246 of SEQ ID NO: 2. 17. The recombinant host cell of claim 16 , wherein the polypeptide has at least 95% sequence identity to amino acids 1 to 246 of SEQ ID NO: 2. 18. The recombinant host cell of claim 16 , wherein the polypeptide has at least 97% sequence identity to amino acids 1 to 246 of SEQ ID NO: 2. 19. The recombinant host cell of claim 16 , wherein the polypeptide consists of amino acids 1 to 246 of SEQ ID NO: 2. 20. The recombinant host cell of claim 16 , wherein the polypeptide comprises amino acids 1 to 246 of SEQ ID NO: 2.
Assignees
Inventors
Classifications
Unspecific peroxygenase (1.11.2.1) · CPC title
containing oxidase or reductase · CPC title
using catalysts, e.g. selective catalysts · CPC title
aromatic · CPC title
polyhydric · CPC title
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- What does patent US10465173B2 cover?
- The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
- Who is the assignee on this patent?
- Novozymes As
- What technology area does this patent fall under?
- Primary CPC classification C12N9/0065. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Nov 05 2019 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).