Cell culture media

Patent metadata

Field	Value
Publication number	US-10465166-B2
Application number	US-201715687150-A
Country	US
Kind code	B2
Filing date	Aug 25, 2017
Priority date	Sep 24, 2013
Publication date	Nov 5, 2019
Grant date	Nov 5, 2019

Abstract

A cell culture medium comprising adenosine triphosphate; a carrier protein; cholesterol, linoleic acid, and lipoic acid; glutathione; at least one nucleotide salvage pathway precursor base; phosphoethanolamine; selenium; transferrin; triiodothyronine; all-trans-retinoic acid (ATRA) and vitamin C; zinc, magnesium, and copper; an agent that increases intracellular cAMP; epidermal growth factor (EGF); hydrocortisone; insulin; and charcoal stripped fetal bovine serum, wherein said cell culture medium is substantially free, if not entirely free, of vitamin D, androgenic hormones, androgenic ligands, estrogenic hormones, estrogenic ligands, and/or androgenic receptors.

First claim

What is claimed is:

1. A cell culture medium comprising:
(a) adenosine triphosphate; (b) a carrier protein; (c) cholesterol, linoleic acid, and lipoic acid; (d) glutathione; (e) at least one nucleotide salvage pathway precursor base; (f) phosphoethanolamine; (g) selenium; (h) transferrin; (i) triiodothyronine; (j) all-trans-retinoic acid (ATRA) and vitamin C; (k) zinc, magnesium, and copper; (l) an agent that increases intracellular cAMP; (m) epidermal growth factor (EGF); (n) hydrocortisone; (o) insulin; and (p) fetal bovine serum; and
wherein the culture medium further includes
(i) at least one androgen receptor (AR) inhibitor; or
(ii) at least one vitamin D receptor (VDR) inhibitor; or
(iii) at least one AR inhibitor and at least one VDR inhibitor.

2. The culture medium of claim 1 , wherein the culture medium includes at least one androgen receptor (AR) inhibitor.

3. The culture medium of claim 1 , wherein the culture medium includes at least one vitamin D receptor (VDR) inhibitor.

4. The culture medium of claim 1 , wherein the culture medium includes at least one androgen receptor (AR) inhibitor and at least one vitamin D receptor (VDR) inhibitor.

5. A method of culturing breast cancer cells comprising,
obtaining a sample of breast cancer cells from cancerous breast tissue,
adding an amount of the cell culture medium of claim 1 to the sample of breast cancer cells, and
maintaining the sample of breast cancer cells in the cell culture medium of claim 1 at conditions appropriate for cell growth.

6. A method of culturing breast cancer cells comprising,
obtaining a sample of breast cancer cells from cancerous breast tissue,
adding an amount of the cell culture medium of claim 2 to the sample of breast cancer cells, and
maintaining the sample of breast cancer cells in the cell culture medium of claim 2 at conditions appropriate for cell growth.

7. A method of culturing breast cancer cells comprising,
obtaining a sample of breast cancer cells from cancerous breast tissue,
adding an amount of the cell culture medium of claim 3 to the sample of breast cancer cells, and
maintaining the sample of breast cancer cells in the cell culture medium of claim 3 at conditions appropriate for cell growth.

8. A method of culturing breast cancer cells comprising,
obtaining a sample of breast cancer cells from cancerous breast tissue,
adding an amount of the cell culture medium of claim 4 to the sample of breast cancer cells, and
maintaining the sample of breast cancer cells in the cell culture medium of claim 4 at conditions appropriate for cell growth.

Assignees

• Univ Miami

Inventors

• Ince Tan

Classifications

• C12N5/0631 Primary

  Mammary cells · CPC title

• C12N2501/392

  Sexual steroids · CPC title

• C12N2501/39

  Steroid hormones · CPC title

• C12N2500/38

  Vitamins · CPC title

• C12N5/0693

  Tumour cells; Cancer cells · CPC title