Method for producing dry microbial cell powder

Patent metadata

Field	Value
Publication number	US-10465160-B2
Application number	US-201214111657-A
Country	US
Kind code	B2
Filing date	Apr 12, 2012
Priority date	Apr 14, 2011
Publication date	Nov 5, 2019
Grant date	Nov 5, 2019

Abstract

An object of the present invention is to develop a means capable of maintaining the enzymatic activity of a microorganism even in a dry state. Provided is a method for producing a dry microbial cell powder maintaining an enzyme titer, characterized by comprising adding a carbohydrate to a liquid of microbial cells having an enzymatic activity and then drying the liquid. Also provided is a dry microbial cell powder obtained by the method.

First claim

The invention claimed is:

1. A method for producing a dry microbial cell powder maintaining an enzyme titer, the method comprising:
adding a carbohydrate to a suspension of microbial cells having an enzymatic activity;
and then drying the suspension,
wherein the enzymatic activity is β-galactosidase activity,
wherein the carbohydrate is at least one selected from the group consisting of lactose and maltose,
wherein the carbohydrate is added in an amount of from 2 to 15 mass/vol % with respect to the amount of the suspension of the microbial cells,
wherein the microbial cell is a microorganism from a genus selected from the group consisting of genus Sporobolomyces , genus Rhodotorula , and genus Sirobasidium,
wherein the drying is spray drying, and
wherein the enzyme titer after the drying is 70% or higher than the enzyme titer before the drying.

2. The method according to claim 1 , wherein the carbohydrate is lactose.

3. A dry microbial cell powder, comprising microbial cells having an enzymatic activity and a carbohydrate,
wherein the cell powder is obtained by a method comprising:
adding a carbohydrate to a suspension of microbial cells having an enzymatic activity; wherein the carbohydrate is lactose and an amount of the lactose is at least 5 mass/vol % with respect to the amount of the suspension of the microbial cells, and
then drying the suspension by spray drying,
wherein 80% or higher of an enzyme titer immediately after drying is maintained after storage at room temperature for 1 year,
wherein the enzymatic activity is a β-galactosidase activity, and
the microbial cells are Sporobolomyces singularis cells.

Assignees

• Ikeda Masakazu
• Ishikawa Hiroshi
• Shimura Kisaku
• Fukuda Yuki
• Ito Masahiko
• Yakult Honsha Kk

Inventors

• Ikeda Masakazu
• Ishikawa Hiroshi
• Shimura Kisaku
• Fukuda Yuki
• Ito Masahiko

Classifications

• C12N1/04

  Preserving or maintaining viable microorganisms (immobilised microorganisms C12N11/00) · CPC title

• C12N1/16 Primary

  Yeasts; Culture media therefor · CPC title

• C12N9/2471

  Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase · CPC title

• C12N9/96

  Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates · CPC title

• C12N9/2468

  acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81) · CPC title