Fungal resistant plants expressing HCP7

Patent metadata

Field	Value
Publication number	US-10462994-B2
Application number	US-201414763652-A
Country	US
Kind code	B2
Filing date	Jan 10, 2014
Priority date	Jan 29, 2013
Publication date	Nov 5, 2019
Grant date	Nov 5, 2019

Abstract

The present invention relates to a method of increasing resistance against fungal pathogens of the family Phacosporaceae in plants and/or plant cells. This is achieved by increasing the expression of an HCP7 protein or fragment thereof in a plant, plant part and/or plant cell in comparison to wild type plants, wild type plant parts and/or wild type plant cells. Furthermore, the invention relates to transgenic plants, plant parts, and/or plant cells having an increased resistance against fungal pathogens, in particular, pathogens of the family Phacopsoraceae, and to recombinant expression vectors comprising a sequence that is identical or homologous to a sequence encoding an HCP7 protein.

First claim

The invention claimed is:

1. A method for increasing soybean rust resistance in a soybean plant, a soybean plant part, or a soybean plant cell, said method comprising
increasing the expression and/or activity of a HCP7 protein in the soybean plant, soybean plant part, or soybean plant cell in comparison to a wild type soybean plant, wild type soybean plant part or wild type soybean plant cell, wherein the HCP7 protein is encoded by an exogenous nucleic acid encoding a protein having an amino acid sequence with at least 80% identity to SEQ ID NO: 2, wherein the HCP7 protein confers increased resistance against soybean rust to the soybean plant, soybean plant part, or soybean plant cell in comparison to a wild type soybean plant, wild type soybean plant part or wild type soybean plant cell.

2. The method of claim 1 , comprising
(a) stably transforming a soybean plant cell with an expression cassette comprising an exogenous nucleic acid encoding an HCP7 protein having an amino acid sequence with at least 80% identity to SEQ ID NO: 2 in functional linkage with a promoter; and
(b) regenerating the soybean plant from the soybean plant cell.

3. A recombinant vector construct comprising:
(a) a nucleic acid encoding a protein having an amino acid sequence with at least 80% identity to SEQ ID NO: 2 operably linked with
(b) a heterologous promoter and
(c) a transcription termination sequence,
wherein expression of said recombinant vector construct in a soybean plant, soybean plant part, or soybean plant cell confers increased resistance against soybean rust thereto in comparison to a wild type soybean plant, wild type soybean plant part, or wild type soybean plant cell.

4. The method of claim 2 , wherein the promoter is a constitutive promoter, pathogen-inducible promoter, a mesophyll-specific promoter or an epidermis specific-promoter.

5. A transgenic soybean plant, transgenic soybean plant part, or transgenic soybean plant cell comprising an exogenous nucleic acid encoding an HCP7 protein comprising an amino acid sequence with at least 80% identity with SEQ ID NO: 2, wherein expression of said exogenous nucleic acid in the transgenic soybean plant, transgenic soybean plant part, or transgenic soybean plant cell confers increased resistance against soybean rust thereto in comparison to a wild type soybean plant, wild type soybean plant part, or wild type soybean plant cell.

6. A method for the production of a transgenic soybean plant, transgenic soybean plant part, or transgenic soybean plant cell having increased resistance against soybean rust, comprising
(a) introducing the recombinant vector construct of claim 3 into a soybean plant, a soybean plant part, or a soybean plant cell; and
(b) generating a transgenic soybean plant, transgenic soybean plant part, or transgenic soybean plant cell from the soybean plant, soybean plant part or soybean plant cell.

7. The method of claim 6 , further comprising the step of harvesting the seeds of the transgenic soybean plant and planting the seeds and growing the seeds to soybean plants, wherein the grown soybean plants comprise the exogenous nucleic acid encoding a protein having an amino acid sequence with at least 80% identity with SEQ ID NO: 2.

8. A harvestable part of the transgenic soybean plant of claim 5 , wherein the part comprises the exogenous nucleic acid encoding a protein having an amino acid sequence with at least 80% identity with SEQ ID NO: 2.

9. A product derived from the soybean plant of claim 5 , wherein the product comprises the exogenous nucleic acid encoding a protein having an amino acid sequence with at least 80% identity with SEQ ID NO: 2.

10. A method for the production of a product comprising
a) growing the soybean plant of claim 5 ; and
b) producing said product from or by the soybean plant or a part of the soybean plant.

11. The method of claim 10 comprising
a) growing the soybean plant and removing the harvestable parts from the soybean plant; and
b) producing said product from or by the harvestable parts of the soybean plant.

12. The method of claim 10 , wherein the product is meal or oil.

13. The method of claim 1 , wherein the resistance against soybean rust is resistance against Phakopsora meibomiae, Phakopsora pachyrhizi , or a combination thereof.

14. A method for breeding a fungal resistant soybean plant comprising
(a) crossing the soybean plant of claim 5 with a second soybean plant;
(b) obtaining seed from the cross of step (a);
(c) planting said seeds and growing the seeds to soybean plants; and
(d) selecting from the soybean plants produced in step (c) plants expressing the HCP7 protein.

15. A method for controlling soybean rust in a soybean plant, said method comprising providing a transgenic soybean plant comprising an exogenous nucleic acid encoding an HCP7 protein having an amino acid sequence with at least 80% identity to SEQ ID NO: 2, wherein expression of the nucleic acid leads to increased soybean rust resistance in said soybean plant as compared to a wild type soybean plant such that soybean rust is controlled in the transgenic soybean plant.

16. The method of claim 15 , wherein the soybean rust that is controlled is Phakopsora meibomiae and/or Phakopsora pachyrhizi.

17. The method of claim 1 , wherein the HCP7 protein has an amino acid sequence with at least 85% identity to SEQ ID NO:2.

18. The method of claim 1 , wherein the HCP7 protein has an amino acid sequence with at least 90% identity to SEQ ID NO:2.

19. The recombinant vector construct of claim 3 , wherein the HCP7 protein has an amino acid sequence with at least 85% identity to SEQ ID NO:2.

20. The recombinant vector construct of claim 3 , wherein the HCP7 protein has an amino acid sequence with at least 90% identity to SEQ ID NO:2.

21. The transgenic soybean plant, transgenic soybean plant part, or transgenic soybean plant cell of claim 5 , wherein the HCP7 protein has an amino acid sequence with at least 85% identity to SEQ ID NO:2.

22. The transgenic soybean plant, transgenic soybean plant part, or transgenic soybean plant cell of claim 5 , wherein the HCP7 protein has an amino acid sequence with at least 90% identity to SEQ ID NO:2.

Assignees

• Basf Plant Science Co Gmbh

Inventors

• Schultheiss Holger
• Flachmann Ralf

Classifications

• C12N15/8282

  for fungal resistance · CPC title

• C07K14/415

  from plants · CPC title

• C12N15/8279

  for biotic stress resistance, pathogen resistance, disease resistance · CPC title

• A01H1/02

  Methods or apparatus for hybridisation; Artificial pollination {; Fertility} · CPC title

• A01H5/10 Primary

  Seeds · CPC title