Method for searching candidate material for anti-cancer drug using localized surface plasmon resonance

Patent metadata

Field	Value
Publication number	US-10458911-B2
Application number	US-201615141196-A
Country	US
Kind code	B2
Filing date	Apr 28, 2016
Priority date	Apr 28, 2015
Publication date	Oct 29, 2019
Grant date	Oct 29, 2019

Abstract

A method for screening an anticancer candidate is disclosed. The method includes immobilizing gold nanoparticles onto a substrate; binding a protein involved in carcinogenesis and metastasis to the immobilized gold nanoparticles, recording a spectrum of the protein conjugate, and analyzing the spectrum to obtain reference data; adding a candidate inhibiting the activity of the protein to the protein conjugate, recording a spectrum of the mixture, and analyzing the spectrum to obtain comparative data; and comparing the reference data with the comparative data to determine whether the candidate inhibits the activity of the protein. The method enables screening of a candidate inhibiting the activity of a protein involved in carcinogenesis and metastasis in an accurate and convenient manner.

First claim

What is claimed is:

1. A method for screening an anticancer candidate, comprising:
immobilizing gold nanoparticles onto a substrate;
forming a STAT3 protein conjugate by binding a STAT3 protein involved in carcinogenesis and metastasis to the immobilized gold nanoparticles, recording a spectrum of the protein conjugate as the STAT3 protein is undergoing a phosphorylation or a dimerization step without the presence of the candidate, and analyzing the spectrum of the STAT3 protein conjugate to obtain reference data;
forming a mixture by adding a candidate inhibiting the activity of the STAT3 protein to the STAT3 protein conjugate, recording a spectrum of the mixture as the STAT3 protein is undergoing a phosphorylation or a dimerization, and analyzing the spectrum of the mixture to obtain comparative data; and
comparing the reference data with the comparative data to determine whether the candidate inhibits the activity of the protein,
wherein the addition of the candidate inhibiting the activity of the protein induces changes in the phosphorylation and dimerization of the STAT3 protein and the binding profile of the STAT3 protein with the gold nanoparticles on the substrate.

2. The method according to claim 1 , wherein the protein involved in carcinogenesis and metastasis is selected from proteins involved in immune signaling.

3. The method according to claim 1 , wherein the spectra are analyzed by measuring Rayleigh light scattering spectra of the gold nanoparticles using dark-field microspectroscopy.

4. The method according to claim 1 , wherein the spectra are analyzed by measuring localized surface plasmon resonance (LSPR) Δλ max shifts.

5. The method according to claim 1 , wherein the gold nanoparticles are amino-modified spherical gold nanoparticles.

Assignees

• Univ Korea Res & Bus Found

Inventors

• Sim Sang Jun
• Song So Jin

Classifications

• G01N21/554

  detecting the surface plasmon resonance of nanostructured metals, e.g. localised surface plasmon resonance · CPC title

• G01N21/47 Primary

  Scattering, i.e. diffuse reflection (G01N21/25, G01N21/41 take precedence {G01N21/55 takes precedence}) · CPC title

• G01N33/54346

  Nanoparticles · CPC title

• G01N33/553

  Metal or metal coated · CPC title

• G01N2333/47

  Assays involving proteins of known structure or function as defined in the subgroups · CPC title