SCN plants and methods for making the same

Patent metadata

Field	Value
Publication number	US-10457956-B2
Application number	US-201515540580-A
Country	US
Kind code	B2
Filing date	Dec 30, 2015
Priority date	Dec 31, 2014
Publication date	Oct 29, 2019
Grant date	Oct 29, 2019

Abstract

The invention relates to genes which may be utilized for resistance to soybean cyst nematode. More specifically the present disclosure relates to identification of gene(s) that can confer upon a soybean plant resistance to soybean cyst nematode (SCN) and methods to use these loci and genes to obtain soybean strains that are resistant to SCN.

First claim

What is claimed is:

1. An elite soybean plant, or a part thereof, comprising an introgression or genetic modification resulting in the elite soybean plant or a part thereof an increased expression of: an miRNA selected from SEQ ID NOs: 12, 25 and 34 and/or the gene of SEQ ID NO: 196, wherein compared to the host soybean plant or a part thereof used to produce the elite soybean plant or a part thereof, the elite soybean plant or a part thereof exhibits an increased resistance to an SCN infection.

2. The elite soybean plant, or a part thereof, of claim 1 , wherein said elite soybean plant or a part thereof exhibits an increased resistance to a Heterodera glycines infection.

3. The elite soybean plant, or a part thereof, of claim 1 , wherein said part is selected from the group consisting of a seed, endosperm, an ovule, and a pollen.

4. The elite soybean plant, or a part thereof, of claim 1 , wherein said elite soybean plant is transgenic.

5. The elite soybean plant, or part thereof, of claim 1 , wherein said elite soybean plant comprises:
i) one or more heterologous nucleic acid sequences encoding one or more miRNAs selected from SEQ ID NOs: 12, 25 and 34, or
ii) one or more native nucleic acid sequences encoding one or more miRNAs selected from SEQ ID NOs: 12, 25 and 34 operably linked to one or more heterologous promoters.

6. The elite soybean plant, or part thereof, of claim 1 , wherein said elite soybean plant comprises:
i) a heterologous nucleic acid sequence encoding the gene of SEQ ID NO: 196, or
ii) a native nucleic acid sequence encoding the gene of SEQ ID NO: 196 operably linked to a heterologous promoter.

7. The elite soybean plant, or part thereof, of claim 1 , wherein said elite soybean plant comprises:
i) one or more heterologous nucleic acid sequences encoding one or more miRNAs selected from SEQ ID NOs: 12, 25 and 34 and a heterologous nucleic acid sequence encoding the gene of SEQ ID NO: 196; or
ii) one or more heterologous promoters operably linked to one or more native nucleic acid sequences encoding one or more miRNAs selected from SEQ ID NOs: 12, 25 and 34 and a heterologous promoter operably linked to a native nucleic acid sequence encoding the gene of SEQ ID NO: 196.

8. A method for producing an elite soybean plant, or a part thereof comprising introgressing or genetically modifying a host soybean plant resulting in the elite soybean plant or a part thereof an increased expression of: an miRNA selected from SEQ ID NOs: 12, 25 and 34 and/or the gene of SEQ ID NO: 196, wherein compared to the host soybean plant or a part thereof used to produce the elite soybean plant or a part thereof, the elite soybean plant or a part thereof exhibits an increased resistance to an SCN infection.

9. The method of claim 8 , said method produces an SCN resistant elite soybean plant.

10. The method of claim 8 , wherein said method further comprises introducing into said soybean plant one or more traits selected from the group consisting of herbicide tolerance, increased yield, insect control, fungal disease resistance, virus resistance, nematode resistance, bacterial disease resistance, mycoplasma disease resistance, modified oils production, high oil production, high protein production, germination and seedling growth control, enhanced animal and human nutrition, lower raffinose, environmental stress resistance, increased digestibility, improved processing traits, improved flavor, improved nitrogen fixation, improved hybrid seed production, reduced allergenicity, and improved production of biofuels.

11. The method of claim 10 , wherein said soybean plant produced by said method is resistant to a herbicide selected from the group consisting of glyphosate, dicamba, glufosinate, sulfonylurea, bromoxynil, 2,4-Dichlorophenoxyacetic acid, and norflurazon.

12. The method of claim 8 , wherein said soybean plant produced by said method is transgenic.

13. The elite soybean plant, or part thereof, of claim 1 , wherein said elite soybean plant comprises:
i) a heterologous nucleic acid sequence encoding the miRNA of SEQ ID NO: 12, or
ii) a native nucleic acid sequence encoding the miRNA of SEQ ID NO: 12 operably linked to a heterologous promoter.

14. The elite soybean plant, or part thereof, of claim 1 , wherein said elite soybean plant comprises:
i) a heterologous nucleic acid sequence encoding the miRNA of SEQ ID NO: 25, or
ii) a native nucleic acid sequence encoding the miRNA of SEQ ID NO: 25 operably linked to a heterologous promoter.

15. The elite soybean plant, or part thereof, of claim 1 , wherein said elite soybean plant comprises:
i) a heterologous nucleic acid sequence encoding the miRNA of SEQ ID NO: 34, or
ii) a native nucleic acid sequence encoding the miRNA of SEQ ID NO: 34 operably linked to a heterologous promoter.

16. The elite soybean plant, or part thereof, of claim 1 , wherein said elite soybean plant comprises:
i) a heterologous nucleic acid sequence encoding the gene of SEQ ID NO: 196, or
ii) a native nucleic acid sequence encoding the gene of SEQ ID NO: 196 operably linked to a heterologous promoter.

Assignees

• Univ Tennessee Res Found

Inventors

• Hewezi Tarek Abdelfattah
• Rambani Aditi
• Stewart Jr C Neal
• Mazarei Mitra
• Pantalone Vincent

Classifications

• C12N15/8285 Primary

  for nematode resistance · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12Q2600/13

  Plant traits · CPC title

• C12Q2600/178

  miRNA, siRNA or ncRNA · CPC title