Compositions and methods of plant breeding using high density marker information

Patent metadata

Field	Value
Publication number	US-10455783-B2
Application number	US-201213440713-A
Country	US
Kind code	B2
Filing date	Apr 5, 2012
Priority date	Aug 15, 2006
Publication date	Oct 29, 2019
Grant date	Oct 29, 2019

Abstract

The present invention relates to breeding methods to enhance the germplasm of a plant. The methods describe the identification and accumulation of preferred haplotype genomic regions in the germplasm of breeding populations of maize (Zea mays) and soybean (Glycine max). The invention also relates to maize and soybean plants comprising preferred haplotypes.

First claim

What is claimed is:

1. A method for preparing a transgenic soybean plant, the method comprising the steps of:
transforming a parent plant comprising a chromosomal region at map position 114.30 cM to 115.90 cM on chromosome 6 identifiable by SEQ ID NOs:518 and 519, with a recombinant DNA and producing at least two transgenic progeny soybean plants; and
selecting a transgenic progeny soybean plant for breeding from said at least two transgenic progeny soybean plants, wherein said selected transgenic progeny soybean plant comprises said transgene comprising said recombinant DNA linked within 10 cM of said chromosomal region at map position 114.30 to 115.90 on chromosome 6 identifiable by SEQ ID NOs:518 and 519.

2. The method of claim 1 , wherein said transgene confers a phenotypic trait selected from the group consisting of herbicide tolerance, disease resistance, insect resistance, pest resistance, altered fatty acid, protein or carbohydrate metabolism, increased grain yield, increased oil, enhanced nutritional content, increased growth rates, enhanced stress tolerance, preferred maturity, enhanced organoleptic properties, altered morphological characteristics, and sterility.

3. The method of claim 1 , wherein said transgene comprises a recombinant DNA that is transcribed into a functional mRNA molecule that is translated and expressed as a protein product.

4. The method of claim 1 , wherein said transgene comprises a recombinant DNA that is transcribed into a double-stranded RNA for RNA interference (RNAi).

5. The method of claim 4 , wherein said double-stranded RNA is a small interfering RNA (siRNA).

6. The method of claim 4 , wherein said double-stranded RNA is a trans-acting small interfering RNA (ta-siRNA).

7. The method of claim 4 , wherein said double-stranded RNA is a microRNA (miRNA).

8. The method of claim 1 , further comprising crossing said selected transgenic soybean plant with a second soybean plant to produce a progeny plant having said transgene linked to said chromosomal region at map position 114.30 to 115.90 on chromosome 6 identifiable by SEQ ID NOs:518 and 519.

9. The method of claim 1 , wherein said transgene and said chromosomal region at map position 114.30 to 115.90 on chromosome 6 identifiable by SEQ ID NOs:518 and 519 are within 5 cM of each other.

10. The method of claim 9 , wherein said transgene and said chromosomal region at map position 114.30 to 115.90 on chromosome 6 identifiable by SEQ ID NOs:518 and 519 are within 1 cM of each other.

11. The method of claim 10 , wherein said transgene is within said chromosomal region at map position 114.30 to 115.90 on chromosome 6 identifiable by SEQ ID NOs:518 and 519.

12. The method of claim 2 , wherein said transgene confers herbicide tolerance.

Assignees

• Bull Jason
• Butruille David
• Eathington Sam
• Edwards Marlin
• Gupta Anju
• Johnson G Richard
• Reiter Robert Stefan
• Monsanto Technology Llc

Inventors

• Bull Jason
• Butruille David
• Eathington Sam
• Edwards Marlin
• Gupta Anju
• Johnson G Richard
• Reiter Robert Stefan

Classifications

• C12Q2600/172

  Haplotypes · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12Q2600/13

  Plant traits · CPC title

• A01H5/10

  Seeds · CPC title

• A01H1/04 Primary

  Processes of selection {involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection} · CPC title