Aryloxyphenoxypropionate tolerance in turfrgass species and use of AAD1 as a selectable marker

Patent metadata

Field	Value
Publication number	US-10450549-B2
Application number	US-201615287630-A
Country	US
Kind code	B2
Filing date	Oct 6, 2016
Priority date	Apr 30, 2004
Publication date	Oct 22, 2019
Grant date	Oct 22, 2019

Abstract

The present disclosure relates to the use of a grass-active herbicide postemergently applied to AAD1-transformed turfgrasses to selectively control grass weeds in a turf grass crop. Also described is the use of AAD1 as a selectable marker in the production of transgenic turfgrass.

First claim

What is claimed is:

1. A host cell of a grass species that contains a nucleic acid molecule comprising a polynucleotide that encodes a polypeptide having at least 95% sequence identity with the amino acid sequence of SEQ ID NO:9, wherein the polypeptide has (R)-phenoxypropionate/α-ketoglutarate-dioxygenase activity.

2. The host cell of claim 1 , wherein the polynucleotide is operably linked to a promoter.

3. The host cell of claim 2 , wherein the promoter is the AtUbi10 promoter.

4. A transgenic plant, plant part, plant organ, or plant seed that comprises the host cell of claim 1 .

5. The transgenic plant, plant part, plant organ, or plant seed of claim 4 , wherein the plant, plant part, plant organ, or plant seed is tolerant to glyphosate or glufosinate, when compared to a wild-type plant of the same species.

6. The host cell of claim 1 , wherein the polynucleotide encodes the polypeptide of SEQ ID NO:9, SEQ ID NO:10,SEQ ID NO:11.

7. A tissue culture of regenerable cells produced from the plant, plant part, plant organ, or plant seed of claim 4 .

8. A protoplast produced from the transgenic plant, plant part, plant organ, or plant seed of claim 4 .

9. The host cell of claim 1 , wherein the polynucleotide comprises a nucleotide sequence that is at least 60% identical to SEQ ID NO:3, SEQ ID NO:4, or SEQ ID NO:5.

10. A transgenic grass plant that comprises the host cell of claim 1 .

11. The transgenic grass plant of claim 10 , wherein the plant is resistant to an amount of an aryloxyphenoxypropionate herbicide that significantly damages a wild-type plant of the same species.

12. The transgenic grass plant of claim 11 , wherein the plant is also resistant to an amount of a phenoxy auxin herbicide that significantly damages a wild-type plant of the same species.

13. A method of generating a host plant cell of a grass species that is resistant to an aryloxyphenoxypropionate herbicide and/or a phenoxy auxin herbicide, the method comprising:
transforming the host plant cell with a nucleic acid molecule comprising a polynucleotide that encodes a polypeptide having at least 95% sequence identity with the amino acid sequence of SEQ ID NO:9, wherein the polypeptide has (R)-phenoxypropionate/α-ketoglutarate-dioxygenase activity.

14. A method for controlling weeds in an area under cultivation containing transgenic grass plants, the method comprising:
planting a grass plant or a grass plant seed comprising a nucleic acid molecule comprising a polynucleotide that encodes a polypeptide having at least 95% sequence identity with the amino acid sequence of SEQ ID NO:9, wherein the polypeptide has (R)-phenoxypropionate/α-ketoglutarate-dioxygenase activity; and
applying to the area under cultivation a sufficient amount of herbicide to control weeds in the area under cultivation without significantly affecting the grass plant or grass plant seed.

15. The method according to claim 14 , wherein the herbicide is an aryloxyphenoxypropionate herbicide and/or a phenoxy auxin herbicide.

16. The method according to claim 14 , wherein the herbicide is selected from the group consisting of 2, 4-D, quizalofop, and haloxyfop.

17. The method according to claim 14 , wherein the herbicide is a mixture of an aryloxyphenoxypropionate herbicide and a phenoxy auxin herbicide.

18. The host cell of claim 6 , wherein the polynucleotide comprises a nucleotide sequence that is at least 60% identical to SEQ ID NO:3, SEQ ID NO:4, or SEQ ID NO:5.

19. The host cell of claim 6 , wherein the polynucleotide comprises a nucleotide sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, and SEQ ID NO:5.

20. The host cell of claim 9 , wherein the polynucleotide comprises a nucleotide sequence that is at least 80% identical to SEQ ID NO:3, SEQ ID NO:4, or SEQ ID NO:5.

21. The host cell of claim 9 , wherein the polynucleotide comprises a nucleotide sequence that is at least 90% identical to SEQ ID NO:3, SEQ ID NO:4, or SEQ ID NO:5.

Assignees

• Dow Agrosciences Llc

Inventors

• Yerkes Carla N
• Zilinskas Barbara A
• Merlo Donald J
• Wright Terry R
• Lira Justin
• Arnold Nicole
• Thompson Gary D
• Zhou Ning
• Worden Andrew

Classifications

• C12N9/0069

  acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13) · CPC title

• C12N15/8209

  Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers · CPC title

• C12N15/8274 Primary

  for herbicide resistance · CPC title

• C12N9/0071 Primary

  acting on paired donors with incorporation of molecular oxygen (1.14) · CPC title