Antibody-binding polypeptide, antibody-binding fusion polypeptide, and adsorption material

Patent metadata

Field	Value
Publication number	US-10450344-B2
Application number	US-201715469660-A
Country	US
Kind code	B2
Filing date	Mar 27, 2017
Priority date	Sep 29, 2014
Publication date	Oct 22, 2019
Grant date	Oct 22, 2019

Abstract

Provided are an antibody-binding polypeptide as set forth in any one of SEQ ID NOS: 1 to 18 and an adsorption material of an antibody or antibody derivative in which the antibody-binding polypeptide is immobilized on a water-insoluble carrier. These antibody-binding polypeptide and adsorption material have excellent antibody binding properties and selectivity, and also excellent alkali resistance and temporal stability.

First claim

What is claimed is:

1. An antibody-binding polypeptide having a sequence homology of 85% or more to a polypeptide as set forth in any one of SEQ ID NOs: 6-9 and 15-18.

2. The antibody-binding polypeptide according to claim 1 , wherein 1 to 24 ethylene glycol units are covalently bound to at least one place selected from the N-terminal, the C-terminal, and the amino acid residue side chain of the antibody-binding polypeptide.

3. The antibody-binding polypeptide according to claim 1 , wherein at least one place selected from the N-terminal, the C-terminal, and the amino acid residue side chain of the antibody-binding polypeptide is modified.

4. An antibody-binding polypeptide having a sequence as set forth in any one of SEQ ID NOs: 6-9 and 15-18.

5. The antibody-binding polypeptide according to claim 4 , wherein 1 to 24 ethylene glycol units are covalently bound to at least one place selected from the N-terminal, or the C-terminal, of the antibody-binding polypeptide.

6. The antibody-binding polypeptide according to claim 4 , wherein at least one place selected from the N-terminal, or the C-terminal, of the antibody-binding polypeptide is modified.

7. An antibody-binding fusion polypeptide in which 2 to 10 domain units are fused by a covalent bond, taking an antibody-binding polypeptide having a sequence as set forth in any one of SEQ ID NOs: 6-9 and 15-18 as one domain unit,
wherein the antibody-binding fusion polypeptide further comprises a linker connecting between the foregoing domain units, and
wherein the linker is at least one linker selected from the group consisting of a PEG linker consisting of 1 to 24 ethylene glycol units per one linker.

8. The antibody-binding fusion polypeptide according to claim 7 , wherein 1 to 24 ethylene glycol units are covalently bound to at least one place selected from the N-terminal, or the C-terminal, of the antibody-binding fusion polypeptide.

9. The antibody-binding fusion polypeptide according to claim 7 , wherein at least one place selected from the N-terminal, or the C-terminal, of the antibody-binding fusion polypeptide is modified.

10. The antibody-binding fusion polypeptide according to claim 7 , wherein the total molecular weight of amino acid residues contained in the domain unit and amino acid residues contained in the linker is 5000 or less.

11. An adsorption material of an antibody or an antibody derivative, in which an antibody-binding polypeptide having a sequence homology of 85% or more to a polypeptide as set forth in any one of SEQ ID NOs: 6-9 and 15-18, an antibody-binding polypeptide as set forth in any one of SEQ ID NOs: 6-9 and 15-18, or an antibody-binding fusion polypeptide in which 2 to 10 domain units are fused by a covalent bond, taking an antibody-binding polypeptide as set forth in any one of SEQ ID NOs: 6-9 and 15-18 as one domain unit is immobilized on a water-insoluble carrier,
wherein the antibody-binding fusion polypeptide further comprises a linker connecting between the foregoing domain units, and
wherein the linker is at least one linker selected from the group consisting of a PEG linker consisting of 1 to 24 ethylene glycol units per one linker.

Assignees

• Fujifilm Corp

Inventors

• Minami Koichi

Classifications

• C07K1/22 Primary

  Affinity chromatography or related techniques based upon selective absorption processes · CPC title

• C07K14/001

  by chemical synthesis · CPC title

• C07K7/08

  having 12 to 20 amino acids (gastrins C07K14/595; somatostatins C07K14/655; melanotropins C07K14/68) · CPC title

• C07K14/00 Primary

  Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof · CPC title

• C07K17/00

  Carrier-bound or immobilised peptides (carrier-bound or immobilised enzymes C12N11/00); Preparation thereof · CPC title