Compositions and methods for cartilage defect repair using a RHAMM-mimetic peptide

Patent metadata

Field	Value
Publication number	US-10449229-B2
Application number	US-201515504938-A
Country	US
Kind code	B2
Filing date	Aug 19, 2015
Priority date	Aug 19, 2014
Publication date	Oct 22, 2019
Grant date	Oct 22, 2019

Abstract

Provided are methods and compositions for cartilage repair. The method involves performing a surgical procedure at the site of a cartilage defect and administering a composition comprising a receptor for hyaluronan mediated motility (RHAMM)-mimetic peptide, and a high molecular weight hyaluronan.

First claim

The invention claimed is:

1. A method for repairing a defect in a cartilage of a joint in a subject comprising performing a surgical procedure at a site of the defect in the cartilage, and administering: i.) a composition comprising a RHAMM-mimetic peptide and a high molecular weight hyaluronan (HMWHA) to the site of the defect in the cartilage, or
ii. a composition comprising a RHAMM-mimetic peptide and a composition comprising high molecular weight hyaluronan (HMWHA) to the site of the defect in the cartilage, wherein the RHAMM-mimetic peptide and the HMWHA are administered in a ratio of from 1:1 to 1:1000 by weight.

2. The method of claim 1 , wherein the RHAMM-mimetic peptide composition and the HMWHA composition of ii) are administered concurrently or sequentially.

3. The method of claim 1 , wherein the administration is carried out via intra-articular injection.

4. The method of claim 1 , wherein the surgical procedure is a microfracture.

5. The method of claim 1 , wherein the RHAMM-mimetic peptide comprises the sequence of SEQ ID NO: 1.

6. The method of claim 1 , wherein the HMWHA has an average molecular weight of at least 500 kDa.

7. The method of claim 1 , wherein the concentration of HMWHA is from 1 mg/ml to 40 mg/ml.

8. The method of claim 1 , wherein the concentration of the RHAMM-mimetic peptide is from 5 μg/ml to 3 mg/ml.

9. The method of claim 1 , further comprising repeating the administration of the composition or i) or ii) to the site of the cartilage defect.

10. The method of claim 1 , further comprising administering autologous mesenchymal stem cells to the site of the cartilage defect.

Assignees

• Univ New York
• London Health Sci Ct Res Inc

Inventors

• Cowman Mary K
• Kirsch Thorsten
• Strauss Eric J
• Turley Eva Ann
• Toelg Cornelia
• Luyt Leonard G

Classifications

• C07K14/70585

  CD44 · CPC title

• A61P19/02

  for joint disorders, e.g. arthritis, arthrosis · CPC title

• C07K14/70596

  Molecules with a "CD"-designation not provided for elsewhere · CPC title

• A61K38/10 Primary

  Peptides having 12 to 20 amino acids {(A61K38/043 - A61K38/046 take precedence)} · CPC title

• A61K31/728

  Hyaluronic acid · CPC title