Cell culture apparatus

Patent metadata

Field	Value
Publication number	US-10443028-B2
Application number	US-201515521811-A
Country	US
Kind code	B2
Filing date	Mar 20, 2015
Priority date	Mar 20, 2015
Publication date	Oct 15, 2019
Grant date	Oct 15, 2019

Abstract

Techniques for providing in vivo environments to in vitro cells by periodically applying mechanical stimuli to in vitro cells are provided for effective studies on cell culture. A first embodiment of the present invention provides a cell culture apparatus including: a first layer including an electroactive polymer material and elongated in at least one direction by external force applied thereto; a first fixing part fixing both ends of the first layer in the direction in which the first layer is elongated, so as to maintain the elongation of the first layer; a second layer including the electroactive polymer material and spaced apart from a surface of the first layer, the second layer being elongated in at least one direction by external force applied thereto; a second fixing part fixing both ends of the second layer in the direction in which the second layer is elongated, so as to maintain the elongation of the second layer; a first electrode part applied to a region of the first layer; and a second electrode part applied to a region of the second layer, wherein the second layer is provided in such a manner that a cell to be cultured is sandwiched between the surface of the first layer and a surface of the second layer.

First claim

The invention claimed is:

1. A cell culture apparatus comprising:
a first layer comprising an electroactive polymer material and elongated in at least one direction by external force applied thereto;
a first fixing part fixing both ends of the first layer in a first direction in which the first layer is elongated and maintaining an elongation orientation of the first layer;
a second layer comprising the electroactive polymer material and spaced apart from a surface of the first layer, the second layer being elongated in at least one direction by external force applied thereto;
a second fixing part fixing both ends of the second layer in a second direction in which the second layer is elongated and maintaining an elongation orientation of the second layer;
a first electrode part applied to a region of the first layer;
a second electrode part applied to a region of the second layer and,
a control module configured to adjust voltage applied to the first and second electrode parts to be different in at least one of amplitude, frequency, and phase, the first layer and the second layer having different deformation sizes and directions from one another,
wherein the first direction and the second direction are different from one another,
wherein a cell to be cultured is interposed between the surface of the first layer and a surface of the second layer, and
wherein at least one of elongation orientation, shape, and area of the first layer and the second layer are different from one another.

2. The cell culture apparatus of claim 1 , wherein when the voltage is applied to the first electrode part and the second electrode part, a size deformation ratio of the region of the first layer to which the first electrode part is applied is different from a size deformation ratio of the region of the second layer to which the second electrode part is applied.

3. The cell culture apparatus of claim 2 , wherein a number of layers stacked in the first layer is different from a number of layers stacked in the second layer, and when the voltage is applied to the first electrode part and the second electrode part, the size deformation ratio of the region of the first layer to which the first electrode part is applied is different from the size deformation ratio of the region of the second layer to which the second electrode part is applied.

4. The cell culture apparatus of claim 2 , wherein a number of layers which include the electroactive polymer material and are stacked in the first layer is different from a number of layers, which include the electroactive polymer material and are stacked in the second layer, and when the voltage is applied to the first electrode part and the second electrode part, the size deformation ratio of the region of the first layer to which the first electrode part is applied is different from the size deformation ratio of the region of the second layer to which the second electrode part is applied.

5. The cell culture apparatus of claim 1 , wherein at least one of sizes and patterns of the first electrode part and the second electrode part are different from one another.

6. The cell culture apparatus of claim 1 , wherein the first layer and the second layer have different thickness.

7. The cell culture apparatus of claim 1 , wherein the first layer and the second layer have different concave-convex patterns from one another.

8. The cell culture apparatus of claim 1 , wherein the control module has a configuration to have different shear deformation of the first layer and the second layer by adjusting the voltage in different phase.

9. A cell culture apparatus comprising:
a first layer comprising an electroactive polymer material and elongated in at least one direction by external force applied thereto;
a first fixing part fixing both ends of the first layer in a first direction in which the first layer is elongated and maintaining an elongation orientation of the first layer;
a second layer comprising the electroactive polymer material and spaced apart from a surface of the first layer, the second layer being elongated in at least one direction by external force applied thereto;
a second fixing part fixing both ends of the second layer in a second direction in which the second layer is elongated and maintaining an elongation orientation of the second layer;
a first electrode part applied to a region of the first layer; and
a second electrode part applied to a region of the second layer,
wherein the first direction and the second direction are different from one another,
wherein a cell to be cultured is interposed between the surface of the first layer and a surface of the second layer, and
wherein at least one of shape, and area of the first layer and the second layer are different from one another.

Assignees

• Univ Chung Ang Ind Acad Coop Found

Inventors

• Chang Seung Hwan
• Bae Ji Hun

Classifications

• C12M1/02

  with agitation means; with heat exchange means · CPC title

• C12M1/14

  with means providing thin layers or with multi-level trays · CPC title

• C12M35/04 Primary

  Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli · CPC title

• C12M1/42

  Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves · CPC title

• C12M3/04

  with means providing thin layers · CPC title