Artificial forisome body with SEO-F fusion proteins, plant or yeast cells comprising vectors which code for these proteins, as well as vectors which code for SEO-F fusion proteins

Patent metadata

Field	Value
Publication number	US-10442840-B2
Application number	US-201314399472-A
Country	US
Kind code	B2
Filing date	May 2, 2013
Priority date	May 9, 2012
Publication date	Oct 15, 2019
Grant date	Oct 15, 2019

Abstract

The invention relates to artificial forisome bodies including a fusion protein of at least one SEO-F protein or an at least 50-amino acid portion thereof, and at least one additional protein or peptide, with the exception of GFP and the Venus protein, wherein in an embodiment the forisome body further includes an unfused SEO-F protein or a form of the protein having C-terminal deletions of up to 45 amino acids and/or N-terminal deletions of up to 13 amino acids, wherein the unfused SEO-F protein is selected from proteins having the property of being capable of forming homomeric forisome bodies in the absence of additional SEO-F proteins.

First claim

What is claimed is:

1. An artificial forisome body comprising a fusion protein of a SEO-F (Sieve Element Occlusion by Forisome) protein having the amino acid sequence of SEQ ID NO: 1, or a fragment of the amino acid sequence of SEQ ID NO: 1, wherein said fragment results from deleting up to 45 amino acids from the C-terminus and/or up to 13 amino acids from the N-terminus of the amino acid sequence of SEQ ID NO: 1, and at least one additional protein, wherein the at least one additional protein is not a fluorescent protein, and is selected from the group consisting of glucose-6-phosphate dehydrogenase, hexokinase, phospho-glucoisomerase and antibodies, and
wherein the forisome body further comprises an unfused SEO-F protein, wherein said unfused SEO-F protein is
(i) a protein having the amino acid sequence of SEQ ID NO: 1, or (ii) a protein comprising a fragment of the amino acid sequence of SEQ ID NO: 1, wherein said fragment results from deleting up to 45 amino acids from the C-terminus and/or up to 13 amino acids from the N-terminus of the amino acid sequence of SEQ ID NO: 1,
and wherein the unfused SEO-F protein has the ability to form homomeric forisome bodies in the absence of additional SEO-F proteins.

Assignees

• Fraunhofer Ges Forschung
• Fraunhofer Ges Zur Foerderung Der Angewandten Forchung E V

Inventors

• Mueller Boje
• Pruefer Dirk
• Fischer Rainer

Classifications

• C12N15/8242

  with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits · CPC title

• C12Y503/01009

  Glucose-6-phosphate isomerase (5.3.1.9) · CPC title

• C12Y207/01001

  Hexokinase (2.7.1.1) · CPC title

• C12Y101/01049

  Glucose-6-phosphate dehydrogenase (1.1.1.49) · CPC title

• C12N9/92

  Glucose isomerase {(5.3.1.5; 5.3.1.9; 5.3.1.18)} · CPC title