Photocrosslinked biodegradable hydrogel
US-9642914-B2 · May 9, 2017 · US
Protein delivery from stem cell microcarriers
US10441610B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10441610-B2 |
| Application number | US-201113988929-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 23, 2011 |
| Priority date | Nov 23, 2010 |
| Publication date | Oct 15, 2019 |
| Grant date | Oct 15, 2019 |
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- Title
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Abstract
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Disclosed are methods and compositions of microbead carriers for delivery of cells and other biologically active substances to diseased or damaged tissue in a subject in need thereof.
First claim
Opening claim text (preview).
What is claimed is: 1. A composition for affecting chondrogenic gene expression when contacting a chondrocyte comprising injectable biodegradable polymeric hydrogel controlled-release microbeads, wherein the microbeads comprise mesenchymal stem cells and alginate, wherein alginate lyase is incorporated into the alginate, and wherein the expression, production, or secretion of an angiogenic factor or a hypertrophic factor or both by the mesenchymal stem cells is inhibited by contact with chondrogenic medium, or the expression, production, or secretion of a chondrogenic factor or an anti-hypertrophic factor or both by the mesenchymal stem cells is stimulated by contact with chondrogenic medium, and wherein the mesenchymal stem cells are obtainable by contact with a chondrogenic medium for 5 days. 2. The composition of claim 1 , wherein the microbeads are about 200 micrometers or less in diameter. 3. The composition of claim 1 , wherein the mesenchymal stem cells are adipose stem cells. 4. The composition of claim 3 , wherein the angiogenic factor is VEGF-A or FGF-2, or a combination of both. 5. The composition of claim 3 , wherein the hypertrophic factor is FGF-18. 6. The composition of claim 3 , wherein the chondrogenic factor is TGF-β1, TGF-β2, TGF-β3, IGF-1, or PTHrP, or a combination thereof. 7. The composition of claim 3 , wherein the anti-hypertrophic factor is Noggin. 8. The composition of claim 1 , wherein the microbeads are a renewable reservoir for the angiogenic factor, hypertrophic factor, chondrogenic factor, or anti-hypertrophic factor, or a combination thereof, which are expressed, produced, or secreted by the mesenchymal stem cells. 9. The composition of claim 8 , wherein the mesenchymal stem cells are adipose stem cells. 10. The composition of claim 1 , wherein the mesenchymal stem cells are are conditioned with chondrogenic medium before the stem cells are encapsulated in the microbeads. 11. The composition of claim 1 , wherein the mesenchymal stem cells are conditioned with chondrogenic medium after the stem cells are encapsulated in the microbeads.
Assignees
Inventors
Classifications
Amino acids · CPC title
Drugs for skeletal disorders · CPC title
Chondrocytes; Cartilage · CPC title
capsules, e.g. microcapsules · CPC title
Insulin-transferrin; Insulin-transferrin-selenium · CPC title
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- What does patent US10441610B2 cover?
- Disclosed are methods and compositions of microbead carriers for delivery of cells and other biologically active substances to diseased or damaged tissue in a subject in need thereof.
- Who is the assignee on this patent?
- Boyan Barbara Dale, Schwartz Zvi, Lee Christopher S D, and 3 more
- What technology area does this patent fall under?
- Primary CPC classification A61K35/28. Mapped technology areas include Human Necessities.
- When was this patent published?
- Publication date Tue Oct 15 2019 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).