Methods and compositions for plant pest control

Patent metadata

Field	Value
Publication number	US-10435701-B2
Application number	US-201715682205-A
Country	US
Kind code	B2
Filing date	Aug 21, 2017
Priority date	Mar 14, 2013
Publication date	Oct 8, 2019
Grant date	Oct 8, 2019

Abstract

Provided are methods and compositions to improve fungal disease resistance and/or nematode resistance in various crop plants. Also provided are combinations of compositions and methods to improve fungal disease resistance and/or nematode resistance in various crop plants.

First claim

What is claimed is:

1. A method for producing a soybean plant exhibiting an improvement in nematode disease resistance comprising topically applying to the soybean plant surface a composition that comprises:
a. at least one exogenous double stranded RNA (dsRNA) of 25 to about 95 nucleotides in length, wherein the dsRNA comprises a segment of 25 contiguous nucleotides that are identical or complementary to nucleic acid residues 1149 to 1806 of SEQ ID NO: 21, wherein said exogenous dsRNA is not operably linked to a promoter or to a viral vector and wherein the dsRNA is not transcribed from DNA integrated into a chromosome of the plant; and
b. a transfer agent that conditions the plant surface to permeation by the dsRNA into cells of the plant, wherein said soybean plant exhibits an improvement in nematode disease resistance that results from suppression of an endogenous soybean DND1 gene.

2. The method of claim 1 , wherein said transfer agent comprises a surfactant, lipid, or combination thereof.

3. The method of claim 1 , wherein said dsRNA is selected from the group consisting of SEQ ID NO: 38, 39, 43, and 45.

4. The method of claim 1 , wherein said composition comprises any combination of two or more of said dsRNA molecules.

5. The method of claim 1 , wherein said composition further comprises a non-polynucleotide herbicidal molecule, a polynucleotide herbicidal molecule, a polynucleotide that suppresses an herbicide target gene, an insecticide, a fungicide, a nematocide, or a combination thereof.

6. The method of claim 1 , wherein said transfer agent comprises an organosilicone preparation.

7. A soybean plant obtained by the method of claim 1 , said soybean plant comprising an exogenous dsRNA of 25 to about 95 nucleotides in length, wherein the dsRNA comprises a segment of 25 contiguous nucleotides that are identical or complementary to nucleic acid residues 1149 to 1806 of SEQ ID NO: 21, wherein said exogenous dsRNA is not operably linked to a promoter or to a viral vector, is not transcribed from DNA integrated into a chromosome of the plant, and is not found in a non-transgenic plant; and wherein said soybean plant exhibits an improvement in nematode disease resistance that results from suppression of an endogenous soybean DND1 gene.

8. A composition comprising a dsRNA molecule of 25 to about 95 nucleotides in length, wherein the dsRNA comprises a segment of 25 contiguous nucleotides that are identical or complementary to nucleic acid residues 1149 to 1806 of SEQ ID NO: 21, wherein said polynucleotide is not operably linked to a promoter or to a viral vector; and a transfer agent that conditions a plant surface to permeation by the dsRNA into cells of the plant.

9. The composition of claim 8 , wherein said dsRNA molecule is selected from the group consisting of SEQ ID NO: 38, 39, 43, and 45.

10. The composition of claim 8 , wherein said composition further comprises a non-polynucleotide herbicidal molecule, a polynucleotide herbicidal molecule, a polynucleotide that suppresses an herbicide target gene, an insecticide, a fungicide, a nematocide, or a combination thereof.

11. The composition of claim 8 , wherein said transfer agent is an organosilicone preparation.

12. A soybean plant comprising an exogenous dsRNA molecule of 25 to about 95 nucleotides in length, wherein the dsRNA comprises a segment of 25 contiguous nucleotides that are identical or complementary to nucleic acid residues 1149 to 1806 of SEQ ID NO: 21, wherein said exogenous dsRNA is not operably linked to a promoter or to a viral vector, is not transcribed from DNA integrated into a chromosome of the plant, is not found in a non-transgenic soybean plant; and wherein said soybean plant exhibits an improvement in nematode disease resistance that results from suppression of a endogenous DND1 gene.

13. The soybean plant of claim 12 , wherein said soybean plant further comprises an organosilicone compound or a component thereof.

14. The soybean plant of claim 12 , wherein said dsRNA is selected from the group consisting of SEQ ID NO: 38, 39, 43, and 45.

15. The method of claim 1 , wherein said dsRNA of 25 to about 95 nucleotides in length comprises a sequence selected from the group consisting of SEQ ID NO: 38, 39, 43, and 45.

16. The composition of claim 8 , wherein said dsRNA molecule of 25 to about 95 nucleotides in length comprises a sequence selected from the group consisting of SEQ ID NO: 38, 39, 43, and 45.

17. The soybean plant of claim 12 , wherein said dsRNA molecule of 25 to about 95 nucleotides in length comprise a sequence selected from the group consisting of SEQ ID NO: 38, 39, 43, and 45.

Assignees

• Monsanto Technology Llc

Inventors

• Crawford Michael J
• Gasper Michelle L
• Li Xiangqian
• Shortt Barry J
• Williams Deryck Jeremy

Classifications

• C12N15/8282

  for fungal resistance · CPC title

• C12N15/8218 Primary

  Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title

• C12N15/8285

  for nematode resistance · CPC title

• Y02A40/164

  Cross-Sectional Technologies · mapped topic

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title