Compositions and methods for solid phase extraction of lipids

Patent metadata

Field	Value
Publication number	US-10434492-B2
Application number	US-78516010-A
Country	US
Kind code	B2
Filing date	May 21, 2010
Priority date	Jan 26, 2007
Publication date	Oct 8, 2019
Grant date	Oct 8, 2019

Abstract

A composition, method and device for the preparation of biological samples for subsequent instrumental analyses, such as GC, GC-MS, LC and LC-MS analysis, using a solid phase extraction (SPE) process is described. Through SPE process alone or an integrated combination of protein precipitation, filtration, and SPE using a hydrophobic zirconia-coated chromatographic media, interfering compounds, such as proteins, glycerides and phosphate-containing compounds, are eliminated from the biological, food, environmental and biotechnology samples, affording an enhanced analyte response during the instrumental analysis.

First claim

What is claimed is:

1. A solid phase extraction (SPE) media for selective removal of lipids from a sample comprising:
a. a porous silica substrate with any one or more of a particle size ranging from about 10 nm to about 1000 μm, a pore size ranging from about 30 Å to about 1000 Å, and a surface area ranging from about 5 m 2 /g to about 1000 m 2 /g;
b. a hydrophobic alkyl linker having an attached end and a free end opposite the attached end, the attached end covalently bonded to the substrate, wherein the alkyl ranges from C 3 to C 18 ; and,
c. zirconia bonded to the porous silica substrate through a bridging bond.

2. The SPE media of claim 1 , wherein the sample comprises a biological sample, a food matrix, an environmental sample, a sample from a biotechnology process, or any combination thereof.

3. The SPE media of claim 1 , wherein the sample further comprises an acidic analyte, a non-acidic analyte, or a mixture of an acidic analyte and a non-acidic analyte.

4. A solid phase extraction (SPE) media for selective removal of lipids from a sample comprising:
a. a porous silica substrate with any one or more of a particle size ranging from about 10 nm to about 1000 μm, a pore size ranging from about 30 Å to about 1000 Å, and a surface area ranging from about 5 m 2 /g to about 1000 m 2 /g;
b. a hydrophobic alkyl linker comprising an attached end covalently bonded to the substrate and a free end opposite the attached end, wherein the alkyl ranges from C 3 to C 18 ; and,
c. zirconia covalently bonded through a bridging bond coupled to the free end of the hydrophobic alkyl linker.

5. The SPE media of claim 4 , wherein the sample comprises a biological sample, a food matrix, an environmental sample, a sample from a biotechnology process, or any combination thereof.

6. The SPE media of claim 4 , wherein the sample further comprises an acidic analyte, a non-acidic analyte, or a mixture of an acidic analyte and a non-acidic analyte.

7. A solid phase extraction (SPE) media for selective removal of lipids from a sample, the SPE comprising a mixture of:
a. a first particle comprising zirconia bonded through a bridging bond to a porous silica substrate having any one or more of a particle size ranging from about 10 nm to about 1000 μm, a pore size ranging from about 30 Å to about 1000 Å, and a surface area ranging from about 5 m 2 /g to about 1000 m 2 /g,
b. a second particle comprising a hydrophobic alkyl linker ranging from C 3 to C 18 having an attached end and a free end opposite the attached end, the attached end covalently bonded to a second porous silica substrate, the second porous silica substrate having any one or more of a particle size ranging from about 10 nm to about 1000 μm, a pore size ranging from about 30 Å to about 1000 Å, and a surface area ranging from about 5 m 2 /g to about 1000 m 2 /g.

8. The SPE media of claim 7 , wherein the sample comprises a biological sample, a food matrix, an environmental sample, a sample from a biotechnology process, or any combination thereof.

9. The SPE media of claim 7 , wherein the sample further comprises an acidic analyte, a non-acidic analyte, or a mixture of an acidic analyte and a non-acidic analyte.

Assignees

• Lu Xiaoning
• Shimelis Olga I
• Ye Maochun M
• Sigma Aldrich Co Llc

Inventors

• Lu Xiaoning
• Shimelis Olga I
• Ye Maochun M

Classifications

• B01J20/103

  comprising silica · CPC title

• B01J20/06 Primary

  comprising oxides or hydroxides of metals not provided for in group B01J20/04 · CPC title

• G01N30/466

  with separation columns in parallel · CPC title

• G01N30/6091

  Cartridges · CPC title

• B01J20/0211

  Compounds of Ti, Zr, Hf · CPC title